T. Gotohda, K. Ogasawara, P. P. Wambua and K. Onoe. Analysis of functional sites on a peptide antigen, in I.A. or I.E. - restricted T cell responses. International Immunology Vol. 3, No.6, 503, 1991.

Citation:
P.M. MRWAMBUAPETER. "T. Gotohda, K. Ogasawara, P. P. Wambua and K. Onoe. Analysis of functional sites on a peptide antigen, in I.A. or I.E. - restricted T cell responses. International Immunology Vol. 3, No.6, 503, 1991.". In: J Immunol. 1991 Jan 1;146(1):26-34. IAHS Press Wallingford, UK.; 1991.

Abstract:

It has been shown that two different sites (an agretope and an epitope) on a peptide antigen function independently in T cell responses to the antigen. By virtue of these sites, antigens, MHC molecules, and TCRs constitute trimolecule complexes which eventually result in T cell activation. In our previous reports, we have defined that residues 46 and 54 on synthetic peptide composed of residues 43-58 of pigeon cytochrome c (p43-58, AEGFSYTDANKNKGIT) and its analogs function as an agretope and residue 50 as an epitope in both I-Ab and I-Ak-carrying mice. In the present study, to extend our method to the other MHC class II molecules (I-E), we used two peptide antigens, 46D50V54R and 50V54R, which had been prepared by substitution of amino acids at positions, 46, 50 and 54 or 50 and 54 of p43-58 D, V, R or V, R, respectively, and compared the immunogenicity with those of other peptide analogs. The 46D50V54R was shown to be non-immunogenic in I-Ab-carrying mice and the 50V54R was non-immunogenic in I-Ak-carrying mice. In contrast, the 46D50V54R or 50V54R could induce I-E-restricted proliferative responses of T lymphocytes in I-Eb/k- or I-Ek/k-carrying mice, respectively. Furthermore, residues 46 and 54 were shown to function as agretopes and residue 50 as an epitope in the I-E-restricted responses as they did in the I-A-restricted responses, even though some differences were seen between peptide-I-E interaction and peptide-I-A interaction. These agretopes and epitope functioned independently.

Notes:

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