Bio

Prof. Kinyanjui

I am a Professor of Biochemistry/Molecular Biology. I am also currently the Chairman, Department of Biochemistry. My research interest includes the use of Molecular Biology tools to address human and veterinary medical diseases such as trypanosomiasis, theilerosis and typhoid. I also have an interest in the use of DNA typing in forensic Science, and I am currently coordinating development of postgraduate Diploma course in Forensic Science in the department.

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Publications


2012

2011

2008

Kinyanjui, P, Bururia JM, Waiyaki PG, Kariuki SM, Karimi PN.  2008.  Plasmid Borne Resistance in Klebsiella Isolates from Kenyatta National Hospital, Nairobi, Kenya. Abstractplasmid_borne_resistance_in_klebsiella_isolates_from_kenyatta_national_hospital.pdf

Eighty six Klebsiella isolates from Kenyatta National Hospital and the Centre for Microbiology, Kenya Medical Research Institute, Nairobi were screened for resistance to commonly prescribed antimicrobial agents and for their plasmid content. Plasmids were transferred into Esherichia coli K-12 and resulting transconjugants screened for resistance to the antimicrobial agents used on Klebsiella donors and for their plasmid content. Plasmids from the Klebsiella isolates were also transformed into Eschericia coli and transformants analyzed for resistance and plasmid content. Endonuclease restriction mapping was done to characterize the plasmids from Klebsiella isolates and their Eschericia coli transformants. Resistance was found to be plasmid borne and transmissible.

WANJIRU, MRSKARIUKILUCY, KINYUA DRNGUUEDWARD, W. DRKINYANJUIPETER, BULIMO DRDIMBUSONWALLACE.  2008.  L.W. Kariuki, E.K. Nguu, R.M. Njogu, P. W. Kinyanjui, J.O.Midiwo, W.D. Bulimo, J.K.Kiaira. MAESANIN: A BENZOQUINONE FROM MAESA LANCEOLATA THAT COMPLETELY INHIBITS RESPIRATION IN BLOODSTREAM TRYPANOSOMA BRUCEI BRUCEI. 5th International Congress of the African Association of Physiological Sciences (AAPS). : Heinrich Boll Foundation.

2007

Kamau, JM, Mwai A, Kinyanjui PW, Iraqi FA.  2007.  Trypanotolerance effect as a result of genomic imprinting in F murine population. Abstract

African tsetse-fly transmitted trypanosomosis affects a wide range of wild and domesticated animal species. Trypanotolerance, the ability of some breeds to withstand the infection has been recognized and provides a sustainable option in animal production. While a genetic contribution, several behavioural traits are not in doubt, an attempt to find the responsible genes has proven to be complicated. One advance towards generating trypanotolerant animals has been the demonstration of an effective genetic im printing phenomenon in crossbred mice, similar to that observed following challenge. We report a novel reciprocal crossing strategy that exploits epistasis and heterosis in inbred mouse strains to identify imprinting effect controlling trypanosomosis using an F2 (129/ J x CS7BL/6) resource populations. The results indicate that genetic control for trypanotolerance is complicated and the identification of imprinting effect may provide new insights of introgressing trypanotolerance in livestock

2006

2005

2004

1991

W., DRKINYANJUIPETER.  1991.  Kinyanjui, P.W. and Pearlman, R.E. (1991). Thymidine kinase from Tetrehymena thermophilia. Purification of the enzyme and immunological analysis; Eur J. Biochem. 195, 55. Eur J. Biochem.. : Heinrich Boll Foundation. Abstractabstrac.pdf

Thymidine kinase is an enzyme involved in DNA precursor metabolism and DNA replication. The synthesis of this enzyme is highly regulated during the cell cycle and the activity of the enzyme is also regulated by feedback inhibition. Genes encoding thymidine kinase have been extremely useful as selectable markers for introducing DNA into a number of cells. In order to study cell cycle regulation of thymidine kinase, the gene which encodes this enzyme, as well as aspects of DNA replication in the ciliated protozoan Tetrahymena thermophila, we have purified thymidine kinase from Tetrahymena. Two forms of thymidine kinase with native molecular masses of 59 kDa and 80 kDa have been identified and purified 6800- and 4600-fold, respectively. The 59-kDa enzyme, a homodimer of 30-kDa subunits, has been purified to near homogeneity and polyclonal antibodies have been raised against the 30-kDa subunit. Serological studies indicate that the two enzymes are antigenically distinct. The antibody against the Tetrahymena protein cross-reacts with a polypeptide in Chinese hamster ovary (CHO) cell extracts of 26 kDa which corresponds to the reported size of Chinese hamster thymidine kinase protein.

1985

Bell, TM; Tukei, PM; AGR; MFM; GGW; MJM; KMHDT; AP; J; J, et al.  1985.  Investigation of the effectiveness of measles vaccination in children in Kenya.. Abstract

Laboratory studies were performed on 128 children clinically diagnosed as measles when seen at the Infectious Diseases Hospital, Kenyatta National Hospital (IDH), Nairobi (86 cases) and the Rural Health Training Centre, Maragua, Central Province (42 cases) between 9 July and 31 August 1984. A concurrent measles infection was confirmed in 95% of the children seen at IDH and in 85% of those seen at Maragua, with similar proportions of confirmations in children who had, and who had not, received measles vaccine. No differences in the number of sero-conversions nor in the absolute levels of acute or convalescent HI antibody titres could be detected between vaccinated and unvaccinated children. Analysis of the cases seen at Maragua indicates that about two thirds of the children who had received vaccine were protected. A pilot study of vaccinating children at 8 months and again at 12-13 months is suggested in an attempt to eradicate measles

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