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Mwenesi BM. "L’enseignement du français dans le contexte multilingue Est-Africain et kenyan .". In: L’enseignement du français dans le contexte multilingue Est-Africain. Kenyatta University; 2011.ku_paper.pdf
Mier PD, van den Hurk JJ. "Lysosomal hydrolases of the epidermis. I. Glycosidases." Br. J. Dermatol.. 1975;93(1):1-10. Abstract

Seven distinct glycosidases (EC 3.2) have been characterized in guinea-pig epidermis. Their properties indicate them to be of lysosomal origin. The 'profile' of the epidermal glycosidases is significantly different from that reported for whole skin, the activities of beta-galactosidase and beta-acetylglucosaminidase being very high and those of the remaining enzymes relatively low in epidermis.

Mier PD, van den Hurk JJ. "Lysosomal hydrolases of the epidermis. 2. Ester hydrolases." Br. J. Dermatol.. 1975;93(4):391-8. Abstract

Five distinct ester hydrolases (EC 3-1) have been characterized in guinea-pig epidermis. These are carboxylic esterase, acid phosphatase, pyrophosphatase, and arylsulphatase A and B. Their properties are consistent with those of lysosomal enzymes.

Campbell KA, Lipinski MJ, Doran AC, Skaflen MD, Fuster V, McNamara CA. "Lymphocytes and the adventitial immune response in atherosclerosis." Circulation research. 2012;110:889-900. Abstract

Although much of the research on atherosclerosis has focused on the intimal accumulation of lipids and inflammatory cells, there is an increasing amount of interest in the role of the adventitia in coordinating the immune response in atherosclerosis. In this review of the contributions of the adventitia and adventitial lymphocytes to the development of atherosclerosis, we discuss recent research on the formation and structural nature of adventitial immune aggregates, potential mechanisms of crosstalk between the intima, media, and adventitia, specific contributions of B lymphocytes and T lymphocytes, and the role of the vasa vasorum and surrounding perivascular adipose tissue. Furthermore, we highlight techniques for the imaging of lymphocytes in the vasculature.

JOAB PROFBWAYOJOB. "Lymphocyte subsets in human immunodeficiency virus type 1-infected and uninfected children in Nairobi. Embree J, Bwayo J, Nagelkerke N, Njenga S, Nyange P, Ndinya-Achola J, Pamba H, Plummer F.Pediatr Infect Dis J. 2001 Apr;20(4):397-403.". In: Pediatr Infect Dis J. 2001 Apr;20(4):397-403. Asian Economic and Social Society; 2001. Abstract
Background. The host immune response against mucosally-acquired pathogens may be influenced by the mucosal immune milieu during acquisition. Since Neisseria gonorrhoeae can impair dendritic cell and T cell immune function, we hypothesized that co-infection during HIV acquisition would impair subsequent systemic T-cell responses.   Methods. Monthly screening for sexually transmitted infections (STIs) was performed in high risk, HIV seronegative Kenyan female sex workers as part of an HIV prevention trial. Early HIV-specific CD8+ T cell responses and subsequent HIV viral load set point were assayed in participants acquiring HIV, and were correlated with the presence of prior genital infections during HIV acquisition.   Results. Thirty-five participants acquired HIV during follow up, and 16/35 (46%) had a classical STI at the time of acquisition. N. gonorrhoeae co-infection was present during HIV acquisition in 6/35 (17%), and was associated with an increased breadth and magnitude of systemic HIV-specific CD8+ T-cell responses, using both interferon- (IFNg) and MIP-1 beta (MIP1b) as an output. No other genital infections were associated with differences in HIV-specific CD8+ T cell response, and neither N. gonorrhoeae nor other genital infections were associated with differences in HIV plasma viral load at set point.   Conclusion. Unexpectedly, genital N. gonorrhoeae infection during heterosexual HIV acquisition was associated with substantially enhanced HIV-specific CD8+ T-cell responses, although not with differences in HIV viral load set point. This may have implications for the development of mucosal HIV vaccines and adjuvants.
Levin AG, Jones M, Kirkham DM, Shah T, Peters TJ, Hill ID, Wasunna A, Brubaker G. "Lymphocyte enzyme activities in East African blacks: decrease in 5'nucleotidase and possible relation to immunosuppression." Trans. R. Soc. Trop. Med. Hyg.. 1983;77(6):840-4. Abstract

Microanalysis of subcellular organelle marker enzymes was applied to cryopreserved lymphocytes (obtained and processed in the field) from East African blacks with moderate to severe malnutrition and subject to locally endemic parasitic and infectious diseases. An initial study demonstrated that activities of these enzymes, with the partial exception of catalase, were stable to cryopreservation. Cryopreserved and thawed lymphocyte specimens (1 to 3 X 10(6) viable cells) from 26 Africans and 20 Caucasian controls were studied. There was a highly significant decrease in 5'nucleotidase activity in these African subjects. Activity of another plasma membrane enzyme, gamma-glutamyl transferase, and of marker enzymes for other intracellular organelles, was not significantly different between the two groups, indicating that the nucleotidase alteration is highly specific. 5'Nucleotidase activity in a group of 17 East African blacks of high socio-economic status lay between the values obtained in the other two groups and was not significantly different from either. Further studies on 5'nucleotidase showed no evidence that the enzyme is functionally different in Africans. The differences in activity of this enzyme in Africans may reflect the known immuno-suppressive effects of infectious disease and malnutrition or may have a genetic basis which may in turn be associated with the pathogenesis of secondary immunodeficiency.

and 1. Elias R. M., G. Wandolo REJNSJMG. "Lymphatic Pumping in response to changes in transmural pressure is modulated by erythrolysate/haemoglobin." Circulation Research 1990. 1990;(67):1097-1106. Abstract

1) Elias R. M., G. Wandolo, N. S. Ranadive, J. Eisenhoffer and M. G. Johnston: Lymphatic Pumping in response to changes in transmural pressure is modulated by erythrolysate/haemoglobin. Circulation Research 1990; 67: 1097 – 1106. - 1990
Department of Pathology, University of Toronto, Ontario, Canada.
Red blood cells and lysate products (erythrolysate) are observed consistently in lymph draining acute and chronic inflammatory reactions and from tissues subjected to trauma or surgical procedures. Using hemoglobin as a marker for erythrolysate, we have measured hemoglobin in lymph up to the 10(-6) M range in a number of pathophysiological states. Data demonstrate that erythrolysate alters the pumping characteristics of lymphatic vessels. To test the effects of erythrolysate on lymphatic pumping, bovine lymphatics were suspended in an organ bath preparation with the vessels cannulated at both inflow and outflow ends. By raising the heights of the Krebs reservoir and the outflow catheters appropriately, a transmural pressure that stimulated pumping activity could be applied to the vessels. With a fixed transmural pressure of 6 cm H2O applied to the ducts, sheep erythrolysate depressed pumping activity between 40% and 100%, with dilutions containing between 10(-8) and 10(-5) M hemoglobin. Although the active principle in the red blood cells has not been characterized, evidence from precipitation purification experiments suggests that hemoglobin is an important component. Once suppressed, pumping could be restored in many but not all vessels (often to control levels) by elevating the distending pressure above 6 cm H2O. The relation between transmural pressure and fluid pumping is expressed as a bell-shaped curve, with pumping increasing up to a peak pressure (usually 8 cm H2O) and declining at pressures above this level. By comparing pressure/flow curves, we were able to ascertain that hemoglobin shifted the lymphatic function curve to the right and, on average, reduced the maximum pumping capability of the vessels. We speculate that the presence of erythrolysate/hemoglobin in lymph may modulate the ability of lymphatic vessels to drain liquid and protein from the tissue spaces.
Circulation Research 1990; 67: 1097 – 1106.

Naidu SI, McCalla MR. "Lymphatic malformations of the head and neck in adults: a case report and review of the literature." Annals of Otology, Rhinology & Laryngology. 2004;113:218-222. AbstractWebsite
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de Casso MC, Lewis NJ, Rapado F. "Lymphangioma presenting as a neck mass in the adult." International journal of clinical practice. 2001;55:337-338. AbstractWebsite
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OLE PROFMALOIYGEOFFREYM. "LYKKEBOE, G., JOHANSEN, K. and MALOIY, G.M.O. (1975). Functional properties of haemoglobin in the teleost, Tilapia grahami.Journal of Comparative Physiology 104, 1-11.". In: Proceedings of the 7th Pan-African Ornithological Congress, p. 17. EAMJ; 1975. Abstract
Serum acid phosphatase was measured in patients with enlarged benign and malignant prostate before and after rectal examination. Amongst the patients with benign glands, rectal examination did not produce any significant false elevation of the enzyme. Rectal examination, however, caused a rise in the enzyme level in a few untreated cancer patients and in cancer patients who has become refractory to hormonal therapy. This rise would help rather than mislead in the diagnosis of malignant prostate and also in the identifying treated patients who had become refractory to treatment. Thus, when serum acid phosphatase is properly determined, elevated levels should always arouse suspicion of malignant prostate or other lesions associated with high enzyme level even is such determination was preceded by rectal examination. There appears to be no merit in the teaching that the determination of serum acid phosphatase should be delayed after rectal examination.
Otieno SP, Ngang'a E. Lwanda. Githinji K, ed. Talent Empire Kenya; 2008.
OLONDE PROFAMAYOERASTUS, OTIENO PROFOGUTUELLY. "Lwai-Lume L, Ogutu EO, Amayo EO, Kariuki S.Drug susceptibility pattern of Helicobacter pylori in patients with dyspepsia at the Kenyatta National Hospital, Nairobi.East Afr Med J. 2005 Dec;82(12):603-8.". In: East Afr Med J. 2005 Dec;82(12):603-8. Journal of British Ceramic Transactions, 99 [5], 206-211.; 2005. Abstract
OBJECTIVE: To determine drug susceptibility pattern of Helicobacter pylori to metronidazole, clarithromycin, amoxicillin and tetracycline in patients presenting with dyspepsia at the Kenyatta National Hospital. DESIGN: Cross-sectional descriptive study. SETTING: Kenyatta National Hospital, Nairobi. SUBJECTS: Two hundred and sixty-seven patients aged 15 to 85 years, presenting with dyspepsia and referred for upper gastro-intestinal endoscopy were recruited into the study. RESULTS: Between October 2003 and April 2004, 138 male and 129 female patients aged 15-85 years, with a mean age of 45.4 years were studied. Gastritis was the most common endoscopic finding, occurring in 55%, followed by normal-looking mucosa in 27% and peptic ulcer disease in 16% of the patients. The rapid urease test was positive in 184 patients (69%). The culture yield was 62% of these CLO (Campylobacter like organisms) positive biopsies. The MIC90 (minimum inhibitory concentration) was 256 mg/l for metronidazole, 1.5 mg/l for clarithromycin, 1.5 mg/l for tetracycline and 0.75 mg/l for amoxicillin. The MIC values for amoxicillin were significantly higher in the female patients (p = 0.02) but showed no significant variation for age. The MIC values for metronidazole, tetracycline and clarithromycin showed no significant difference for age or gender. MIC values for tetracycline were significantly higher for patients with duodenitis and duodenal ulcer p = 0.009 and 0.02, respectively. CONCLUSION: All isolated H. pylori organisms were resistant to metronidazole. The susceptibility of the H. pylori isolates was 93.6% for clarithromycin, 95.4% for amoxicillin and 98.1% for tetracycline. The MIC90 for amoxicillin and clarithromycin were found to be close to the upper limit of the susceptibility range. There was a rising MIC90 for tetracycline and metronidazole compared to that found in a previous study in 1991.
M. PROFLUTIFELIX. "Luti, F.M. (2002). Fundamentals of Engineering Thermodynamics, University of Nairobi Press, Nairobi, ISBN 9966846530, 385 pp.". In: East Afr Med J. 1999 May;76(5):281-3. UN-HABITAT; 2002. Abstract
Galactosaemia is a disorder of galactose metabolism in which raised levels of galactose and galactose-l-phosphate damage various organs. It is a very rare disease (incidence 1 in 60,000) and the diagnosis is often missed, leading to poor prognosis. A case of clinical galactosaemia that was diagnosed at the age of 11 months is reported. It is important to be aware of this condition as early treatment may prevent some of the complications.
M. PROFLUTIFELIX. "Luti, F.M. (1987). Experimental modelling of axial temperature profiles of buoyant diffusion flames. African Journal of Science and Technology, Series A, vol. 6, no. 1, 76-80.". In: East Afr Med J. 1999 May;76(5):281-3. UN-HABITAT; 1987. Abstract
Mean temperatures were measured along the axis of propane gas flames using a porous ceramic burner. Data for fire heat release rates ranging form 13.3kW to 30.6kW were taken and analyzed. It was found that the lower zone of a fire in a clam environment could be conveniently divided into three regions. For the same heat release rates, the fires were subjected to a cross flow of 0.72ms-1 and it was found that along the axis, the three zones could be identified. The excess temperature in the intermittent zone decreased with height to the power of 0.60 for the calm environment and 1.11 with crossflow. In the plume zone, excess temperature decreased with 1.87 power for the clam case and 2.05 with crossflow case, all data when plotted on log plots fell on a single curve.
M. PROFLUTIFELIX. "Luti, F.M. (1987). Experimental modelling of axial temperature profiles of buoyant diffusion flames. African Journal of Science and Technology, Series A, vol. 6, no. 1, 76-80.". In: East Afr Med J. 1999 May;76(5):281-3. UN-HABITAT; 1987. Abstract
Mean temperatures were measured along the axis of propane gas flames using a porous ceramic burner. Data for fire heat release rates ranging form 13.3kW to 30.6kW were taken and analyzed. It was found that the lower zone of a fire in a clam environment could be conveniently divided into three regions. For the same heat release rates, the fires were subjected to a cross flow of 0.72ms-1 and it was found that along the axis, the three zones could be identified. The excess temperature in the intermittent zone decreased with height to the power of 0.60 for the calm environment and 1.11 with crossflow. In the plume zone, excess temperature decreased with 1.87 power for the clam case and 2.05 with crossflow case, all data when plotted on log plots fell on a single curve.
M. PROFLUTIFELIX. "Luti, F.M. (1980). Transient flow development due to a strong heat source in the atmosphere; Part I: Uniform temperature source. Combustion Science and Technology, 23, pp 163-75.". In: East Afr Med J. 1999 May;76(5):281-3. UN-HABITAT; 1980. Abstract

This paper describes a finite difference experiment to simulate the transient development of the convection column above a strip of uniform high temperature source in a stratified uniform cross flow atmosphere. The k-e model of turbulence is used and an upstream weighted scheme for this vorticity-stream function formulation is employed. Some computation results for a 195m wide source are presented. The immediate goal of the undertaking was to devise a scheme which could yield realistic flow 'boundary conditions' in the immediate vicinity of a fire to enable one to model the detailed structure of the fire region. The predictions are quite consistent with observations and this provides the link between the heat source and environmental parameters. This scheme, together with a refined near zone fire model, can be used to describe the interplay between the heat source (fire) component and the environmental components of a mass fire system.

