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24 weeks 5 days ago
RLE (RiP) normalisation with spike-in peaks in DiffBind
Hi,
I have two conceptual/technical questions about spike-in normalisation of ChIP-seq data.
We did spike-in for PolII ChIP-seq from a mouse cell line with ActiveMotif kit, which uses anti-H2Av antibody with drosophila cells.
So the mouse and fly...