B.K. AMUGUNE, G.N. THOITIDAND, I.0. KIBWAGE. Liquid chromatographic analysis of phenobarbitone, ethosuximide, phenytoin and carbamazepine on a polystyrene-divinyl benzene column

Citation:
"B.K. AMUGUNE, G.N. THOITIDAND, I.0. KIBWAGE. Liquid chromatographic analysis of phenobarbitone, ethosuximide, phenytoin and carbamazepine on a polystyrene-divinyl benzene column.". 2006.

Abstract:

A liquid chromatographic method for the simultaneous assay of four anticonvulsant
drugs, phenobarbitone, ethosuximide, phenytoin and carbamazepine on a
polystyrene-divinyl benzene column is described. The method was developed by the
systematic study of different types of co-polymer materials, type and concentration
of organic modifiers, buffer pH and concentration and column temperature. A
PLRP-S 100A 8 11mcolumn maintained at 60°C and a mobile phase consisting of
acetonitrile-tert-butanol-phosphate buffer (pH 7.6, 0.2 M)-water (25:5:10:60, v/v)
were used. The flow rate was 1 ml/min with ultraviolet detection at 220 nm. The
method has been validated and used for the analysis of raw materials, finished
products and dissolution studies of the drugs.

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