S PROFKIGONDUCHRISTINE. "Lutalo-Bosa A.J., Sekadde C.B., Opiyo W. and Kiwanuka J.B., Presentation of paper to the Annual Scientific Conference of East Africa Medical Research Council, February, 1977 Liver function tests among normal Ugandans. A paper was reported in the Annual Re.". In: Annual Scientific Conference of East Africa Medical Research Council. uon press; 1977. Abstract

PIP: Masturbatory semen specimen from 49 fertile Black African males in Kenya whose wives were pregnant was obtained after at least 3 days of abstinence for the analysis of parameters which included volume motility, vitality, sperm concentration, pH, fructose and acid phosphatase levels. About 1/2 the spermatozoa was actively progressive in motility, while 40% was nonmotile. Vitality in the 1st hour revealed that 81.4% of the sperm was alive. About 90% of the semen specimens had more than 40% idea forms of spermatozoa. Spermatozoal abnormalities were a frequent feature. There was no correlation between age and the testicular volume, but seminal fluid volume and sperm density tended to decrease with age. Serum levels of Follicle Stimulating Hormone, Luteinizing Hormone, Prolactin and Testosterone were determined in the subjects' sera, enabling the establishment of reference values for these parameters in African Kenyan males.

JOAB PROFBWAYOJOB, O. PROFANZALAAGGREY. "Luscher MA, Choy G, Njagi E, Bwayo JJ, Anzala AO, Ndinya-Achola JO, Ball TB, Wade JA, Plummer FA, Barber BH, MacDonald KS.Naturally occurring IgG anti-HLA alloantibody does not correlate with HIV type 1 resistance in Nairobi prostitutes.AIDS Res Hum Retro.". In: AIDS Res Hum Retroviruses. 1998 Jan 20;14(2):109-15. Asian Economic and Social Society; 1998. Abstract
In an effort to identify an immunological basis for natural resistance to HIV-1 infection, we have examined serum antibody responses to HLA class I antigens in female prostitutes of the Nairobi Sex Workers Study. Anti-HLA antibodies are known to block HIV infectivity in vitro and can be protective against SIV challenge in macaques immunized with purified class I HLA. Thus, it was postulated that broadly cross-reactive alloantibodies recognizing common HLA alleles in the client population might contribute to the prevention of heterosexual transmission of HIV. In fact, 12% of the women were found to have serum IgG antibodies against class I alloantigens. However, this alloantibody did not correlate with the HIV status of the women and was found in a similar proportion of HIV-positive and HIV-resistant women. The observed levels of alloantibody did not increase with HIV infection in susceptible individuals, suggesting that potential antigenic mimicry between HIV and host HLA class I antigens does not significantly increase levels of anti-class I antibodies. The lack of correlation between serum anti-allo-class I HLA antibodies and the risk of sexual transmission indicates that this humoral immune response is unlikely to be the natural mechanism behind the HIV-resistance phenotype of persistently HIV-seronegative women. This result, however, does not preclude the further investigation of alloimmunization as an artificial HIV immunization strategy.
JOAB PROFBWAYOJOB, O. PROFANZALAAGGREY. "Luscher MA, Choy G, Njagi E, Bwayo JJ, Anzala AO, Ndinya-Achola JO, Ball TB, Wade JA, Plummer FA, Barber BH, MacDonald KS.Naturally occurring IgG anti-HLA alloantibody does not correlate with HIV type 1 resistance in Nairobi prostitutes.AIDS Res Hum Retro.". In: AIDS Res Hum Retroviruses. 1998 Jan 20;14(2):109-15. John Benjamins Publishing Company; 1998. Abstract
In an effort to identify an immunological basis for natural resistance to HIV-1 infection, we have examined serum antibody responses to HLA class I antigens in female prostitutes of the Nairobi Sex Workers Study. Anti-HLA antibodies are known to block HIV infectivity in vitro and can be protective against SIV challenge in macaques immunized with purified class I HLA. Thus, it was postulated that broadly cross-reactive alloantibodies recognizing common HLA alleles in the client population might contribute to the prevention of heterosexual transmission of HIV. In fact, 12% of the women were found to have serum IgG antibodies against class I alloantigens. However, this alloantibody did not correlate with the HIV status of the women and was found in a similar proportion of HIV-positive and HIV-resistant women. The observed levels of alloantibody did not increase with HIV infection in susceptible individuals, suggesting that potential antigenic mimicry between HIV and host HLA class I antigens does not significantly increase levels of anti-class I antibodies. The lack of correlation between serum anti-allo-class I HLA antibodies and the risk of sexual transmission indicates that this humoral immune response is unlikely to be the natural mechanism behind the HIV-resistance phenotype of persistently HIV-seronegative women. This result, however, does not preclude the further investigation of alloimmunization as an artificial HIV immunization strategy.
B. PROFESTAMBALEBENSON. "Luoba AI, Wenzel Geissler P, Estambale B, Ouma JH, Alusala D, Ayah R, Mwaniki D, Magnussen P, Friis H. Earth-eating and reinfection with intestinal helminths among pregnant and lactating women in western Kenya. Trop Med Int Health. 2005 Mar;10(3):220-7.". In: Trop Med Int Health. 2005 Mar;10(3):220-7. Taylor & Francis; 2005. Abstract
We conducted a longitudinal study among 827 pregnant women in Nyanza Province, western Kenya, to determine the effect of earth-eating on geohelminth reinfection after treatment. The women were recruited at a gestational age of 14-24 weeks (median: 17) and followed up to 6 months postpartum. The median age was 23 (range: 14-47) years, the median parity 2 (range: 0-11). After deworming with mebendazole (500 mg, single dose) of those found infected at 32 weeks gestation, 700 women were uninfected with Ascaris lumbricoides, 670 with Trichuris trichiura and 479 with hookworm. At delivery, 11.2%, 4.6% and 3.8% of these women were reinfected with hookworm, T. trichiura and A. lumbricoides respectively. The reinfection rate for hookworm was 14.8%, for T. trichiura 6.65, and for A. lumbricoides 5.2% at 3 months postpartum, and 16.0, 5.9 and 9.4% at 6 months postpartum. There was a significant difference in hookworm intensity at delivery between geophagous and non-geophagous women (P=0.03). Women who ate termite mound earth were more often and more intensely infected with hookworm at delivery than those eating other types of earth (P=0.07 and P=0.02 respectively). There were significant differences in the prevalence of A. lumbricoides between geophagous and non-geophagous women at 3 (P=0.001) and at 6 months postpartum (P=0.001). Women who ate termite mound earth had a higher prevalence of A. lumbricoides, compared with those eating other kinds of earth, at delivery (P=0.02), 3 months postpartum (P=0.001) and at 6 months postpartum (P=0.001). The intensity of infections with T. trichiura at 6 months postpartum was significantly different between geophagous and non-geophagous women (P=0.005). Our study shows that geophagy is associated with A. lumbricoides reinfection among pregnant and lactating women and that intensities built up more rapidly among geophagous women. Geophagy might be associated with reinfection with hookworm and T. trichiura, although these results were less unequivocal. These findings call for increased emphasis, in antenatal care, on the potential risks of earth-eating, and for deworming of women after delivery.
B. PROFESTAMBALEBENSON. "Luoba AI, Geissler PW, Estambale B, Ouma JH, Magnussen P, Alusala D, Ayah R, Mwaniki D, Friis H. Geophagy among pregnant and lactating women in Bondo District, western Kenya. Trans R Soc Trop Med Hyg. 2004 Dec;98(12):734-41.". In: Trans R Soc Trop Med Hyg. 2004 Dec;98(12):734-41. Taylor & Francis; 2004. Abstract
Geophagy was studied among 827 pregnant women in western Kenya, during and after pregnancy. The women were recruited at a gestational age of 14-24 weeks and followed-up to 6 months post-partum. The median age (range) of the women was 23 years and median parity 2. At recruitment, 378 were eating earth, of which most (65%) reported earth-eating before pregnancy. The preferred type of earth eaten was soft stone, known locally as odowa (54.2%) and earth from termite mounds (42.8%). The prevalence remained high during pregnancy, and then declined to 34.5% and 29.6% at 3 and 6 months post-partum respectively (P < 0.001). The mean daily earth intake was 44.5 g during pregnancy, which declined to 25.5 g during lactation (P < 0.001). A random sample of 204 stools was collected from the women and analysed for silica content as a tracer for earth-eating. The mean silica content was 2.1% of the dry weight of stool. Geophagous women had a higher mean silica content than the non-geophagous ones (3.1% vs. 1.4%, P < 0.001). Faecal silica and reported geophagy were strongly correlated (P < 0.001).
PROF. KOKWARO JOHNO. "Luo-English Botanical Dictionary, Nairobi,.". In: First Edition. 4to. Hard cover. Dust jacket. Study of medicinal plants and their uses in East Africa. Pp. (xii),384, photos, line-drawings. Some light browning. University of Nairobi Press; 1972. Abstract
Total number of pages: 515, including 35 pages of colour illustrations.
Kokwaro JO, Johns T. Luo-English Biological Dictionary. Nairobi: East African Educational Publishers Ltd; 2013.
OKOTH PROFOKOMBODUNCAN. "Luo Meets Latin: Linguistic Evidence for Contacts Between Speakers of Pre-Dholuo and Speakers of Lation.' In Proceeding of the Third Nilo-Saharan Linguistic Colloquim. Hamburg Buske Verlag.". In: A book review in Journal of African Languages and Linguistics Vol. 15 - 1, 80-85. CIGR Electocic Journal; 1991. Abstract
isolated from preterm neonates during the outbreak of gastroenteritis in hospital in Nairobi, Kenya, were resistance to trimethoprin-sulfathoxaxole, Chloramphenicol, oxytetracycline and ampicilin, but only a few strains were resistant to cefazolin, cefamandole, cefataximine, amikacin and nalidixic acid. Fourteen different antimicrobial resistance patterns were observed in the 229 strains of E.coli analyzed. Eighty-two percent of the EPEC strains belonged to two resistance patterns. There was no consistent relationship between palsmid profile group and antimicrobial resistance pattern, although one resistance pattern was more frequently observed in EAF-positive strins belonging to the dominant plasmid profile group. Nine percent of the EPEC strins were resistant to gentamicin compared to 37% in the non-EPEC group. No correlation was observed between administration of gentamicin and percentage of resistant strains isolated. None of the nine neonates receiving gentamicin died during the outbreak. Gentamicin resistance was observed in E.coli strains from six out of these nine neonates. Five out of fourteen neonates who received other antimicrobials, or no antibiotic at all, died. Key words: Enteropathogenic Escherichia Coli; antimicrobial resistance;
O. PROFNDINYA-ACHOLAJ. "Luo M, Embree J, Ramdahin S, Ndinya-Achola J, Njenga S, Bwayo JB, Pan S, Mao X, Cheang M, Stuart T, Brunham RC, Plummer FA.HLA-A and HLA-B in Kenya, Africa: allele frequencies and identification of HLA-B*1567 and HLA-B*4426.Tissue Antigens. 2002 May;59(5).". In: Tissue Antigens. 2002 May;59(5):370-80. IBIMA Publishing; 2002. Abstract
HLA-A and HLA-B alleles of a population from Kenya, Africa were examined by sequencing exon 2 and exon 3 DNA and typing using a Taxonomy-based Sequence-analysis (TBSA) method. Extensive diversities were observed at both HLA-A and HLA-B loci in this population. Forty-one HLA-A alleles were identified from 159 unrelated individuals. The most frequently observed alleles were A*6802 (11.64%), A*02011/09 (9.75%), A*7401/02 (9.43%), A*3001 (7.86%), A*3002 (7.23%) and A*3601 (6.6%). Forty-nine HLA-B alleles were identified in 161 unrelated individuals, including two novel alleles, B*1567 and B*4426. The most frequently observed HLA-B alleles were B*5301 (9.01%), B*5801 (8.38%), B*4201 (7.76%), B*1503 (7.14%), B*1801 (6.21%), and B*5802 (5.90%). The most frequently observed HLA-A-B haplotypes were A*3601-B*5301 (3.55%) and A*3001-B*4201 (3.19%), followed by A*7401/02-B*5801 (2.84%), A*7401/02-B*5802 (2.84%) and A*02011/09-B*1503 (2.13%). Linkage disequilibrium and chi2 analysis showed the association of these HLA-A-B haplotypes at the antigen level to be significant. The frequencies of HLA-A and HLA-B alleles from the Kenyan population were compared with that of a population from Cameroon. The difference in allele and haplotype frequency distributions partly reflected the different ethnic composition of these two African populations.
Nyarwath O. "The Luo Care for Widows (Lako) and Contemporary Challenges." Thought and Practice: A Journal of the Philosophical Association of Kenya. 2012;4(1):91-110.
PROF. KOKWARO JOHNO. "Luo Biological Dictionary; 264pp 1998 East African Educ. Publ.". In: Journal Of Ethnobiology Volume 25, Issue 1 (March 2005) Pp. 129. University of Nairobi Press; 1998. Abstract
Total number of pages: 515, including 35 pages of colour illustrations.
PROF. KOKWARO JOHNO. "Luo Biological Dictionary (Jointly authored with Prof. Timothy Johns of McGill University, Canada).". In: A well researched ethnobiological lexicon describing all the plants and animals known to and used by the Luo community in East Africa. University of Nairobi Press; 1998. Abstract
Total number of pages: 515, including 35 pages of colour illustrations.
Butt FMA, Guthua SW, Waweru W, Kiarie G. "Lung Bronchogenic Carcinoma in the mandible: A rare metastasis." The Annals of African Surgery. 2016;13 (1):32-35.
Ogeng’o JA, Ongeti KW, Kilonzi J, Maseghe P, Murunga A, Machira J, Mburu E. "Luminal Dimensions of left Anterior Descending Coronary Artery in a Black Kenyan population." Anat Physiol. 2013;3(123).
HAMU PROFHABWEJOHN. Lulu ya Maisha. Jomo Kenyatta Foundation; 2013.
N PROFLULEGODFREY. "Lule GN.Spontaneous bacterial peritonitis. East Afr Med J. 2004 Mar;81(3):113. Review. No abstract available.". In: East Afr Med J. 2005 Apr;82(4):166-9. test; 2004. Abstract

OBJECTIVE: To determine the prevalence of HCV infection and HCV/HIV co-infection among voluntary blood donors at the National Blood Transfusion Centre and clients at the Kenyatta National Hospital HIV-Voluntary Counseling and Testing (VCT) Centre. DESIGN: A prospective cross-sectional descriptive study. SETTING: Kenyatta National Hospital, a tertiary referral and teaching hospital and the National Blood Transfusion Services Centre, Nairobi. SUBJECTS: Volunteer blood donors and VCT attendants. RESULTS: The prevalence of HCV/HIV co-infection among 6154 blood donors in the NBTSC was very low, at 0.02. The HIV prevalence among the 353 KNH HIV-VCT clients was 9.3%, none of the clients tested positive for HCV. The incidence of risk factors in the persons with HCV and/or HIV infection(s) was low. CONCLUSION: The prevalence of HCV infection among pre-screened volunteer blood donors was low. However the current practice of screening all donated blood for HCV remains indispensable to prevent its transmission to blood recipients.

