Beattie T, Kaul R, Rostron T, Dong T, Easterbrook P, Jaoko WG, Kimani J, Plummer F, McMichael A & Rowland-Jones S (2004) Screening for HIV-specific T-cell responses using overlapping 15-mer peptide pools or optimized epitopes. AIDS, 18:1-4.

Citation:
W. PROFJAOKOGODFREY. "Beattie T, Kaul R, Rostron T, Dong T, Easterbrook P, Jaoko WG, Kimani J, Plummer F, McMichael A & Rowland-Jones S (2004) Screening for HIV-specific T-cell responses using overlapping 15-mer peptide pools or optimized epitopes. AIDS, 18:1-4.". In: AIDS, 18:1-4. AIDS 24(6):891-7; 2004.

Abstract:

MRC Human Immunology Unit, University of Oxford, Oxford, UK. The IFN-y enzyme-linked immunospot (ELI-Spot) assay is often used to map HIV-specific CD8 T-cell responses. We compared overlapping 15-mer pools with optimized CD8 epitopes to screen ELISpot responses in HIV-infected individuals. The 15-mer pools detected responses to previously undefined epitopes, but often missed low-level responses to predefined epitopes, particularly when the epitope was central in the 15-mer, rather than at the N-terminus or C-terminus. These factors should be considered in the monitoring of HIV vaccine trials.

Notes:

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