Biodegradation and detoxification of malachite green dye using novel enzymes from bacillus cereus strain KM201428: kinetic and metabolite analysis

Citation:
Wanyonyi WC, Onyari JM, Shiundu PM, Mulaa FJ. "Biodegradation and detoxification of malachite green dye using novel enzymes from bacillus cereus strain KM201428: kinetic and metabolite analysis." Energy Procedia. 2017;119:38-51.

Abstract:

Description
Enzyme based degradation of organic pollutants is a promising detoxifying approach due to the promiscuous nature of the enzyme, efficiency, cost effective and ecofriendly. In the present study, we have carried out detailed decoloration and degradation studies on a model triphenyl methane group of dyes (Malachite Green dye (MG)) using a newly isolated enzyme from Bacillus cereus KM201428 under the static condition. Biodegradation of dyes was monitored by UV-VIS spectrophotometer and the resultant metabolites analyzed by Liquid Chromatography–Hybrid Quadrupole Time of Flight Mass Spectrometry (LC–QToF-MS) and Gas Chromatography/Mass Spectrometry (GC - MS). Metabolite analysis results revealed that enzymatic degradation of MG dye resulted in complete mineralization and benzene ring-removal; the latter known for organic dye toxicity. Kinetic study results revealed that first-order kinetic …

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