N PROFLULEGODFREY. "Lule GN. Helicobacter pylori: an infectious agent in peptic ulcer disease? East Afr Med J. 1991 May;68(5):321-3. No abstract available.". In: East Afr Med J. 1991 May;68(5):321-3. test; 1991. Abstract

The range of clinical presentations of HIV-related disease in Africa has not been adequately described, despite the fact that many hospitals have to rely heavily on clinical diagnosis. Six hundred adult medical patients seen in the Casualty Department of the main Government hospital in Nairobi were enrolled in a study of the presentation and outcome of HIV-related disease: 506 of these patients were admitted, of whom 19 per cent (95) were HIV seropositive. The remaining 94 were dealt with as outpatients: 11 percent (10) of these were seropositive. A history of prior treatment for sexually transmitted disease and, if male, being uncircumcised, were associated with being seropositive. Three presentations were strongly associated with HIV infection: acute fever with no focus except the gastrointestinal tract (enteric fever-like illness), acute cough with fever (community-acquired pneumonia) and chronic diarrhoea with wasting. The WHO clinical case definition (CCD) for AIDS missed a substantial amount of HIV-related morbidity (sensitivity 39 per cent) and misidentified many seronegative patients (positive predictive value 59 per cent). In comparison with the Centers for Disease Control surveillance definition for AIDS, the CCD was specific (91 per cent) and sensitive (79 per cent) but only had a positive predictive values of 30 per cent: the CCD may therefore be a poor surveillance tool for AIDS. Seropositive patients were much more likely to die than were seronegative patients (39 per cent vs. 15 per cent mortality). Enteric fever-like illness was the presentation which most commonly proved fatal. A wider spectrum of disease is associated with underlying HIV immunosuppression than has previously been described in Africa.

N PROFLULEGODFREY. "Lule GN. Helicobacter pylori eradication in peptic dyspepsia. East Afr Med J. 2005 Dec;82(12):601-2. No abstract available.". In: East Afr Med J. 2005 Dec;82(12):601-2. test; 2005. Abstract

OBJECTIVE: To evaluate the utility of Total Lymphocyte Count (TLC) as a surrogate marker for CD4 + T cell count in antiretroviral (ARV) treatment initiation in a Kenyan population of HIV seropositive patients at Kenyatta National Hospital. DESIGN: Cross-sectional descriptive study. SETTING: Kenyatta National Hospital, HIV treatment and follow-up outpatient facility; Comprehensive Care Centre, Nairobi, Kenya. SUBJECTS: Two hundred and twenty five HIV Elisa positive, ARV naive patients visiting the Comprehensive Care Centre between January 2006 to March 2006. RESULTS: A significant linear correlation was found between TLC and CD4 cell count for the whole group with a Spearman rank correlation of 0.761 (p < 0.01); and was also independently observed in the four WHO clinical stages. The classification utility of TLC 1200 cells/mm3 cut-off was suboptimal; sensitivity 37% specificity of 99% and the NPV of 56%. The receiver operator characteristics (ROC) curve generated an optimal TLC cut-off of 1900 cells/mm3 cut-off to be of greatest utility with a sensitivity of 81.1%, specificity of 90.3%, PPV of 90.8% and NPV of 80.2%. This implies that a TLC cut-off of 1900 cells/mm3 correctly classify eight out of ten HIV positive patients as having a CD4 < 200 cells/mm3 and only misclassify two such patients. Serial CD4 testing can then be performed on the minority of patients who despite a TLC > or = 1900 cells/mm3 are, on basis of clinical data, suspect of more advanced disease warranting ARV therapy. This would reduce the number of patients tested for and focus the application of CD4 testing and thus reduce attendant cost in care provision in CD4 resource poor settings. CONCLUSION: Our data showed a good positive correlation between TLC and CD4 cell count, however the WHO recommended TLC cuto-ff of 1200/mm3 was found to be of low sensitivity in classifying patients as having a CD4 counts < 200 cells/mm3. This would result in underestimation of advanced stage of disease and to withholding ARVs treatment to persons who need treatment. We recommend a TLC cut-off of 1900 cells/mm3 for our population to classify patients as either above or below the CD4 count cut-off of 200 cells/mm3 as an indicator of when to start antiretroviral therapy.

N PROFLULEGODFREY. "Lule GN, Wankya BM. Duodenal ulcer disease at Kenyatta National Hospital. East Afr Med J. 1985 Nov;62(11):784-90. No abstract available.". In: East Afr Med J. 1985 Nov;62(11):784-90. test; 1985. Abstract

A case of cryptococcal hepatitis is described in a patient with Human Immunodeficiency Virus (HIV) infection. There has been no previous reports of this in the East African literature. A review of world literature showed that the entity of cryptococcal hepatitis is very rare.

N PROFLULEGODFREY. "Lule GN, Wankya BM, Shah MV, Greenfield C. Peptic ulcer disease at Kenyatta National Hospital: an endoscopic experience. East Afr Med J. 1987 Oct;64(10):638-42. No abstract available.". In: East Afr Med J. 1987 Oct;64(10):638-42. test; 1987. Abstract

A case of cryptococcal hepatitis is described in a patient with Human Immunodeficiency Virus (HIV) infection. There has been no previous reports of this in the East African literature. A review of world literature showed that the entity of cryptococcal hepatitis is very rare.

N PROFLULEGODFREY. "Lule GN, Sang F, Ogutu EO.Helicobacter pylori in peptic ulcer disease in Kenya.East Afr Med J. 1991 May;68(5):324-7.". In: East Afr Med J. 1991 May;68(5):324-7. test; 1991. Abstract

Sixty six patients with dyspeptic symptoms underwent upper gastrointestinal endoscopy and biopsies for Helicobacter pylori culture. The number of H. pylori isolated increased with age reaching a peak at 51 to 60 years of age. Antral gastritis closely followed by duodenitis accounted for the highest number of H. pylori isolated, (87.5% and 85.7% respectively). In patients with duodenal ulceration only, 57% had H. pylori isolated from their antral biopsies, a result that was just slightly higher than that where no endoscopic diagnosis was made (50%).

N PROFLULEGODFREY. "Lule GN, Otieno LS, Kinuthia DM. Hypertension over a 4-year-period at Kenyatta National Hospital (KNH). East Afr Med J. 1985 Jun;62(6):365-71. No abstract available.". In: East Afr Med J. 1985 Jun;62(6):365-71. test; 1985. Abstract

A case of cryptococcal hepatitis is described in a patient with Human Immunodeficiency Virus (HIV) infection. There has been no previous reports of this in the East African literature. A review of world literature showed that the entity of cryptococcal hepatitis is very rare.

OTIENO PROFOGUTUELLY. "Lule GN, Okoth F, Ogutu EO, Mwai SJ.HBV markers (HBsAg, HBSAb, HBCAb in 160 medical students at Kenyatta National Hospital. East Afr Med J. 1989 May;66(5):315-8.". In: East Afr Med J. 1989 Jan;66(1):10-4. Journal of British Ceramic Transactions, 99 [5], 206-211.; 1989. Abstract
In an exercise to study the immunogenicity and reactogenicity of the Engerix B vaccine, 160 medical students were screened for Hepatitis B Markers (HBsAg, HBsAb and HBcAb) with a view to vaccinating those that were negative. 18% were HBsAg + ve, 33% were HBsAb + ve and 38% were positive for the HBcAb. These figures were extremely high and obviated the need to vaccinate the students as they begin their clinical years.
N PROFLULEGODFREY. "Lule GN, Okoth F, Ogutu EO, Mwai SJ. HBV markers (HBsAg, HBSAb, HBCAb in 160 medical students at Kenyatta National Hospital. East Afr Med J. 1989 May;66(5):315-8.". In: East Afr Med J. 1989 May;66(5):315-8. test; 1989. Abstract

In an exercise to study the immunogenicity and reactogenicity of the Engerix B vaccine, 160 medical students were screened for Hepatitis B Markers (HBsAg, HBsAb and HBcAb) with a view to vaccinating those that were negative. 18% were HBsAg + ve, 33% were HBsAb + ve and 38% were positive for the HBcAb. These figures were extremely high and obviated the need to vaccinate the students as they begin their clinical years.

OTIENO PROFOGUTUELLY. "Lule GN, Ogutu EO, Okoth F, Were JB, Wambugu M.Sclerotherapy in the treatment of bleeding oesophageal varices: preliminary report. East Afr Med J. 1988 Jun;65(6):416-20. Review. No abstract available.". In: East Afr Med J. 1989 Dec;66(12):830-3. Journal of British Ceramic Transactions, 99 [5], 206-211.; 1988. Abstract
The mean fasting insulin level in 30 apparently normal Kenyan Africans was determined by Radio-Immuno Assay (RIA) technique based on coat A-count. The mean value was found as 16.33 microIu/ml with a standard deviation (s.d) of 10.51 microIu/ml. The mean at 95% confidence interval (CI) for the population studied was 16.33 +/- 1.92. The findings are similar to what have been reported among the caucasians. This paper also reports on a case of insulinoma in a young female patient at Kenyatta National Hospital (KNH).
N PROFLULEGODFREY. "Lule GN, Ogutu EO, Okoth F, Were JB, Wambugu M. Sclerotherapy in the treatment of bleeding oesophageal varices: preliminary report. East Afr Med J. 1988 Jun;65(6):416-20. Review. No abstract available.". In: East Afr Med J. 1988 Jun;65(6):416-20. test; 1988. Abstract

A case of cryptococcal hepatitis is described in a patient with Human Immunodeficiency Virus (HIV) infection. There has been no previous reports of this in the East African literature. A review of world literature showed that the entity of cryptococcal hepatitis is very rare.

N DRWAMBUGUMILCAH. "Lule GN, Ogutu EO, Okoth F, Were JB, Wambugu M. Sclerotherapy in the treatment of bleeding oesophageal varices: preliminary report. East Afr Med J. 1988 Jun;65(6):416-20. Review.". In: East Afr Med J. 1988 Jun;65(6):416-20. Review. University of Nairobi.; 1988. Abstract
A prospective study was carried out at Kenyatta National Hospital (KNH) between June 1987 and September 1988 to look at some aspects of obstructive jaundice in patients above 12 years of age. Screening for cases was done by use of abdominal ultrasonography. A total of 20 cases (11 females, 9 males) were diagnosed. Carcinoma of the head of pancreas accounted for 55% of cases of the obstruction, followed by gallstones (10%), hepatocellular carcinoma (10%) and gall bladder tumour (10%).
N PROFLULEGODFREY, OTIENO PROFOGUTUELLY. "Lule GN, Obiero ET, Ogutu EO.Factors that influence the short term outcome of upper gastrointestinal bleeding at Kenyatta National Hospital. East Afr Med J. 1994 Apr;71(4):240-5.". In: East Afr Med J. 1994 Jan;71(1):44-8. Journal of British Ceramic Transactions, 99 [5], 206-211.; 1994. Abstract
Between April 1990 to January 1991, a prospective study of 97 consecutive patients admitted to the medical wards of Kenyatta National Hospital with upper gastrointestinal haemorrhage was done. All the patients studied under went upper gastrointestinal endoscopy within 24 hours of presentation and a positive endoscopic diagnosis was possible in 90 (93%) of the patients studied. The leading causes of bleeding were oesophageal varices in 34 (35%) patients, duodenal ulceration in 17 (17.5%) patients, and superficial inflammatory lesions in 17 (17.5%) patients. 17 (17.5%) patients had multiple lesions with superficial inflammation and oesophageal varices as the commonest combination. In 86 (88%) patients, bleeding settled on simple conservative measures which included intravenous fluids, blood transfusion and antacids. Further haemorrhage was observed in 11 (11.3%) patients. Tachycardia (> 100/minute), hypotension (systolic BP < 100mm Hg) and low haemoglobin (< 8 g/dl) at admission were all correlated with a poor outcome. Variceal bleeding had the worst prognosis and the overall mortality rate was 5%.
N PROFLULEGODFREY, OTIENO PROFOGUTUELLY. "Lule GN, Obiero ET, Ogutu EO.Factors that influence the short term outcome of upper gastrointestinal bleeding at Kenyatta National Hospital. East Afr Med J. 1994 Apr;71(4):240-5.". In: East Afr Med J. 1994 Jan;71(1):44-8. test; 1994. Abstract

Between April 1990 to January 1991, a prospective study of 97 consecutive patients admitted to the medical wards of Kenyatta National Hospital with upper gastrointestinal haemorrhage was done. All the patients studied under went upper gastrointestinal endoscopy within 24 hours of presentation and a positive endoscopic diagnosis was possible in 90 (93%) of the patients studied. The leading causes of bleeding were oesophageal varices in 34 (35%) patients, duodenal ulceration in 17 (17.5%) patients, and superficial inflammatory lesions in 17 (17.5%) patients. 17 (17.5%) patients had multiple lesions with superficial inflammation and oesophageal varices as the commonest combination. In 86 (88%) patients, bleeding settled on simple conservative measures which included intravenous fluids, blood transfusion and antacids. Further haemorrhage was observed in 11 (11.3%) patients. Tachycardia (> 100/minute), hypotension (systolic BP < 100mm Hg) and low haemoglobin (< 8 g/dl) at admission were all correlated with a poor outcome. Variceal bleeding had the worst prognosis and the overall mortality rate was 5%.

N PROFLULEGODFREY, OLONDE PROFAMAYOERASTUS. "Lule GN, Amayo EO. Irritable bowel syndrome in Kenyans. East Afr Med J. 2002 Jul;79(7):360-3.". In: East Afr Med J. 2002 Jul;79(7):360-3. African Wildlife Foundation. Nairobi; 2002. Abstract

OBJECTIVE: To study the prevalence and clinical presentation of irritable bowel syndrome (IBS) in black Kenyans. DESIGN: A retrospective study. SETTING: Nairobi Hospital, an urban private institution. SUBJECTS: Case files of all patients presenting with abdominal pain. MAIN OUTCOME MEASURES: Prevalence of IBS using modified Manning's Symptomatic criteria in 223 consecutive adult patients presenting with abdominal pain and discomfort who had normal clinical findings and normal general and gastrointestinal investigations. RESULTS: Out of the 3472 patient files screened, there were 281 patients who fitted the diagnosis of irritable bowel syndrome giving an overall prevalence of 8%. The mean age was 40 years with range of 11 to 75 years with a peak prevalence in the 3rd decade. The male to female ratio of 1.4:1 being statistical significant at a p-value of 0.010. There was no statistical significant difference between the males and females in constipation and diarrhoea at p-value of 0.84 and 0.82 respectively. Fifteen percent of the patients were either on antidepressants or had been seen by a psychiatrist. Nine percent had undergone laparatomy for the abdominal pain. CONCLUSION: The Black African patient is equally as exposed to irritable bowel syndrome as is his counterpart in the western world and has similar morbidity patterns. The syndrome may account for over 10% of patients with abdominal pain presenting to gastroenterologists in Africa. Proper attention to symptomatology may lead to clinching the diagnosis without resorting to many unnecessary and expensive investigations.

N PROFLULEGODFREY, OLONDE PROFAMAYOERASTUS. "Lule GN, Amayo EO. Irritable bowel syndrome in Kenyans. East Afr Med J. 2002 Jul;79(7):360-3.". In: East Afr Med J. 2002 Jul;79(7):360-3. test; 2002. Abstract

OBJECTIVE: To study the prevalence and clinical presentation of irritable bowel syndrome (IBS) in black Kenyans. DESIGN: A retrospective study. SETTING: Nairobi Hospital, an urban private institution. SUBJECTS: Case files of all patients presenting with abdominal pain. MAIN OUTCOME MEASURES: Prevalence of IBS using modified Manning's Symptomatic criteria in 223 consecutive adult patients presenting with abdominal pain and discomfort who had normal clinical findings and normal general and gastrointestinal investigations. RESULTS: Out of the 3472 patient files screened, there were 281 patients who fitted the diagnosis of irritable bowel syndrome giving an overall prevalence of 8%. The mean age was 40 years with range of 11 to 75 years with a peak prevalence in the 3rd decade. The male to female ratio of 1.4:1 being statistical significant at a p-value of 0.010. There was no statistical significant difference between the males and females in constipation and diarrhoea at p-value of 0.84 and 0.82 respectively. Fifteen percent of the patients were either on antidepressants or had been seen by a psychiatrist. Nine percent had undergone laparatomy for the abdominal pain. CONCLUSION: The Black African patient is equally as exposed to irritable bowel syndrome as is his counterpart in the western world and has similar morbidity patterns. The syndrome may account for over 10% of patients with abdominal pain presenting to gastroenterologists in Africa. Proper attention to symptomatology may lead to clinching the diagnosis without resorting to many unnecessary and expensive investigations.

