Jaoko WG, Lund M, Michael E & Simonsen PE (2001) A simple and quick method for enhanced detection of specific IgE in serum from lymphatic filariasis patients. Acta Tropica 80, 51-57.

Citation:
W. PROFJAOKOGODFREY. "Jaoko WG, Lund M, Michael E & Simonsen PE (2001) A simple and quick method for enhanced detection of specific IgE in serum from lymphatic filariasis patients. Acta Tropica 80, 51-57.". In: Acta Tropica 80, 51-57. AIDS 24(6):891-7; 2001.

Abstract:

MRC Human Immunology Unit, University of Oxford, Oxford, UK. The IFN-y enzyme-linked immunospot (ELI-Spot) assay is often used to map HIV-specific CD8 T-cell responses. We compared overlapping 15-mer pools with optimized CD8 epitopes to screen ELISpot responses in HIV-infected individuals. The 15-mer pools detected responses to previously undefined epitopes, but often missed low-level responses to predefined epitopes, particularly when the epitope was central in the 15-mer, rather than at the N-terminus or C-terminus. These factors should be considered in the monitoring of HIV vaccine trials.

Notes:

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