Optical Immunosensor and ELISA for the Analysis of Pyrethroids and DDT in Environmental Samples

Citation:
Kremmer E, Krämer PM, Weber CM, Räuber C, Martens D, Forster S, Stanker LH, Rauch P, Shiundu PM, Mulaa FJ. Optical Immunosensor and ELISA for the Analysis of Pyrethroids and DDT in Environmental Samples.; 2007.

Abstract:

An optical immunosensor (AQUA-OPTOSENSOR) and ELISA (enzyme-linked immunosorbent assay) for the analysis of pyrethroids and DDT in river water and/or sediment, are described. The optical immunosensor consists of a bench-top optical read-out-device and disposable single-use sensor chips. ELISA was carried out in the coating antigen format. As examples, phenothrin (pyrethroid) and p,p'-DDT were chosen. Herein we describe the overall strategy, the set-up and principle of the immunosensor platform, and show representative results for immunosensor and ELISA analysis. The immunosensor employs fluorophore (Oyster®-645)-labeled monoclonal antibodies (mouse mAb Py-1 and rat mAb DDT 7C12), and makes use of the evanescent field, thus operating without washing steps. ELISA in the coating antigen format uses a second antibody labeled with peroxidase. Both, phenothrin and p,p'-DDT can be analyzed with these immunochemical techniques in the low ppb levels. Advantages and drawbacks of both immunochemical platforms are discussed.

Optical Immunosensor and ELISA for the Analysis of Pyrethroids and DDT in Environmental Samples

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