OTIENO PROFOGUTUELLY. "Lule G.N., Sang F., Ogutu E.O. Helicobacter Pylori in peptic ulcer disease. E. Afr. Med. J. 68: 324, 1991.". In: East Afr Med J. 1991 May;68(5):324-7. Journal of British Ceramic Transactions, 99 [5], 206-211.; 1991. Abstract
Department of Medicine, University of Nairobi. Sixty six patients with dyspeptic symptoms underwent upper gastrointestinal endoscopy and biopsies for Helicobacter pylori culture. The number of H. pylori isolated increased with age reaching a peak at 51 to 60 years of age. Antral gastritis closely followed by duodenitis accounted for the highest number of H. pylori isolated, (87.5% and 85.7% respectively). In patients with duodenal ulceration only, 57% had H. pylori isolated from their antral biopsies, a result that was just slightly higher than that where no endoscopic diagnosis was made (50%).
DR. LUKHOBA CATHERINEW. "Lukhoba, C., 2000. Medicinal plants: Their role and future. First Symposium on EastAfrica in Transition: Communities, Cultures and Change, 4th-7th July 2000, Nairobi.". In: First Symposium on EastAfrica in Transition: Communities, Cultures and Change, 4th-7th July 2000, Nairobi.; 2000.
DR. LUKHOBA CATHERINEW. "Lukhoba, C. & Paton, A.J., 2000. Two new species of Plectranthus L.". In: Kew Bulletin, 55 (4): 957-964.; 2000.
DR. LUKHOBA CATHERINEW. "Lukhoba, C and Siboe G., 2008. Ethnobotanical data in the search and identification of drug plants. East and Central African Journal of Pharmaceutical Sciences. 11: 43-48.". In: East and Central African Journal of Pharmaceutical Sciences.; 2008. Abstract
Traditional medicine has utilized plants to palliate, cure and/or prevent diseases in both humans and animals. The acquisition of knowledge has been through trial and error, and observation. Today, the enhanced search for botanical drugs throughout the world has increased the need for accurate means of identifying plants with possible pharmacological and biological activity. A number of methodologies have been used in selecting plants likely to possess pharmacological properties, but many have recorded low success rates. Data reported in this paper reveal that the accuracy of identification of these herbal drugs for pertinent ailments using ethnobotanical data is almost as accurate as techniques applied in modern medical practice. This paper discusses the value of ethno-botanical data in the preliminary search for potential drug plants Key words: Ocimum, Plectranthus, ethnobotany, medicinal plants.
DR. LUKHOBA CATHERINEW. "Lukhoba C.W. and Midiwo, J.O. 2001. Kenyan aromatic plants with a potential for economic utilization. First National Workshop On Medicinal, Aromatic and Other Under-utilised Plant Species in Kenya, 29th October- 3rd November, 2001.". In: First National Workshop On Medicinal, Aromatic and Other Under-utilised Plant Species in Kenya, 29th October- 3rd November, 2001.; 2001.
DR. LUKHOBA CATHERINEW. "Lukhoba C.W. 2001. Taxonomic revision of the genera Ocimum L. and Plectranthus L.". In: Ph. D. Thesis, University of Nairobi.; 2001. Abstract

Ocimum L. and Plectranthus L'Hér. are cosmopolitan genera in the tribe Nepeteoideae in the family Labiatae Juss. The accumulation of essential oils in their flower and foliage has contributed to their prominence and popularity in traditional and conventional medicine, in cosmetology and perfumery, in the food industry as preservatives and spices, and more recently in the manufacturing industry as ingredients for pesticides, plastics, paints, etc.

The taxonomic aspect of the group has lagged well behind the economic one. The latest world- wide account of the two genera was by Briquet (1895-7), more than 100 years ago. In both these taxa, the taxonomic delimitation has been inadequate, the major problem being the continuous nature of the variation of characters particularly the morphological ones, which results in difficulties in circumscription of species. To further complicate the situation, Ocimum species tend to hybridize readily and undergo polymorphism. Morphological characters have proved to be inadequate in delimitation of the genera. It is therefore imminent that other criteria be investigated to substitute or corroborate the morphological ones in the delimitation of these two genera.
The major aim of this project was therefore to find novel morphological characters or novel combinations of already known characters and the value of new criteria from phytochemical data and anatomy to augment the morphological data in the delimitation of Ocimum and Plectranthus. Field and herbarium collections were used in this study. Gas-liquid chromatography/mass spectrometry (GLC/MS) on the essential oils from field samples were conducted in the chemotaxonomic study while the surface anatomical characters of Plectranthus were analyzed using the scanning electron microscope (SEM). In the revision of Ocimum, new morphological characters have been identified that can differentiate between sections and even between species, for instance, in the dendrite-haired species of subsection Gratissima, O. cufodontii and O. jamesii have n-shaped while O. spicatum have bowl-shaped anthers. Morphological features separating O. basilicum and O. americanum were established. In Plectranthus, three new species namely, P. agnewii C. Lukhoba & A. Paton, P. xylopodus C. Lukhoba & A. Paton sp. nov. and P. kwalensis C. Lukhoba & J. Kokwaro as well as the varieties P. barbatus var. glabricalyx C. Lukhoba & J. Kokwaro and P. edulis var. longiflora C. Lukhoba & J. Kokwaro have been described. One name change, P. igniarius var. verdcourtii C. Lukhoba & J. Kokwaro formerly P. igniarius var. grandicalyx) has been made. The chemical analysis conducted on four Ocimum and eleven Plectranthus species revealed the presence of numerous essential oil constituents. Some compounds were species-specific, some genera-specific while others were common to both genera thus can be of taxonomic value. The species O. basilicum, O. kilimandscharicum and O. kenyense clustered together in the cluster analysis. These three species have traditionally been grouped together in the section Ocimum. O. gratissimum showed greater affinity to Plectranthus than to Ocimum. The Plectranthus species came out as one homogenous group with two subgroups. The anatomical analysis of Plectranthus showed the indumentum to have a large number of sessile glands (peltate, gland dots), some capitate glands and numerous multi-cellular eglandular hairs. The viscid species P. kamerunensis and P. agnewii had the highest number of capitate glands. The presence, structure and nature of the stomata and glands were of taxonomic importance. Seven species had amphistomatic stomata and another seven species had stomata on the stems. The stratification of the leaf and stem surfaces was also found to be taxonomically significant. Cluster analysis showed that members of subgenus Calceolanthus clustered together into one group. They were also distinct from subgenus Plectranthus. A key based on these anatomical characters was drawn up to identify species in Plectranthus. Ethnomedicinal data revealed that Ocimum and Plectranthus species are popularly used to cure or alleviate gastro-intestinal, febrile, respiratory and skin conditions. Four species namely, O. basilicum, O. kilimandscharicum, O. gratissimum and P. barbatus showed high potential for further pharmacological evaluation, and for incorporation into local health care systems. This data also revealed that the medicinal species in the two genera are greatly affected by the current degradation of the environment. Thus propagation of the potentially medicinal ones was recommended. It is recommended that future taxonomic revisions of the two genera should include chemotaxonomic and anatomical data as they have proved to be promising criteria. Members of the two genera that have substantial amounts of essential oils can be commercially utilized in industry. The promising medicinal Ocimum and Plectranthus species can be incorporated into the primary health care systems. It is hoped that the new criteria used in this study will be relevant for the on-going revision of the family Labiatae for the Flora of Tropical East Africa.

WANJIRU DRNGUGIROSE, Ngugi RW. "Luka Kandie Kiptui, Exchange rate pass-through to domestic prices in Kenya: 1972-2004, University of Nairobi, 2005." The Icfai University Journal of Architecture, Vol. II No.1, February 2010; 2004.
IRIBEMWANGI PI, Makanji N. "Lugha-Kati kama Mchakato wenye Manufaa: Kifani cha Matumizi ya Kiswahili na Wazungumzaji wa Kikisa kama Lugha ya Kwanza." Jarida la Kimataifa la Isimu ya Kibantu (JAKIIKI). Journal of Bantu Linguistics. 2019;(Special):114-128.
SWALEH AMIRI. "Lugha ya Kiswahili Pijini au Krioli ?" aasisi ya Uchunguzi wa Kiswahili, Dar es Salaam. 2002.
J DRCHWEYALUDEKI. "Ludeki Chweya, .". In: Proceedings of the Africa Regional Development Policy Forum, 10-11 June 1998, Nairobi, Kenya, 153-164. UNCRD Proceedings Series, No. 29 (Nagoya: United Nations Centre for Regional Development, 1998). ISCTRC; 2004. Abstract
Differentiation of bloodstream-form trypanosomes into procyclic (midgut) forms is an important first step in the establishment of an infection within the tsetse fly. This complex process is mediated by a wide variety of factors, including those associated with the vector itself, the trypanosomes and the bloodmeal. As part of an on-going project in our laboratory, we recently isolated and characterized a bloodmeal-induced molecule with both lectin and trypsin activities from midguts of the tsetse fly, Glossina longipennis [Osir, E.O., Abubakar, L., Imbuga, M.O., 1995. Purification and characterization of a midgut lectin-trypsin complex from the tsetse fly, Glossina longipennis. Parasitol. Res. 81, 276-281]. The protein (lectin-trypsin complex) was found to be capable of stimulating differentiation of bloodstream trypanosomes in vitro. Using polyclonal antibodies to the complex, we screened a G. fuscipes fuscipes cDNA midgut expression library and identified a putative proteolytic lectin gene. The cDNA encodes a putative mature polypeptide with 274 amino acids (designated Glossina proteolytic lectin, Gpl). The deduced amino acid sequence includes a hydrophobic signal peptide and a highly conserved N-terminal sequence motif. The typical features of serine protease trypsin family of proteins found in the sequence include the His/Asp/Ser active site triad with the conserved residues surrounding it, three pairs of cysteine residues for disulfide bridges and an aspartate residue at the specificity pocket. Expression of the gene in a bacterial expression system yielded a protein (M(r) approximately 32,500). The recombinant protein (Gpl) bound d(+) glucosamine and agglutinated bloodstream-form trypanosomes and rabbit red blood cells. In addition, the protein was found to be capable of inducing transformation of bloodstream-form trypanosomes into procyclic forms in vitro. Antibodies raised against the recombinant protein showed cross-reactivity with the alpha subunit of the lectin-trypsin complex. These results support our earlier hypothesis that this molecule is involved in the establishment of trypanosome infections in tsetse flies.
GICHOHI DRMBUTHIAPAUL. "Lucy Wanjiru Njagi, Paul Gichohi Mbuthia , Phillip Njeru Nyaga, Lilly Caroline Bebora, Uswege M. Minga. 2011. Viral nucleoprotein localization and lesions of Newcastle disease in tissues of indigenous ducks.Trop Anim Health Prod DOI 10.1007/s11250-011-995.". In: Livestock research for Rural development. Kenya Veterinary association; 2011. Abstract
bstract Localization of Newcastle disease viral nucleoprotein and pathological lesions was evaluated in tissues of 55 indigenous ducks (45 experimentally infected and 10 sentinel ones). In addition, ten Newcastle disease infected chickens were used to ensure that the virus inoculum administered to the ducks produced the disease in chickens, the susceptible hosts. Ducks were killed on day 1, 4, 8 and 14 post-infection. Postmortem examination was done with six tissues (liver, spleen, lung, caecal tonsils, kidneys and brain) being collected from each bird. The tissues were preserved in 10% neutral formalin for 24 h. They were then transferred to 70% ethanol for histology and immunohistochemical staining. Airsacculitis, necrotic splenic foci, congested intestines, lymphoid depleted caecal tonsils and focal infiltrations by mononuclear cells were the main pathological lesions in infected ducks. Over 28.9% of the infected ducks had Newcastle disease viral nucleoprotein in macrophage-like large mononuclear cells in the caecal tonsils and kidney tubular epithelium. The viral antigens were located in the cytoplasm and nucleolus of the cells. The other organs had no detectable viral antigens. This study shows that the kidneys and caecal tonsils are the likely predilection sites for the virus in ducks. They thus need to be considered as diagnostic indicators for the viral carriage in ducks
GICHOVI PROFMBOGOHSTEPHEN. "Lucy Ngare's M.Sc. Research and Thesis on the Potential Socio-economic Effects of Adopting Herbicide-coated Maize Seed as a Technology in Striga Weed Control: Student graduated with a Master's Degree in 2004.". In: Nairobi, Catholic University in Eastern Africa, 2002. D.M.Matheka,T.N kiama; 2004. Abstract
Department of Pathology, University of Toronto, Ontario, Canada. Red blood cells and lysate products (erythrolysate) are observed consistently in lymph draining acute and chronic inflammatory reactions and from tissues subjected to trauma or surgical procedures. Using hemoglobin as a marker for erythrolysate, we have measured hemoglobin in lymph up to the 10(-6) M range in a number of pathophysiological states. Data demonstrate that erythrolysate alters the pumping characteristics of lymphatic vessels. To test the effects of erythrolysate on lymphatic pumping, bovine lymphatics were suspended in an organ bath preparation with the vessels cannulated at both inflow and outflow ends. By raising the heights of the Krebs reservoir and the outflow catheters appropriately, a transmural pressure that stimulated pumping activity could be applied to the vessels. With a fixed transmural pressure of 6 cm H2O applied to the ducts, sheep erythrolysate depressed pumping activity between 40% and 100%, with dilutions containing between 10(-8) and 10(-5) M hemoglobin. Although the active principle in the red blood cells has not been characterized, evidence from precipitation purification experiments suggests that hemoglobin is an important component. Once suppressed, pumping could be restored in many but not all vessels (often to control levels) by elevating the distending pressure above 6 cm H2O. The relation between transmural pressure and fluid pumping is expressed as a bell-shaped curve, with pumping increasing up to a peak pressure (usually 8 cm H2O) and declining at pressures above this level. By comparing pressure/flow curves, we were able to ascertain that hemoglobin shifted the lymphatic function curve to the right and, on average, reduced the maximum pumping capability of the vessels. We speculate that the presence of erythrolysate/hemoglobin in lymph may modulate the ability of lymphatic vessels to drain liquid and protein from the tissue spaces.
O. PROFNDINYA-ACHOLAJ. "Lubwama SW, Plummer FA, Ndinya-Achola J, Nsanze H, Namaara W, D'Costa LJ, Ronald AR.Isolation and identification of Haemophilus ducreyi in a clinical laboratory.J Med Microbiol. 1986 Sep;22(2):175-8.". In: J Med Microbiol. 1986 Sep;22(2):175-8. IBIMA Publishing; 1986. Abstract
Routine procedures used to isolate Haemophilus ducreyi in a busy laboratory are reported. Identification was based on colony morphology and nutritional and biochemical properties of 120 fresh isolates of H. ducreyi. These isolates grew very well on Gonococcal Agar and Mueller-Hinton Agar incubated at 34 degrees C in candle extinction jars containing moistened filter paper. Colonies varied in size, giving a polymorphic appearance. They were smooth, dome-shaped, and buff-yellow to grey in colour, and measured 2 mm in diameter. They could be pushed intact across the agar surface. By microscopic examination of gram-stained smears the isolates were gram-negative coccobacilli arranged in short chains, clumps or whorls and occasionally in typical "rail track" arrangements. Individual bacteria showed bipolar staining. Colonies autoagglutinated in saline. All strains were catalase-negative and did not produce indole or H2S. They were oxidase- and beta-lactamase positive and required X but not V factor for growth. Now that reliable techniques have been developed and characteristics established it is possible for most clinical laboratories to isolate and identify this organism from most patients with chancroid.
O. PROFNDINYA-ACHOLAJ. "Lubwama SW, Plummer F, Ndinya-Achola JO, Nsanze H, Namaara W, D'Costa LJ. Isolation and identification of H. ducreyi in a clinical laboratory. J. Med. Microbiol. 22: 175 - 178, 1986.". In: J. Med. Microbiol. 22: 175 - 178, 1986. IBIMA Publishing; 1986. Abstract
OBJECTIVE–To determine the efficacy of the nonoxynol 9 contraceptive sponge in preventing sexual acquisition of the human immunodeficiency virus (HIV). DESIGN–Prospective, randomized placebo-controlled trial. SETTING–Research clinic for prostitutes in Nairobi, Kenya. PATIENTS AND INTERVENTIONS–One hundred thirty-eight HIV-seronegative women were enrolled, of whom 74 were assigned to nonoxynol 9 sponge use and 64 to placebo use. These two groups did not significantly differ with respect to demographic characteristics, sexual practices, or prevalence of genital infections at enrollment, except for a lower number of sex partners per week and a higher initial prevalence of genital ulcers among women assigned to nonoxynol 9 sponge use. Among the 116 women who returned for follow-up, the mean durations of follow-up were 14 and 17 months for the two groups, respectively. MAIN OUTCOME MEASURE–HIV seroconversion. RESULTS–Nonoxynol 9 sponge use was associated with an increased frequency of genital ulcers (relative risk [RR], 3.3; P less than .0001) and vulvitis (RR, 3.3; P less than .0001) and a reduced risk of gonococcal cervicitis (RR, 0.4; P less than .0001). Twenty-seven (45%) of 60 women in the nonoxynol 9 sponge group and 20 (36%) of 56 women in the placebo group developed HIV antibodies. The hazard ratio for the association between nonoxynol 9 sponge use and HIV seroconversion was 1.7 (95% confidence interval [CI], 0.9 to 3.0). Using multivariate analysis to control for the presence of genital ulcers at enrollment, the adjusted hazard ratio for the association between nonoxynol 9 sponge use and seroconversion was 1.6 (95% CI, 0.8 to 2.8). CONCLUSIONS–Genital ulcers and vulvitis occurred with increased frequency in nonoxynol 9 sponge users. We were unable to demonstrate that nonoxynol 9 sponge use was effective in reducing the risk of HIV infection among highly exposed women.
O. PROFNDINYA-ACHOLAJ. "Lubwama SW, Nsanze H, Plummer F, Ndinya-Achola J, Ronald AR, D'Costa LJ, Naamara W, Maitha G.Laboratory diagnosis of chancroid in Nairobi, Kenya. East Afr Med J. 1986 Jun;63(6):389-94.". In: East Afr Med J. 1986 Jun;63(6):389-94. IBIMA Publishing; 1986. Abstract
Routine procedures used to isolate Haemophilus ducreyi in a busy laboratory are reported. Identification was based on colony morphology and nutritional and biochemical properties of 120 fresh isolates of H. ducreyi. These isolates grew very well on Gonococcal Agar and Mueller-Hinton Agar incubated at 34 degrees C in candle extinction jars containing moistened filter paper. Colonies varied in size, giving a polymorphic appearance. They were smooth, dome-shaped, and buff-yellow to grey in colour, and measured 2 mm in diameter. They could be pushed intact across the agar surface. By microscopic examination of gram-stained smears the isolates were gram-negative coccobacilli arranged in short chains, clumps or whorls and occasionally in typical "rail track" arrangements. Individual bacteria showed bipolar staining. Colonies autoagglutinated in saline. All strains were catalase-negative and did not produce indole or H2S. They were oxidase- and beta-lactamase positive and required X but not V factor for growth. Now that reliable techniques have been developed and characteristics established it is possible for most clinical laboratories to isolate and identify this organism from most patients with chancroid.
Kimeu M. "The LRC: Wholistic Green Building Design In Nairobi.". In: Green Building Training in Kampala organised by UN Habitat. Kampala; 2014.
Kimeu M. "The LRC: Wholistic Green Building Design In Nairobi.". In: Green Building Training in Nairobi. Apollo Centre in Westlands, Nairobi.; 2014.
M.Kimeu. "The LRC: Holistic Green building design finally in Nairobi.". In: East African regional Workshop East African regional Workshop . ADD building, University of Nairobi; 2013.
M.Kimeu. "The LRC: Green Building Design Finally in Nairobi." Buildesign magazine. 2013;(5):Pages 06-13.
Kimeu M. "The LRC at The Catholic University of Eastern Africa.". In: UN-Habitat Conference on Financing Green Building in Africa. Strathmore University; 2013.
M.Kimeu. "LRC at CUEA: Wholistic Green Building Design Finally Arrives in Nairobi." The Architect magazine. 2013;(2):12-25.
S. R, M. P, V. O. "LP Fracturing: A Review on Waterless Fracturing Technology in Unconventional Reservoir." Journal of Scientific and Engineering Research (JSAER). 2021;8(1):48-54.
O. DRBUKACHIFREDERICK. "Lown B, Bukachi F, Xavier R. Health information in the developing world. Lancet. 1998 Oct;352 Suppl 2:SII34-8.". In: East Afr Med J. 2001 Jul;78(7 Suppl):S43-7. UN-HABITAT; 1998. Abstract

