Publications

Found 8 results

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Journal Article
Wood CM, Bergman HL, Bianchini A, Laurent P, Maina J, Johannsson OE, Bianchini LF, Chevalier C, Kavembe GD, Papah MB, Ojoo RO. "Transepithelial potential in the Magadi tilapia, a fish living in extreme alkalinity.". 2012. Abstract

We investigated the transepithelial potential (TEP) and its responses to changes in the external medium in Alcolapia grahami, a small cichlid fish living in Lake Magadi, Kenya. Magadi water is extremely alkaline (pH = 9.92) and otherwise unusual: titratable alkalinity (290 mequiv L(-1), i.e. HCO(3) (-) and CO(3) (2-)) rather than Cl(-) (112 mmol L(-1)) represents the major anion matching Na(+) = 356 mmol L(-1), with very low concentrations of Ca(2+) and Mg(2+) (<1 mmol L(-1)). Immediately after fish capture, TEP was +4 mV (inside positive), but stabilized at +7 mV at 10-30 h post-capture when experiments were performed in Magadi water. Transfer to 250% Magadi water increased the TEP to +9.5 mV, and transfer to fresh water and deionized water decreased the TEP to -13 and -28 mV, respectively, effects which were not due to changes in pH or osmolality. The very negative TEP in deionized water was attenuated in a linear fashion by log elevations in [Ca(2+)]. Extreme cold (1 vs. 28°C) reduced the positive TEP in Magadi water by 60%, suggesting blockade of an electrogenic component, but did not alter the negative TEP in dilute solution. When fish were transferred to 350 mmol L(-1) solutions of NaHCO(3), NaCl, NaNO(3), or choline Cl, only the 350 mmol L(-1) NaHCO(3) solution sustained the TEP unchanged at +7 mV; in all others, the TEP fell. Furthermore, after transfer to 50, 10, and 2% dilutions of 350 mmol L(-1) NaHCO(3), the TEPs remained identical to those in comparable dilutions of Magadi water, whereas this did not occur with comparable dilutions of 350 mmol L(-1) NaCl-i.e. the fish behaves electrically as if living in an NaHCO(3) solution equimolar to Magadi water. We conclude that the TEP is largely a Na(+) diffusion potential attenuated by some permeability to anions. In Magadi water, the net electrochemical forces driving Na(+) inwards (+9.9 mV) and Cl(-) outwards (+3.4 mV) are small relative to the strong gradient driving HCO(3) (-) inwards (-82.7 mV). Estimated permeability ratios are P (Cl)/P (Na) = 0.51-0.68 and [Formula: see text] = 0.10-0.33. The low permeability to HCO(3) (-) is unusual, and reflects a unique adaptation to life in extreme alkalinity. Cl(-) is distributed close to Nernst equilibrium in Magadi water, so there is no need for lower P (Cl). The higher P (Na) likely facilitates Na(+) efflux through the paracellular pathway. The positive electrogenic component is probably due to active HCO(3) (-) excretion.

Wood CM, Bergman HL, Bianchini A, Laurent P, Maina J, Johannsson OE, Bianchini LF, Chevalier C, Kavembe GD, Papah MB, Ojoo RO. "Transepithelial potential in the Magadi tilapia, a fish living.". 2005. Abstract

We investigated the transepithelial potential (TEP) and its responses to changes in the external medium in Alcolapia grahami, a small cichlid fish living in Lake Magadi, Kenya. Magadi water is extremely alkaline (pH = 9.92) and otherwise unusual: titratable alkalinity (290 mequiv L-1, i.e. HCO3 - and CO3 2-) rather than Cl-(112 mmol L-1) represents the major anion matching Na? = 356 mmol L-1, with very low concentrations of Ca2? and Mg2? (\1 mmol L-1). Immediately after fish capture, TEP was ?4 mV (inside positive), but stabilized at ?7 mV at 10–30 h post-capture when experiments were performed in Magadi water. Transfer to 250% Magadi water increased the TEP to ?9.5 mV, and transfer to fresh water and deionized water decreased the TEP to-13 and-28 mV, respectively, effects which were not due to changes in pH or osmolality. The very negative TEP in deionized water was attenuated in a linear fashion by log elevations in [Ca2?]. Extreme cold (1 vs. 28 C) reduced the positive TEP in Magadi water by 60%, suggesting blockade of an electrogenic component, but did not alter the negative TEP in dilute solution. When fish were transferred to 350 mmol L-1 solutions of NaHCO3, NaCl, NaNO3, or choline Cl, only the 350 mmol L-1 NaHCO3 solution sustained the TEP unchanged at ?7 mV; in all others, the TEP fell. Furthermore, after transfer to 50, 10, and 2% dilutions of 350 mmol L-1 NaHCO3, the TEPs remained identical to those in comparable dilutions of Magadi water, whereas this did not occur with comparable dilutions of 350 mmol L-1 NaCl— i.e. the fish behaves electrically as if living in an NaHCO3 solution equimolar to Magadi water. We conclude that the TEP is largely a Na? diffusion potential attenuated by some permeability to anions. In Magadi water, the net electrochemical forces driving Na? inwards (?9.9 mV) and Cl- outwards (?3.4 mV) are small relative to the strong gradient

