Clonal population expansion in an outbreak of Plasmodium falciparum on the northwest coast of Ecuador

Citation:
Okoth S. "Clonal population expansion in an outbreak of Plasmodium falciparum on the northwest coast of Ecuador." Malaria journal. 2015;14(1):497.

Abstract:

Abstract
Background: Determining the source of malaria outbreaks in Ecuador and identifying remaining transmission
foci will help in malaria elimination efforts. In this study, the genetic signatures of Plasmodium falciparum isolates,
obtained from an outbreak that occurred in northwest Ecuador from 2012 to 2013, were characterized.
Methods: Molecular investigation of the outbreak was performed using neutral microsatellites, drug resistance markers
and pfhrp2 and pfhrp3 genotyping.
Results: A majority of parasite isolates (31/32) from this outbreak were of a single clonal type that matched a clonal
lineage previously described on the northern coast of Peru and a historical isolate from Ecuador. All but one isolate
carried a chloroquine-resistant pfcrt genotype and sulfadoxine- and pyrimethamine-sensitive pfdhps and pfdhfr genotypes.
Pfmdr1 mutations were identified in codons 184 and 1042. In addition, most samples (97 %) showed presence
of pfhrp2 gene.
Conclusions: This study indicates that parasites from a single clonal lineage largely contributed to this outbreak and
this lineage was found to be genetically related to a lineage previously reported in the Peruvian coast and historical
Ecuadorian parasites.
Keywords: Plasmodium falciparum, Malaria, Ecuador, Outbreak, Microsatellite markers

Website

PreviewAttachmentSize
s12936-015-1019-2.pdf2.06 MB

UoN Websites Search