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OBJECTIVE: To assess the clinical outcome of successful percutaneous transluminal coronary angioplasty (PTCA) in patients with poor ventricular function. METHODS: Analysis of angiographic, echocardiographic and clinical records of patients with severe LV dysfunction who underwent PTCA from January 1, 1995 to December 31, 1997 was undertaken. Forty-one patients aged 63+/-10 years, 36 men, all with significant coronary artery disease and impaired LV function (fractional shortening, FS<or=20%) were identified. Patients' data before and after angioplasty were analyzed. RESULTS: Post PTCA: angiographic success was 95.2%. Major complications occurred in 19.5% and hospital mortality was 2.7%. At 6 months after PTCA:LV fractional shortening (FS) increased from 15.9+/-3.4% to 19.6+/-6.6%

ODHIAMBO DRALFRED, BYAKIKA DRBESSIE, GACHIE DRANDREW, OLIECH PROFJOSEPH. "Lower urinary tract symptoms (LUTS) as the initial presentation for colorectal malignancy.". Abstractluts_presentation.pdf

The presenting features of colorectal carcinoma in the African patient are not different from reports worldwide although the majority present late with features of advanced disease (1). Increasing frequency of diagnosis may be attributed the twin effects of changing diets and easier access particularly by the elite population to modern diagnostic imaging tools.
A 72 year old African male presented to a private practitioner with a 2 week history hematuria, frequency, nocturia and subsequently a 2 day history of retention of urine (AUR). Imaging studies demonstrated a tumour mass invading the roof of the urinary bladder. Following surgical extirpation the mass was subjected to histopathological evaluation.

Lehman DA, Chung MH, Mabuka JM, John-Stewart GC, Kiarie J, John Kinuthia, James N Kiarie, Overbaugh J. "Lower risk of resistance after short-course HAART compared with zidovudine/single-dose nevirapine used for prevention of HIV-1 mother-to-child transmission." J. Acquir. Immune Defic. Syndr.. 2009;51(5):522-9. Abstract

Antiretroviral resistance after short-course regimens used to prevent mother-to-child transmission has consequences for later treatment. Directly comparing the prevalence of resistance after short-course regimens of highly active antiretroviral therapy (HAART) and zidovudine plus single-dose nevirapine (ZDV/sdNVP) will provide critical information when assessing the relative merits of these antiretroviral interventions.

Ating’a JEO, KO A. "Lower limb amputations at the Kenyatta National Hospital, Nairobi. East Afr Med J. 2007 Mar;84(3):121-6.". In: Occasional Publication Number 3 2003. pp 21-32. E Afr Med J; 2007. Abstract

OBJECTIVE: To determine the causes and pattern of lower limb amputations at the Kenyatta National Hospital. DESIGN: Descriptive prospective study. SETTING: The Kenyatta National Hospital between July 2003 and June 2004. RESULTS: A total of 77 lower limb amputations (LLA) were performed on 74 patients. The age ranged from seven months to 96 years (mean 44.8 +/- 22.5). Forty six patients (62.1%) were male. Majority of the patients (89.1%) had primary or no formal education, forty one (55.4%) were unemployed, with 39% self employed in the informal sector. Peripheral vascular diseases were the main indication for LLA (55.3%), 13 patients (17.5%) due to diabetes-related gangrene. Eighteen patients (24.3%) had tumours, mainly osteogenic sarcoma (16.2%), while trauma accounted for 18.9%. Forty two (55%) of the amputations were above-the-knee, 24 (31%) below-the-knee, four (5%) hip disarticulations and seven (9%) were foot amputations. CONCLUSION: This study found peripheral vascular diseases unrelated to diabetes to be the main indication for lower limb amputations at Kenyatta National Hospital contrary to previous institutional and loco-regional studies which report trauma as the leading cause. Further investigation into vascular causes is therefore recommended.

Mutuma M, Muthomi JW, Stasiewicz M. "Low-cost Optical Sorting to Remove Mycotoxins from Maize in Local Kenyan Mills.". In: Nairobi Innovation Week 2016. University of Nairobi, Kenya; 2016.
Olwal TO, Van Wyk MA, Van Wyk MA, Odhiambo M, Van Wyk BJ. "Low Variance Timing Recovery in Turbo Receivers."; 2006.
N. DRNJENGAHELLEN. "Low Pressure and Salt Effect on the Ethanol-Water Vapour-Liquid Equilibrium.". In: Ph.D. Thesis University of Wales. Elsevier; 1991.
O PROFBWIBONIMROD. "Low plasma vitamin B-12 in Kenyan school children is highly prevalent and improved by supplemental animal source foods. J Nutr . 2007 Mar; 137 ( 3 ): 676-82 . PMID: 17311959 [PubMed - indexed for MEDLINE] McLean ED, Allen LH, Neumann CG, Peerson JM, Siekm.". In: J Nutr . 2007 Mar; 137 ( 3 ): 676-82 . Anim. Hlth. Prod. Afr. 2008; 2007. Abstractlow_plasma_vitamin_b-12.pdf

Department of Nutritional Biology, University of California, Davis, CA 95616, USA.

The high prevalence of vitamin B-12 deficiency in many regions of the world is becoming recognized as a widespread public health problem, but it is not known to what extent this deficiency results from a low intake of the vitamin or from its malabsorption from food. In rural Kenya, where a previous study identified a high prevalence of inadequate vitamin B-12 intakes, this study examined whether plasma vitamin B-12 concentrations were associated with dietary sources of the vitamin at baseline and could be increased by supplementation with animal source foods (ASF). The 4 experimental groups in 503 school children were: 1) control (no food provided); 2) githeri (a maize and bean staple with added oil); 3) githeri + meat (githeri + minced beef); or 4) githeri + milk (githeri + milk). Feedings were isocaloric. Dietary data were collected at baseline, and biochemical data at baseline and after 1 and 2 y of feeding. Baseline plasma vitamin B-12 concentration was 193.6 +/- 105.3 pmol/L and correlated with % energy from ASF (r = 0.308, P < 0.001). The odds ratio for low plasma vitamin B-12 (<148 pmol/L), which occurred in 40% of children, was 6.28 [95% CI: 3.07-12.82] for the lowest vs. highest ASF intake tertile (P < 0.001). Feeding ASF (meat or milk) greatly reduced the prevalence of low plasma vitamin B-12 (P < 0.001). The high prevalence of low plasma vitamin B-12 concentrations in these children is predicted by a low intake of ASF, and supplemental ASF improves vitamin B-12 status.

PMID: 17311959 [PubMed - indexed for MEDLINE]

O. J. Olwendo, T. Baluku, P. Baki, P.J. Cilliers, C. Mito, and Doherty P. "Low latitude ionospheric scintillation and zonal irregularity drifts observed with GPS-SCINDA System and closely spaced VHF receivers in Kenya." Advances in Space Research. 2013;51(9):1715-1726.
JOAB PROFBWAYOJOB. "Low dose of erythromycin in the treatment of chancroi. Kimani J, Maclean I, Anzala A, Bwayo J.J. East-AfricanMedical Journal. 1995 Oct; 72(10): 645-8.". In: East-AfricanMedical Journal. 1995 Oct; 72(10): 645-8. Asian Economic and Social Society; 1995. Abstract
The factors responsible for the explosive spread of human immunodeficiency virus type 1 (HIV-1) in sub-Saharan Africa continue to be identified and debated. One of the most controversial factors has been male circumcision. This cross-sectional study was conducted to measure the association between circumcision status and infection with HIV-1 among men with genital ulcer disease. Eight hundred and ten men participated in the study, of whom 190 (23%) were HIV-1-positive. A logistic regression model adjusted for behavioral and historical showed that HIV-1 positivity was independently associated with being uncircumcised (adjusted odds ratio [OR], 4.8; 95% confidence interval [CI], 3.3-7.2) and with a history of urethral discharge (adjusted OR, 2.0; 95% CI, 1.4-2.8). This association could not be explained by measures of sexual exposure to HIV-1 among this population. Male circumcision should be considered as an intervention strategy for AIDS control.
JOAB PROFBWAYOJOB, O. PROFANZALAAGGREY. "Low dose erythromycin regimen for the treatment of chancroid. Kimani J; Bwayo JJ; Anzala AO; MacLean I; Mwatha A; Choudri SH; Plummer FA; Ronald AR. East Afr Med J. 1995 Oct;72(10):645-8.". In: East Afr Med J. 1995 Oct;72(10):645-8. Asian Economic and Social Society; 1995. Abstract
The factors responsible for the explosive spread of human immunodeficiency virus type 1 (HIV-1) in sub-Saharan Africa continue to be identified and debated. One of the most controversial factors has been male circumcision. This cross-sectional study was conducted to measure the association between circumcision status and infection with HIV-1 among men with genital ulcer disease. Eight hundred and ten men participated in the study, of whom 190 (23%) were HIV-1-positive. A logistic regression model adjusted for behavioral and historical showed that HIV-1 positivity was independently associated with being uncircumcised (adjusted odds ratio [OR], 4.8; 95% confidence interval [CI], 3.3-7.2) and with a history of urethral discharge (adjusted OR, 2.0; 95% CI, 1.4-2.8). This association could not be explained by measures of sexual exposure to HIV-1 among this population. Male circumcision should be considered as an intervention strategy for AIDS control.
JOAB PROFBWAYOJOB, O. PROFANZALAAGGREY. "Low dose erythromycin regimen for the treatment of chancroid. Kimani J; Bwayo JJ; Anzala AO; MacLean I; Mwatha A; Choudri SH; Plummer FA; Ronald AR. East Afr Med J. 1995 Oct;72(10):645-8.". In: East Afr Med J. 1995 Oct;72(10):645-8. John Benjamins Publishing Company; 1995. Abstract