Conference Paper
MARANDE PROFKISIASETH, OMONDI DROJOORODI, NDEGWA DRMAKANYAANDREW,. DRONYANGODANIELW. "Spermiogenesis and sperm ultrastructure of Lake Magadi tilapia Alcolapia grahami (Teleostei, Perciformes, Cichlidae).". In: Joint Faculty of Veterinary Medicine 8th Biennial Scientific Conference and 46th Kenya Veterinary Association Annual Scientific Conference. Journal of School of Continuous and Distance Education ; 2012. Abstract
Sudden death in the young after low energy anterior chest wall impact is an under-recognised phenomenon in this country. Review of the literature yields several American references to commotio cordis, mainly in the context of sporting events. Two cases are reported of sudden death in young men as a result of blunt impact anterior chest wall trauma. It is suggested that these cases draw attention to a lethal condition of which many practitioners are unaware. J Accid Emerg Med 2000 Nov 17 (6): 421-422. PMID: 11104247 [PubMed - indexed for MEDLINE]PMCID: PMC1725482  
OMONDI DROJOORODI. "Ojoo, R.O. (2006) Comparison and characterisation of specific fertilisation proteins in human (Homo sapiens) and baboon (Papio anubis) spermatozoa. Ph.D thesis , University of Nairobi.". In: TWAS-BiovisionAlexandria NXT Conference, Alexandria Egypt. Journal of School of Continuous and Distance Education ; 2006. Abstract
Sudden death in the young after low energy anterior chest wall impact is an under-recognised phenomenon in this country. Review of the literature yields several American references to commotio cordis, mainly in the context of sporting events. Two cases are reported of sudden death in young men as a result of blunt impact anterior chest wall trauma. It is suggested that these cases draw attention to a lethal condition of which many practitioners are unaware. J Accid Emerg Med 2000 Nov 17 (6): 421-422. PMID: 11104247 [PubMed - indexed for MEDLINE]PMCID: PMC1725482  
OMONDI DROJOORODI. "Ojoo, R.O., Otiang.". In: TWAS-BiovisionAlexandria NXT Conference, Alexandria Egypt. Journal of School of Continuous and Distance Education ; 2005. Abstract
Sudden death in the young after low energy anterior chest wall impact is an under-recognised phenomenon in this country. Review of the literature yields several American references to commotio cordis, mainly in the context of sporting events. Two cases are reported of sudden death in young men as a result of blunt impact anterior chest wall trauma. It is suggested that these cases draw attention to a lethal condition of which many practitioners are unaware. J Accid Emerg Med 2000 Nov 17 (6): 421-422. PMID: 11104247 [PubMed - indexed for MEDLINE]PMCID: PMC1725482  
OMONDI DROJOORODI. "Al-Eisa, N., Ojoo, R. O., & Moore, H. D. M. (2001) Characterisation of Human Sperm Antigens Exposed After the Acrosome Reaction That May Participate In Gamete Fusion. In: Andrology in the 21st Century; Proceedings of the VIIth International Congress of An.". In: TWAS-BiovisionAlexandria NXT Conference, Alexandria Egypt. Journal of School of Continuous and Distance Education ; 2001. Abstract
Sudden death in the young after low energy anterior chest wall impact is an under-recognised phenomenon in this country. Review of the literature yields several American references to commotio cordis, mainly in the context of sporting events. Two cases are reported of sudden death in young men as a result of blunt impact anterior chest wall trauma. It is suggested that these cases draw attention to a lethal condition of which many practitioners are unaware. J Accid Emerg Med 2000 Nov 17 (6): 421-422. PMID: 11104247 [PubMed - indexed for MEDLINE]PMCID: PMC1725482  
OMONDI DROJOORODI. "Ojoo, R.O. (1995) A morphological and endocrinological study of the male reproductive system of the thick-tailed bush baby (Galago garnetti). M.Sc. Thesis, University of Nairobi.". In: TWAS-BiovisionAlexandria NXT Conference, Alexandria Egypt. Journal of School of Continuous and Distance Education ; 1995. Abstract
Sudden death in the young after low energy anterior chest wall impact is an under-recognised phenomenon in this country. Review of the literature yields several American references to commotio cordis, mainly in the context of sporting events. Two cases are reported of sudden death in young men as a result of blunt impact anterior chest wall trauma. It is suggested that these cases draw attention to a lethal condition of which many practitioners are unaware. J Accid Emerg Med 2000 Nov 17 (6): 421-422. PMID: 11104247 [PubMed - indexed for MEDLINE]PMCID: PMC1725482  
OMONDI DROJOORODI. "Ojoo, R.O., Otiang.". In: TWAS-BiovisionAlexandria NXT Conference, Alexandria Egypt. Journal of School of Continuous and Distance Education ; 1994. Abstract
Sudden death in the young after low energy anterior chest wall impact is an under-recognised phenomenon in this country. Review of the literature yields several American references to commotio cordis, mainly in the context of sporting events. Two cases are reported of sudden death in young men as a result of blunt impact anterior chest wall trauma. It is suggested that these cases draw attention to a lethal condition of which many practitioners are unaware. J Accid Emerg Med 2000 Nov 17 (6): 421-422. PMID: 11104247 [PubMed - indexed for MEDLINE]PMCID: PMC1725482  

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