Konrad L.1, Owmandho C.A.2 , von Hobe A.-K. 1, Sui C. 1, Kloeppels K1.,Hersemeyer K.1 Tinneberg H.-R 1 In proceedings of 14th World Congress of Gynecological Endocriology, ISGE. March 4-7, 2010, Firenza, Italy. 1 University of Giessen, Department of Gynec

Citation:
A PROFOMWANDHOCHARLESO. "Konrad L.1, Owmandho C.A.2 , von Hobe A.-K. 1, Sui C. 1, Kloeppels K1.,Hersemeyer K.1 Tinneberg H.-R 1 In proceedings of 14th World Congress of Gynecological Endocriology, ISGE. March 4-7, 2010, Firenza, Italy. 1 University of Giessen, Department of Gynec.". In: In proceedings of 14th World Congress of Gynecological Endocriology, ISGE. March 4-7, 2010, Firenza, Italy.; 2010.

Abstract:

Introduction: To establish  a model for investigationg endometrics in vitro, we analyzed several immortalized endometrial and endometriotic cell lines for typical biological functions. Especially transforming growth factor (TGF)-betas were investigated, because TGF-betas are increased during menstrual and endometriosis and seem to modulate effects of progesterone on endometrial cells primarily on secretion of matrix metalloproteinases (MMPs).Methods: We used two stroma and one epithelial cell line from human endometrium and one stromal and three epithelial cell lines from endometrial patients. Protein secretion was quantitated with ELISAs and apoptosis determined with FACS analysis.Results: All cell lines secrete TGF-beta1, but interestingly the endometriotic cell lines secret considerably higher levels compared to normal endometrial cells. Of note,not the endometriotic cells secrete TGF-beta2. However, all cell lines only produce very slow levels of TGF-beta3. All cell lines could be stimulated by TGF-bata because they express high-affinity receptors TBR1 and TBR2. Treatment with TGF-betas reduced cell proliferation by innducing apoptosis, whereas TGF-beta1 more efficiently increased secretion of MMP2 compared to TGF0beta2. Progesterone reduced cell proliferation dose-dependent but induced secretion of TGF-beta1 and TGF-beta2. Interestingly TGF-beta3 was predominantly found in endothelial cells of patients with endometriosis.Conclusion: The results obtained with the endometrial and endometriotic celss lines are highly consistent with published data. For example TGF-beta1 is the predominant TGF-beta isoform in explant cultures as well as in endometriosis, followed by TGF-beta2 and very low levels of TGF-beta3. Our results convincingly demonstrate that cell lines are a suitable model for studying endometriosis.

Notes:

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