BACKGROUND: There is indirect evidence that HIV-1 exposure does not inevitably lead to persistent infection. Heterogeneity in susceptibility to infection could be due to protective immunity. The objective of this study was to find out whether in highly HIV-1-exposed populations some individuals are resistant to infection. METHODS: We did an observational cohort study of incident HIV-1 infection-among 424 initially HIV-1-seronegative prostitutes in Nairobi, Kenya, between 1985 and 1994. 239 women seroconverted to HIV-1 during the study period. Exponential, Weibull, and mixture survival models were used to examine the effect of the duration of follow-up on incidence of HIV-1 infection. The influence of the duration of exposure to HIV-1 through prostitution on seroconversion risk was examined by Cox proportional hazards modelling, with control for other known or suspected risk factors for incident HIV-1 infection. HIV-1 PCR with env, nef, and vif gene primers was done on 43 persistently seronegative prostitutes who remained seronegative after 3 or more years of follow-up. FINDINGS: Modelling of the time to HIV-1 seroconversion showed that the incidence of HIV-1 seroconversion decreased with increasing duration of exposure, which indicates that there is heterogeneity in HIV-1 susceptibility or acquired immunity to HIV-1. Each weighted year of exposure through prostitution resulted in a 1.2-fold reduction in HIV-1 seroconversion risk (hazard ratio 0.83 [95% CI 0.79-0.88], p < 0.0001). Analyses of epidemiological and laboratory data, show that persistent seronegativity is not explained by seronegative HIV-1 infection or by differences in risk factors for HIV-1 infection such as safer sexual behaviours or the incidence of other sexually transmitted infections. Interpretation: We conclude that a small proportion of highly exposed individuals, who may have natural protective immunity to HIV-1, are resistant to HIV-1. PIP: A cohort study conducted in 1985-94 among 424 prostitutes from Nairobi, Kenya, who were initially human immunodeficiency virus (HIV)-1 seronegative, tended to provide support for the observation that some individuals in highly exposed populations may be resistant to infection. During the 10-year study period, 239 of these women seroconverted. The overall HIV-1 incidence was 42/100 person-years. After the first 2 years of follow up, in which the majority of seroconversions occurred, HIV-1 prevalence reached a plateau and then began a steep decline. To determine whether the risk of HIV-1 infection declined over time as a result of the selection of resistance, incidence rates among women with less than 3 years' versus more than 3 years' duration of prostitution were compared for 1989-93. An increasing protective effect for each seronegative year of exposure was observed. The estimated cumulative protective effect for women practicing prostitution from 1984-93 and remaining seronegative, compared to women who entered prostitution in 1994, was over 100-fold. To rule out the possibility that the decrease in seroconversion with duration of exposure reflected differences in sexual behavior or immunity to sexually transmitted diseases that facilitate HIV transmission, Cox proportional hazards modelling was performed. The weighted duration of prostitution was independently associated with a decreased risk of seroconversion. Each weighted year of exposure resulted in a 1.2-fold decrease in risk. Women who seroconverted were more likely to report 1 or more regular partners and to use condoms with these partners than their counterparts who remained seronegative. Elucidation of the protective mechanisms and the factors mediating the development of immunity against HIV-1 could be important to HIV-1 vaccine research.

Magoha GAO. "Low dose clomiphane citrate therapy in oligospermic men with idiopathic primary infertility. ." Nairobi Journal of Medicine.. 1988;15:42-45. Abstract

Eighty-one Nigerian patients with testicular torsion presented at the Urology Unit of the Lagos University Teaching Hospital between May 1983 and May 1985. The majority (62%) were young adults of 21 years and above with a mean of 21.3 +/- 5.2 years. Fifty-four testes found to be clinically non viable at exploration were excised and microscopically confirmed as haemorrhagic infarction due to the torsion. Twenty-six clinically viable testes at exploration were salvaged giving a low salvage rate of 32%. This appears to be due to delayed or mistaken diagnosis by the first doctor to see the patient as a result of low index of suspicion. The clinical importance of high index of suspicion for testicular torsion in all patients with testicular pain of recent onset irrespective of age is emphasized in this locality.

Onwonga RN, Lelei JJ, Freyer B, Friedel JK, Mwonga SM, Wandhawa P. "Low Cost Technologies for Enhancing N and P Availability and Maize (Zea mays L.) Performance on Acid Soils." World Journal of Agricultural Sciences. 2008;4(s):862-873.onwonga_et_al_2008_low_cost_technologies_for_enhancing_n_and_p_availability_2.pdf
Birech Z, Ondieki AM, Opati RII, Mwangi PW. "Low cost Raman sample substrates from conductive silver paint smear for Raman spectroscopic screening of metabolic diseases in whole blood.". 2020;108:103063. AbstractWebsite

This work reports on a low cost, simple to prepare and chemically stable Raman substrates based on conductive silver paint smear. The substrates were characterized Raman spectroscopically and were found to be chemically stable within the first seven days when kept at room temperature as the spectroscopic profiles were unchanged. The substrates also suppressed the background signals emanating from glass centered around 750 cm−1 and 1370 cm−1 seen with 785 nm excitation and had negligible influence on Raman spectral profiles of rat’s blood samples applied onto them. The Raman spectral profiles of blood samples applied onto the substrates were found to be enhanced by a factor of 1.7 compared to those of thick blood smears on a clean microscope glass slide. The increased local field between the gaps formed by adjacent micron-sized silver solids in the paint smear were attributed to the observed intense signals observed from the blood samples applied onto them. The substrates were tried on Raman spectroscopic differentiation between blood from obese and normal; diabetic and normal Sprague Dawley rats. The prominent bands associated with fructose (638 and 812 cm−1), glucose (1127 cm−1) and branched chain amino acids (1033, 1217 and 1318 cm−1) were observed to vary in terms of intensity between the un-healthy (obese and diabetic) and healthy (normal) rats. The results reported here on the use of the easy to prepare, low cost Raman substrates have the potential of making surface-enhanced Raman spectroscopy accessible to less resourced laboratories in developing countries. The substrates can be applied in rapid Raman spectroscopic screening of various metabolic diseases.

N PROFMUSOKERACHEL. "Low birthweight. East Afr Med J . 1986 Feb; 63 ( 2 ): 89-90 . No abstract available. PMID: 3720609 [PubMed - indexed for MEDLINE] Musoke RN.". In: East Afr Med J . 1986 Feb; 63 ( 2 ): 89-90 . Far East Journal of Theoretical Statistics; 1986. Abstract
No abstract available.
Musoke RN. "Low birthweight.". 1986.
Wasunna A, Mohammed K. "Low birthweight babies: socio-demographic and obstetric characteristics of adolescent mothers at Kenyatta National Hospital, Nairobi." East Afr Med J. 2002;79(10):543-6. Abstract

To compare some socio-demographic and obstetric factors between adolescent mothers (aged below 20 years) and older mothers of low birthweight (birthweight < 2000 gm) babies.

PARK JEONGKYUNG. "Love and War in Alex La Guma’s “Thang’s Bicycle." Korean Association of African Studies. 2019;57:161-182.
Lodge, D. M., Rosenthal1, S. K., Mavuti, Muohi, W., Ochieng, P., Stevens, S. S., Mungai BN, Mkoji GM. "Louisiana crayfish (Procambarus clarkii) (Crustacea: Cambaridae) in Kenyan ponds: non-target effects of a potential biological control agent for schistosomiasis." African Journal of Aquatic Science. 2005;30(2):119-124.
PROF. MAVUTI KENNETHM. "Louisiana crayfish (Procambarus clarkii) (Crustacea: Cambaridae) in Kenyan ponds: non-target effects of a potential biological control agent for schistosomiasis.". In: African Journal of Aquatic Science 2005, 30(2). Vaccine 26:2788- 2795; 2005. Abstract
Normal 0 false false false MicrosoftInternetExplorer4 st1:*{behavior:url(#ieooui) } /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-parent:""; mso-padding-alt:0in 5.4pt 0in 5.4pt; mso-para-margin:0in; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Times New Roman"; mso-ansi-language:#0400; mso-fareast-language:#0400; mso-bidi-language:#0400;} Macrophytes have been shown to perform important ecological roles in Lake Naivasha. Consequently, various studies regarding the impact of biotic factors on the macrophytes have been advanced but related studies on environmental parameters have lagged behind. In an attempt to address this gap, sampling on floating species and submergents was carried out in eight sampling sites in 2003 to investigate how they were influenced by a set of environmental factors. Soil texture (sandy sediments; P < 0.05, regression coefficient = - 0.749) and wind were the most important environmental parameters influencing the distribution and abundance of floating macrophytes. Combination of soil texture and lake-bed slope explained the most (86.3%) variation encountered in the submergents. Continuous translocation of the floating dominant water hyacinth to the western parts by wind has led to displacement of the submergents from those areas. In view of these findings, the maintenance and preservation of the steep Crescent Lake basin whose substratum is dominated by sand thus hosting most submergents remain important, if the whole functional purpose of the macrophytes is to be sustained.
Gachene, C K K; Mureithi JG. Lost and reclaimed: A case study of gully rehabilitation in central Kenya highlands using low-cost measures.; 2004. Abstract

Gully control and reclamation activities using low-cost measures were carried out in early March 2001 at Gatanga division, Kenya. The study area was selected on the basis of previous work carried out in farmers fields by the Legume Research Network Project (LRNP). The project’s main objective is to introduce green manure legume species that perform well in different agro ecological zones of Kenya mainly for the purpose of soil fertility improvement and erosion control in smallhold farms. Area studied is characterized by a mean annual rainfall of about 1100 mm with a bimodal distribution, deep red soils, steep slopes and intensive landuse. Field activities were carried out in one of the farms which had literally been abandoned due to gully erosion. The length of the gully was 130 m with an average width and depth of 1.62 and 1.4 m, respectively. Work involved planting of grasses (mainly Brachira humidocola) and mucuna (Mucuna pruriens) on the floor and sides of the gully. In addition ‘macro-contour lines’ were constructed in the farm which involved planting lines of mucuna, sesbania (Sesbania sesban) and napier grass (Pennisetum purpureum) along the terrace embankments. Through photographs taken over a 3 year period, evidence is given to show that the gully has completely healed and that the farm has been brought back to productivity.

Graham SM, Raboud J MCRSJN-AMOBAMWJKJ. "Loss to Follow-Up as a Competing Risk in an Observational Study of HIV-1 Incidence." PLoS ONE. 2013;8(3):e59480.abstract.pdf
Graham SM, Raboud J MCRSJN-AMOBAMWJKJ. "Loss to Follow-Up as a Competing Risk in an Observational Study of HIV-1 Incidence." PLoS One. . 2013;8(3):e59480.
Spranger J, Osterhoff M, Reimann M, Möhlig M, Ristow M, Francis MK, Cristofalo V, Hammes H-P, Smith G, Boulton M. "Loss of the antiangiogenic pigment epithelium-derived factor in patients with angiogenic eye disease." Diabetes. 2001;50:2641-2645. AbstractWebsite
n/a
"Loss of Material Heritage in Kenya." International Journal of Arts and Commerce . 2013;2(9):77-92.
Obondo A, editor Ndetei, D.M., Rono R, Ngare D. "Loss and Bereavement Therapies."; 2006.
PAUL PROFBAKI. "Lorentz- Non-invariance Effect on Flavour Oscillations, General Relativity and Gravitation.". In: Vol. 35, 891(2003). Heinrich Boll Foundation.; 2003.
N PROFOGOLAELIJAHS. "LORE W; MUITA A.K; OGOLA E.N; Efficacy and tolerability of Enalapril hydrochlorothiazide combination as first line therapy in essential arterial hypertension in Blacks, a Clinical Trial Kenya. East Africa .Med J.69: 18, 1992.". In: East Africa .Med J.69: 18, 1992. Kisipan, M.L.; 1992. Abstract

Sixty patients with Diastolic Blood Pressure (DBP) of 100-110mmHg matched for age, sex and Bp levels were randomly assigned to propranolol 80mg daily or Hydroflumethiazide (HFM) 50mg daily. HFM causes a significant reduction in systolic blood pressure (SBP) and DBP within 4 weeks compared both with baseline and propranolol (SBP 143.7 +/- 12.3 vs 158.1 +/- 10.9mmHg, P < 0.05; DBP 92.0 +/- 4.5 vs 102.4 +/- 5.1mmHg, P < 0.05), (SBP 143.7 +/- 12.2 vs 152 +/- 11.0mmHg P < 0.05; DBP 92.0 +/- 4.5 vs 101.1 +/- 6.1mmHg, P < 0.05), respectively. Propranolol produced no significant difference from the baseline at 4 weeks (SBP 152.0 +/- H.0 vs 154.1 +/- 11.5mmHg NS; DBP 101.1 +/- 6.1 vs 102.2 +/- 5.6mmHg, NS). Reduction in BP by HFM was maintained after 8 and 12 weeks with further reduction but which did not achieve statistical significance. Increased dose of propranolol (160mg daily) after 4 weeks caused significant reduction in BP by 8 week (SBP 146.8 +/- 11.8 vs 152.0 +/- 11.0mmHg, P < 0.05; DBP 95.9 +/- 4.4 vs 101.1 +/- 6.1mmHg P < 0.05), which was maintained upto 12 weeks. The values however remained higher than in the HFM group. More patients in the HFM group achieved target BP (< 140/90), SBP 53.8% vs 29.6% P < 0.05, DBP 69.2% vs 14.8% P < 0.01. Incidence of side effects was similar and will be discussed. Thiazides are superior to B'blockers as initial monotherapy in black hypertensives.

N PROFOGOLAELIJAHS. "LORE W, OGOLA E.N; Medico social Characteristics of Cardiac patients in Obstetrical Unit Of Kenyatta National Hospital. A preliminary report of an on-going prospective study. J.Obs. Gynaecol. East and Central Africa 6:83,1987.". In: J.Obs. Gynaecol. East and Central Africa 6:83,1987. Kisipan, M.L.; 1987. Abstract
The study aimed at evaluating tolerability and efficacy of the combination enalapril 20 mg with hydrochlorothiazide 12.5 mg (co-renitec) as first line therapy in black patients with mild to moderate primary hypertension. Fifty patients completed a twelve weeks of open clinical study preceded by two weeks of washout period. They were evaluated every four weeks and haematological, biochemical urine microscopy and electrocardiographic tests were undertaken before the start and after the completion of study. Pre-treatment values of mean systolic blood pressure (SBP) and diastolic blood pressure (DBP) were 172.16 mm hg (+/- 20.41) and 104.38 mm hg (+/- 7.339) respectively. The usual daily dosage was one tablet which was increased to two after eight weeks in case the DBP was not normalized, i.e. less than or equal to 95 mm hg. In 44 (88%) patients, the DBP was normalised at the end of the study period; three patients (6%) were resistant to treatment and another three (6%) exhibited labile response to the treatment. Clinical tolerance was considered to be very good with only five episodes of headache, backache and anxiety, probably not related to the test drug. Biological tolerance was excellent: there was no change in the haematologic parameters; there was a decrease of 5% in mean blood urea, of 9% in the mean serum creatinine and of 4% in the mean serum uric acid and a 5% increase in plasma potassium from 3.99 to 4.28 mmol/l.(ABSTRACT TRUNCATED AT 250 WORDS)
N PROFOGOLAELIJAHS, OTIENO PROFOGUTUELLY. "Lore W, Muita AK, Ogola ES.The efficacy and tolerability of enalapril–hydrochlorothiazide combination as a first line therapy in black patients with mild to moderate arterial hypertension: a clinical study in Kenya.East Afr Med J. 1992 Jan;69(1):18-21.". In: East Afr Med J. 1992 Jan;69(1):18-21. Kisipan, M.L.; 1992. Abstract
The study aimed at evaluating tolerability and efficacy of the combination enalapril 20 mg with hydrochlorothiazide 12.5 mg (co-renitec) as first line therapy in black patients with mild to moderate primary hypertension. Fifty patients completed a twelve weeks of open clinical study preceded by two weeks of washout period. They were evaluated every four weeks and haematological, biochemical urine microscopy and electrocardiographic tests were undertaken before the start and after the completion of study. Pre-treatment values of mean systolic blood pressure (SBP) and diastolic blood pressure (DBP) were 172.16 mm hg (+/- 20.41) and 104.38 mm hg (+/- 7.339) respectively. The usual daily dosage was one tablet which was increased to two after eight weeks in case the DBP was not normalized, i.e. less than or equal to 95 mm hg. In 44 (88%) patients, the DBP was normalised at the end of the study period; three patients (6%) were resistant to treatment and another three (6%) exhibited labile response to the treatment. Clinical tolerance was considered to be very good with only five episodes of headache, backache and anxiety, probably not related to the test drug. Biological tolerance was excellent: there was no change in the haematologic parameters; there was a decrease of 5% in mean blood urea, of 9% in the mean serum creatinine and of 4% in the mean serum uric acid and a 5% increase in plasma potassium from 3.99 to 4.28 mmol/l.(ABSTRACT TRUNCATED AT 250 WORDS)
N PROFOGOLAELIJAHS, OTIENO PROFOGUTUELLY. "Lore W, Muita AK, Ogola ES.The efficacy and tolerability of enalapril–hydrochlorothiazide combination as a first line therapy in black patients with mild to moderate arterial hypertension: a clinical study in Kenya.East Afr Med J. 1992 Jan;69(1):18-21.". In: East Afr Med J. 1992 Jan;69(1):18-21. Journal of British Ceramic Transactions, 99 [5], 206-211.; 1992. Abstract
The study aimed at evaluating tolerability and efficacy of the combination enalapril 20 mg with hydrochlorothiazide 12.5 mg (co-renitec) as first line therapy in black patients with mild to moderate primary hypertension. Fifty patients completed a twelve weeks of open clinical study preceded by two weeks of washout period. They were evaluated every four weeks and haematological, biochemical urine microscopy and electrocardiographic tests were undertaken before the start and after the completion of study. Pre-treatment values of mean systolic blood pressure (SBP) and diastolic blood pressure (DBP) were 172.16 mm hg (+/- 20.41) and 104.38 mm hg (+/- 7.339) respectively. The usual daily dosage was one tablet which was increased to two after eight weeks in case the DBP was not normalized, i.e. less than or equal to 95 mm hg. In 44 (88%) patients, the DBP was normalised at the end of the study period; three patients (6%) were resistant to treatment and another three (6%) exhibited labile response to the treatment. Clinical tolerance was considered to be very good with only five episodes of headache, backache and anxiety, probably not related to the test drug. Biological tolerance was excellent: there was no change in the haematologic parameters; there was a decrease of 5% in mean blood urea, of 9% in the mean serum creatinine and of 4% in the mean serum uric acid and a 5% increase in plasma potassium from 3.99 to 4.28 mmol/l.(ABSTRACT TRUNCATED AT 250 WORDS)
Maina DSM, Gatere G. "Looking, Learning & Seeing: the role of design in developing exhibition and display in Nairobi." Africa Habitat Review. 2015;vol. 9(No. 9):805-822.
Maina E. "Looking to the study of genes for cancer diagnoses and Treatment." Your Doctor Magazine. 2012;4(1):5-6.
wa Mutiso K. "Looking at each other: The Origin of Negative Ethnicity." Mwanga wa Lugha . 2017;2(2):63-80 .
R.S. O. "A Look at Climate Change and Human Mobility in Africa -,." paper presents at Cespi Workshop,Italian Ministry of Foreign Affairs; 2009. Abstract
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Susan S Imbahale, Krijn P Paaijmans, Wolfgang R Mukabana, Ron van Lammeren, Githeko AK, Takken W. "A longitudinal study on Anopheles mosquito larval abundance in distinct geographical and environmental settings in western Kenya. ." Malaria Journal. 2011;10:81.
ARIMI PROFMUTWIRIS, A MRKABURIAHF. "A longitudinal study of milk somatic cell counts and bacterial culture from cows on smallholder dairy farms in Kiambu District, Kenya.". In: journal. GIGA German Institute of Global and Area Studies, Hamburg, July 2009; 1996. Abstract
As part of an integrated study on health and production of dairy cattle on smallholder farms in central Average milk yield was low (5.8 kg/day; median = 5kg/day) and lactation length was long (20 months). Clinical mastitis risk was low (1% per month). Somatic cell counts (SCC) were high (median = 620 x 103); the previously suggested threshold of 300 000 cells/ml would classify 71% of quarters as positive for subclinical mastitis. Bacteria were commonly isolated, with S. aureus as the most common pathogen isolated (22.1% of all samples). Infections with mastitis pathogens, cow-age and milk yield were associated with increases in SCC. However, S. aureus was the only mastitis pathogen associated with decreased milk yield. Few specific mastitis control measures were applied. The only farm-level variable associated with high SCC was the method of drying off. Gradual drying off decreased SCC.
Nguyen TTT, Higashi T, Kambayashi Y, EO A, et al. "A Longitudinal study of association between heavy metals and itchy eyes, coughing in chronic cough patients: related with non-immunoglobin E mediated mechanism." International Journal of Environmental Research and Public Health. 2016;13:110.
MBORI- PROFNGACHADOROTHYA. "Longitudinal comparison of chemokines in breastmilk early postpartum among HIV-1-infected and uninfected Kenyan women. Breastfeed Med . 2007 Sep; 2 ( 3 ): 129-38 . PMID: 17903098 [PubMed - indexed for MEDLINE] Bosire R, Guthrie BL, Lohman-Payne B, Mabuka .". In: Breastfeed Med . 2007 Sep; 2 ( 3 ): 129-38 . Earthscan, London. 978-1-84407-469-3 (*); 2007. Abstract
Center for Public Health Research, Kenya Medical Research Institute, Nairobi, Kenya. Breastmilk chemokines have been associated with increased HIV-1 RNA levels in breastmilk and altered risk of mother-to-child HIV-1 transmission. To characterize CC and CXC chemokines in breastmilk postpartum, we collected breastmilk specimens at regular intervals for 6 months after delivery from women with and without HIV-1 infection and used commercial ELISA kits to measure breastmilk concentrations of MIP-1alpha, MIP-1beta, RANTES, and SDF-1alpha. Among 54 HIV-1-infected and 26 uninfected women, mean chemokine levels were compared cross-sectionally and longitudinally at days 5 and 10, and months 1 and 3 postpartum. For both HIV-1-infected and uninfected women, breastmilk chemokine levels were highest at day 5 for MIP-1alpha, MIP-1beta, and SDF-1alpha, and subsequently decreased. RANTES levels remained constant over the follow-up period among HIV-1-uninfected women, and increased moderately among HIV-1-infected women. For MIP-1beta and RANTES, breastmilk levels were significantly higher among HIV-1-infected women compared to uninfected women early postpartum. In addition, HIV-1-infected women transmitting HIV-1 to their infant had consistently higher breastmilk RANTES levels than those who did not transmit, with the greatest difference observed at 1 month (2.68 vs. 2.21 log10 pg/mL, respectively; p = 0.007). In summary, all four chemokines were most elevated within the first month postpartum, a period of high transmission risk via breastmilk. MIP-1beta and RANTES levels in breastmilk were higher among HIV-1-infected women than among uninfected women, and breastmilk RANTES was positively associated with vertical transmission in this study, consistent with results from our earlier cohort. PMID: 17903098 [PubMed - indexed for MEDLINE]
MBORI- PROFNGACHADOROTHYA, ELIZABETH DROBIMBO. "Longitudinal assessment of human immunodeficiency virus type 1 (HIV-1)-specific gamma interferon responses during the first year of life in HIV-1-infected infants. J Virol . 2005 Jul; 79 ( 13 ): 8121-30 . PMID: 15956557 [PubMed - indexed for MEDLINE] Lohm.". In: J Virol . 2005 Jul; 79 ( 13 ): 8121-30 . Kisipan, M.L.; 2005. Abstract
Department of Paediatrics, University of Nairobi, Kenya. bllohman@iconnect.co.ke Human immunodeficiency virus type 1 (HIV-1) infection results in different patterns of viral replication in pediatric compared to adult populations. The role of early HIV-1-specific responses in viral control has not been well defined, because most studies of HIV-1-infected infants have been retrospective or cross-sectional. We evaluated the association between HIV-1-specific gamma interferon (IFN-gamma) release from the cells of infants of 1 to 3 months of age and peak viral loads and mortality in the first year of life among 61 Kenyan HIV-1-infected infants. At 1 month, responses were detected in 7/12 (58%) and 6/21 (29%) of infants infected in utero and peripartum, respectively (P = 0.09), and in approximately 50% of infants thereafter. Peaks of HIV-specific spot-forming units (SFU) increased significantly with age in all infants, from 251/10(6) peripheral blood mononuclear cells (PBMC) at 1 month of age to 501/10(6) PBMC at 12 months of age (P = 0.03), although when limited to infants who survived to 1 year, the increase in peak HIV-specific SFU was no longer significant (P = 0.18). Over the first year of life, infants with IFN-gamma responses at 1 month had peak plasma viral loads, rates of decline of viral load, and mortality risk similar to those of infants who lacked responses at 1 month. The strength and breadth of IFN-gamma responses at 1 month were not significantly associated with viral containment or mortality. These results suggest that, in contrast to HIV-1-infected adults, in whom strong cytotoxic T lymphocyte responses in primary infection are associated with reductions in viremia, HIV-1-infected neonates generate HIV-1-specific CD8+-T-cell responses early in life that are not clearly associated with improved clinical outcomes. PMID: 15956557 [PubMed - indexed for MEDLINE] PMCID: PMC1143755
MBORI- PROFNGACHADOROTHYA, ELIZABETH DROBIMBO. "Longitudinal assessment of human immunodeficiency virus type 1 (HIV-1)-specific gamma interferon responses during the first year of life in HIV-1-infected infants. J Virol . 2005 Jul; 79 ( 13 ): 8121-30 . PMID: 15956557 [PubMed - indexed for MEDLINE] Lohm.". In: J Virol . 2005 Jul; 79 ( 13 ): 8121-30 . Earthscan, London. 978-1-84407-469-3 (*); 2005. Abstract
Department of Paediatrics, University of Nairobi, Kenya. bllohman@iconnect.co.ke Human immunodeficiency virus type 1 (HIV-1) infection results in different patterns of viral replication in pediatric compared to adult populations. The role of early HIV-1-specific responses in viral control has not been well defined, because most studies of HIV-1-infected infants have been retrospective or cross-sectional. We evaluated the association between HIV-1-specific gamma interferon (IFN-gamma) release from the cells of infants of 1 to 3 months of age and peak viral loads and mortality in the first year of life among 61 Kenyan HIV-1-infected infants. At 1 month, responses were detected in 7/12 (58%) and 6/21 (29%) of infants infected in utero and peripartum, respectively (P = 0.09), and in approximately 50% of infants thereafter. Peaks of HIV-specific spot-forming units (SFU) increased significantly with age in all infants, from 251/10(6) peripheral blood mononuclear cells (PBMC) at 1 month of age to 501/10(6) PBMC at 12 months of age (P = 0.03), although when limited to infants who survived to 1 year, the increase in peak HIV-specific SFU was no longer significant (P = 0.18). Over the first year of life, infants with IFN-gamma responses at 1 month had peak plasma viral loads, rates of decline of viral load, and mortality risk similar to those of infants who lacked responses at 1 month. The strength and breadth of IFN-gamma responses at 1 month were not significantly associated with viral containment or mortality. These results suggest that, in contrast to HIV-1-infected adults, in whom strong cytotoxic T lymphocyte responses in primary infection are associated with reductions in viremia, HIV-1-infected neonates generate HIV-1-specific CD8+-T-cell responses early in life that are not clearly associated with improved clinical outcomes. PMID: 15956557 [PubMed - indexed for MEDLINE] PMCID: PMC1143755
MBORI- PROFNGACHADOROTHYA, W. PROFNDUATIRUTH. "Longitudinal analysis of human immunodeficiency virus type 1 RNA in breast milk and of its relationship to infant infection and maternal disease. Rousseau CM, Nduati RW, Richardson BA, Steele MS, John-Stewart GC, Mbori-Ngacha DA, Kreiss JK, Overbaugh J.". In: J Infect Dis. 2003 Mar 1;187(5):741-7. Epub 2003 Feb 18. Journal of School of Continuous and Distance Education ; 2003. Abstract
Transmission of human immunodeficiency virus type 1 (HIV-1) via breast-feeding can occur throughout lactation. Defining both fluctuation in breast-milk virus level over time and how breast-milk virus correlates with mother-to-child transmission is important for establishing effective interventions. We quantified breast-milk HIV-1 RNA levels in serial samples collected from 275 women for up to 2 years after delivery. Higher maternal plasma virus load, lower maternal CD4 T cell count, and detection of HIV-1 DNA in maternal genital secretions were significantly associated with elevated breast-milk HIV-1 RNA. Within women who breast-fed, median virus load in colostrum/early milk was significantly higher than that in mature breast milk collected 14 days after delivery (P< or =.004). Breast-feeding mothers who transmitted HIV-1 to their infants had both significantly higher breast-milk viral RNA throughout lactation and more-consistent viral shedding, compared with mothers who did not transmit HIV-1. In breast-feeding women, a 2-fold-increased risk of transmission was associated with every 10-fold increase in breast-milk virus load (95% confidence interval, 1.3-3.0; P<.001). These results indicate that the risk of infant infection from breast-feeding is influenced by breast-milk virus load, which is highest early after delivery.
MBORI- PROFNGACHADOROTHYA, W. PROFNDUATIRUTH. "Longitudinal analysis of human immunodeficiency virus type 1 RNA in breast milk and of its relationship to infant infection and maternal disease. Rousseau CM, Nduati RW, Richardson BA, Steele MS, John-Stewart GC, Mbori-Ngacha DA, Kreiss JK, Overbaugh J.". In: J Infect Dis. 2003 Mar 1;187(5):741-7. Epub 2003 Feb 18. Earthscan, London. 978-1-84407-469-3 (*); 2003. Abstract
Transmission of human immunodeficiency virus type 1 (HIV-1) via breast-feeding can occur throughout lactation. Defining both fluctuation in breast-milk virus level over time and how breast-milk virus correlates with mother-to-child transmission is important for establishing effective interventions. We quantified breast-milk HIV-1 RNA levels in serial samples collected from 275 women for up to 2 years after delivery. Higher maternal plasma virus load, lower maternal CD4 T cell count, and detection of HIV-1 DNA in maternal genital secretions were significantly associated with elevated breast-milk HIV-1 RNA. Within women who breast-fed, median virus load in colostrum/early milk was significantly higher than that in mature breast milk collected 14 days after delivery (P< or =.004). Breast-feeding mothers who transmitted HIV-1 to their infants had both significantly higher breast-milk viral RNA throughout lactation and more-consistent viral shedding, compared with mothers who did not transmit HIV-1. In breast-feeding women, a 2-fold-increased risk of transmission was associated with every 10-fold increase in breast-milk virus load (95% confidence interval, 1.3-3.0; P<.001). These results indicate that the risk of infant infection from breast-feeding is influenced by breast-milk virus load, which is highest early after delivery.
MBURU STEPHEN. "Longhorn Secondary Computer Studies Series (Form 1 to Form 4 books.". In: European Journal of Pharmaceutical sciences. Longhorn; 2004.
MBURU STEPHENNGANGA. "Longhorn ICT for Teacher Training Colleges (manuscript).". In: European Journal of Pharmaceutical sciences. Longhorn; 2010.
MBURU MRSTEPHENNGANGA. "Longhorn Gateway Computer Studies Series Revision Guide.". In: European Journal of Pharmaceutical sciences. Longhorn; 2008.
Wamalwa DC, Lehman DA, Benki-Nugent S, Gasper MA, Gichohi R, Maleche-Obimbo E, Farquhar C, John-Stewart GC, Overbaugh J. "Long-term virologic response and genotypic resistance mutations in HIV-1 infected Kenyan children on combination antiretroviral therapy." J. Acquir. Immune Defic. Syndr.. 2013;62(3):267-74. Abstract

HIV-infected children may require the use of combination antiretroviral treatment (cART) into adulthood. However, regimens are limited to first line and second line in many African settings. Therefore, understanding the long-term rate of virologic failure and drug resistance during prolonged antiretroviral treatment is important for establishing treatment strategies in African pediatric cohorts.

Odada EO, Olago DO, Street-Perrott FA, Perrott RA, Ivanovich M, M.; Harkness DD. "Long-term temporal characteristics of palaeomonsoon dynamics in equatorial Africa." M.; Harkness, D.D. 2000; vol. 26( issue 1-3):159-171. AbstractWebsite

In this paper we examine the long-term temporal characteristics of palaeomonsoon dynamics in equatorial Africa from a continuous lacustrine sequence retrieved from Sacred Lake, Mount Kenya (0°03′N, 37°32′E, 2350 m a.s.l.), covering the last interglacial–glacial transition to the present. The trends in mineral magnetics and stable carbon isotopes are proxy indicators of changes in precipitation on the mountain over the last glacial–interglacial cycle. Spectral analysis by a fast fourier transform method revealed that the stable carbon isotope trend (δ13C) has strong signals at the 23,000 and 11,500 year frequencies. The mineral magnetic signature does not register the 23,000 year cycle observed in the δ13C signature. It has, however, a strong signal at an 11,500 year frequency, and sharp but relatively weak peaks at ca. 7500 and 5000 year frequencies are recorded. The dominant 23,000 year frequency recorded in the δ13C signature reflects the strong effect of the precessional cycle on tropical climate and ecosystems, and is most probably effected via global atmospheric pCO2 and temperature changes. The shorter cycles at 11,500 year (indicated by both mineral magnetics and δ13C trends), and 7500 and 5000 years BP (apparent in the mineral magnetic record) are attributed to precipitation variations, whose temporal cycles are dominated by the higher precessional harmonics.

Keywords: paleomoonsoon; mineral magnetics; stable carbon isotopes; Milankovitch forcing

JOAB PROFBWAYOJOB, O. PROFANZALAAGGREY. "Long-term survivors in Nairobi: complete HIV-1 RNA sequences and immunogenetic associations. Fang G, Kuiken C, Weiser B, Rowland-Jones S, Plummer F, Chen CH, Kaul R, Anzala AO, Bwayo JJ, Kimani J, Philpott SM, Kitchen C, Sinsheimer JS, Gaschen B, Lang D, .". In: Burger HJ Infect Dis. 2004 Aug 15;190(4):697-701. Asian Economic and Social Society; 2004. Abstract
Background. The host immune response against mucosally-acquired pathogens may be influenced by the mucosal immune milieu during acquisition. Since Neisseria gonorrhoeae can impair dendritic cell and T cell immune function, we hypothesized that co-infection during HIV acquisition would impair subsequent systemic T-cell responses.   Methods. Monthly screening for sexually transmitted infections (STIs) was performed in high risk, HIV seronegative Kenyan female sex workers as part of an HIV prevention trial. Early HIV-specific CD8+ T cell responses and subsequent HIV viral load set point were assayed in participants acquiring HIV, and were correlated with the presence of prior genital infections during HIV acquisition.   Results. Thirty-five participants acquired HIV during follow up, and 16/35 (46%) had a classical STI at the time of acquisition. N. gonorrhoeae co-infection was present during HIV acquisition in 6/35 (17%), and was associated with an increased breadth and magnitude of systemic HIV-specific CD8+ T-cell responses, using both interferon- (IFNg) and MIP-1 beta (MIP1b) as an output. No other genital infections were associated with differences in HIV-specific CD8+ T cell response, and neither N. gonorrhoeae nor other genital infections were associated with differences in HIV plasma viral load at set point.   Conclusion. Unexpectedly, genital N. gonorrhoeae infection during heterosexual HIV acquisition was associated with substantially enhanced HIV-specific CD8+ T-cell responses, although not with differences in HIV viral load set point. This may have implications for the development of mucosal HIV vaccines and adjuvants.
JOAB PROFBWAYOJOB, O. PROFANZALAAGGREY. "Long-term survivors in Nairobi: complete HIV-1 RNA sequences and immunogenetic associations. Fang G, Kuiken C, Weiser B, Rowland-Jones S, Plummer F, Chen CH, Kaul R, Anzala AO, Bwayo JJ, Kimani J, Philpott SM, Kitchen C, Sinsheimer JS, Gaschen B, Lang D, .". In: Burger HJ Infect Dis. 2004 Aug 15;190(4):697-701. John Benjamins Publishing Company; 2004. Abstract
OBJECTIVE: To determine the prevalence of HCV infection and HCV/HIV co-infection among voluntary blood donors at the National Blood Transfusion Centre and clients at the Kenyatta National Hospital HIV-Voluntary Counseling and Testing (VCT) Centre. DESIGN: A prospective cross-sectional descriptive study. SETTING: Kenyatta National Hospital, a tertiary referral and teaching hospital and the National Blood Transfusion Services Centre, Nairobi. SUBJECTS: Volunteer blood donors and VCT attendants. RESULTS: The prevalence of HCV/HIV co-infection among 6154 blood donors in the NBTSC was very low, at 0.02. The HIV prevalence among the 353 KNH HIV-VCT clients was 9.3%, none of the clients tested positive for HCV. The incidence of risk factors in the persons with HCV and/or HIV infection(s) was low. CONCLUSION: The prevalence of HCV infection among pre-screened volunteer blood donors was low. However the current practice of screening all donated blood for HCV remains indispensable to prevent its transmission to blood recipients.
1. Fang G, Kuiken C WR-JPCCHKAAOBKPSMKBSF. "Long-term survivors in Nairobi: complete HIV-1 RNA sequences and immunogenetic associations." J Infect Dis. 2004. Abstractlong_term_survivors_in_nairobi_complete_hiv_1_rna_sequences_and_immunogenetic_associations.pdf

Abstract To investigate African long-termsurvivors (LTSs) infected with non-subtype B human immunodeficiency virus type 1 (HIV- 1), we obtained full-length HIV-1 RNA sequences and immunogenetic profiles from 6 untreated women enrolled in the Pumwani Sex Worker Cohort in Nairobi, Kenya. There were no discernible sequence changes likely to cause attenuation. CCR2-V64I, an immunogenetic polymorphism linked to LTSs, was detected in 4 women, all of whom carried the HLA B58 allele. Further investigation of 99 HIV-1-infected Nairobi women found an association between CCR2-V64I and HLA B58 (P = .0048). Studying the interaction among immunogenetics, immune responses, and viral sequences from all HIV- 1 subtypes may increase our understanding of slow HIV-1 disease progression. Sub-Saharan Africa accounts for 70% of HIV-1-infected individuals globally, and infected women in this region outnumber men. The study of long-term survivors (LTSs) is relevant to pathogenesis and the design of an HIV-1 vaccine. The HIV- 1 subtypes and host immunogenetics of LTSs in Africa differ from those of most LTSs studied previously [6–6]; for example, the HLA types are more diverse [6], and Δ32 mutations in coreceptor CCR5 are rarely seen [4]. Recombination between different HIV-1 subtypes has been well documented [2, 3, 7] and, along with viral diversity, is also relevant to the design of a vaccine. Analysis of both viral diversity and intersubtype recombination would benefit from the sequencing of entire viral genomes derived from plasma virions. The examination of plasma HIV-1 RNA offers an opportunity to observe the replicating virus population, including recombinant genomes in circulating viral particles. Nairobi sex workers, who are exposed to a range of viral strains, may be infected with intersubtype recombinants. To investigate pathogenesis in women with non-clade B HIV-1 infection, we analyzed complete HIV-1 RNA sequences, immunogenetic traits, immune responses, coreceptor utilization, and drug resistance in untreated LTSs from Kenya. Subjects and methods. The subjects were untreated HIV- 1-infected adult women enrolled in the Pumwani Sex Worker Cohort in Nairobi, Kenya [1]. The research was approved by the Kenyatta National Hospital National Ethical and Scientific Review Committee, the University of Manitoba Use of Human Subjects in Research Committee, and the New York State Department of Health Institutional Review Board. Within this cohort, long-term nonprogressors (LTNPs) and LTSs were identified [1]. Both LTSs and LTNPs had been infected with HIV-1 for ⩾10 years, and LTNPs had maintained CD4+ T cell counts ⩾500 cells/μL. To detect coreceptor polymorphisms, human genotyping was performed as
described elsewhere [4, 8]. Extraction of viral RNA from plasma, reverse transcription, long polymerase chain reaction (PCR) amplification, and analysis of full-length HIV-1 sequences were performed as described elsewhere [7]. Phylogenetic trees were constructed, and HIV-1 subtypes and recombinants were determined as described elsewhere [7]. Full-length HIV-1 env genes were cloned from plasma, and coreceptor usage was determined phenotypically by the use of GHOST cells [9]. The V3 loop sequence of env clones was also determined and was used to genotypically predict coreceptor utilization [10]. Molecular class I HLA types were determined as described elsewhere [6]. Neutralizing antibodies were detected as described elsewhere [11]. Genotypic resistance to antiretroviral agents was analyzed by the ADRA program [2]; phenotypic resistance was measured by the PhenoSense assay [12]. Several nonparametric tests of association were used to correlate the immunogenetic data with the virologic and clinical data. A 2-sided Wilcoxon rank sum test was used to test differences between continuous measures such as the number of CD4+ T cells and viral load. Fisher's exact test was used to analyze HLA types and coreceptor polymorphisms. A number of genetic analyses were performed by use of the Mendel statistical package [13]. Hardy-Weinberg-equilibrium (HWE) testing was used to examine whether the genotype frequencies for single loci were equal to products of the population allele frequencies. Gamete-phase equilibrium testing, a generalization of linkage-equilibrium testing that allows one to consider loci on different chromosomes, would normally be used to examine whether the joint frequencies of different alleles at several loci derived from the same parent are the product of the underlying population allele frequencies. To test gamete-phase equilibrium (or linkage equilibrium), however, knowledge of the parental source of the alleles at a locus (i.e., phase information) is required. Because phase information was unavailable but multilocus genotypes were known, we instead tested for genetic equilibrium. Genetic equilibrium holds only when both HWE and gamete-phase equilibrium are maintained. If genetic equilibrium was violated but separate tests of HWE were not rejected at all loci, then we assumed that genetic disequilibrium was a result of gamete-phase disequilibrium. Results. Table 1 shows the clinical, virologic, immunologic, and immunogenetic characteristics of 6 subjects in the Nairobi female sex-worker cohort who had been infected with nonsubtype B HIV-1 for ⩾10 years. Virions were isolated from plasma obtained from all 6 subjects in 1997 and also from additional plasma obtained in 1986 from subject ML013. The complete RNA genome was reverse-transcribed, amplified by long PCR, and directly sequenced. View larger version: In this page
In a new window Download as PowerPoint Slide Table 1. Virologic and immunogenetic characteristics in 6 untreated women in the Pumwani Sex Worker Cohort in Nairobi, Kenya. Complete HIV-1 RNA sequences were assembled, aligned, and analyzed by computational methods [7]. GenBank accession numbers are shown in table 1. Three subjects—ML752, ML013, and ML605—were infected with HIV-1 genomes identified as entirely subtype A; both the 1986 and the 1997 samples from ML013 also displayed subtype A genomes. Subject ML415 was infected with a viral genome identified as entirely subtype D. Two subjects displayed HIV-1 genomes that were intersubtype recombinants. Virus from ML672 was composed predominantly of clade A sequences with a clade C fragment in the pol gene. Subject ML249's recombinant virus was predominantly composed of clade D but also displayed a clade C fragment in nef and the 3' long terminal repeat. Sequences were examined for mutations that might contribute to attenuation of HIV-1. It is possible that single-nucleotide changes might help to attenuate the virus, and it was reported recently that R77Q, a mutation in the HIV-1 vpr gene, is associated with both LTNP infection and impaired induction of apoptosis [14]. This mutation was present in 3 of the 6 women studied, including 2 of the LTNPs (table 1); the association, however, was not statistically significant. No other clearly attenuating mutations or deletions were detected, nor any polymorphisms common to more than 1 sequence. We determined human genotypes for HIV-1 coreceptors, coreceptor-associated genes, and HLA class I haplotypes, to examine the contribution of immunogenetics to LTSs (table 1). All 6 subjects had homozygous wild-type CCR5 genotypes. Four subjects exhibited polymorphisms in the CCR2 gene; 2 LTNPs (ML672 and ML752) were homozygous for the V64I mutation, and 2 LTS subjects (ML013 and ML605) were heterozygous for it. It is noteworthy that all 4 women who carried the V64I allele also displayed the B58 HLA haplotype. Statistical analyses showed an association between the presence of the CCR2-V64I mutation (in at least 1 allele) and HLA type B58 (P=.06). To explore this association further, we expanded our immunogenetic analysis to include a larger group of 167 women in the Nairobi sex-worker cohort [1]. In addition to CCR2 and HLA B58, we examined the SDF-1α-3' untranslated region, bringing the total to 3 human genes, each located on a different chromosome [4]. There was no significant association between the CCR2 mutation and the SDF mutation. As shown in table 2, 99 (59.3%) of the 167 women were HIV-1 seropositive and 68 (40.7%) were HIV-1 seronegative. The B58 allele was of interest; all other alleles were combined, and the locus was treated as biallelic. No significant deviations from HWE were found for either CCR2 or HLA-B, either in the entire sample of 167 women or in the groups
stratified by HIV serostatus. In contrast, we did find, in the entire sample, significant evidence for gamete-phase disequilibrium between CCR2 and HLA B (P=.00780), indicating a highly significant association between CCR2-V64I and HLA type B58. This association was also significant in the HIV-seropositive subjects (P=.00486), but not in the HIV-seronegative subjects. View larger version: In this page In a new window Download as PowerPoint Slide Table 2. CCR2 mutations and HLA B58 in 167 Kenyan women in the Pumwani Sex Worker Cohort in Nairobi, Kenya. We determined CCR5-promoter genotypes (table 1). One LTNP (ML415) and one LTS (ML249) were homozygous for CCR5-59029G, a polymorphism associated with delayed progression of HIV-1 disease [4]. Coreceptor usage was determined for HIV-1 envelope clones obtained from 5 subjects (table 1). The majority (71/77 [92.2%]) of clones utilized CCR5. A minority of CXCR4-utilizing species were also detected in 3 subjects (ML672, ML752, and ML605). No significant drug-resistance mutations were seen. The Pheno- Sense assay was used to examine phenotypic resistance; only a specimen from subject ML415 gave a result, and no resistance was found. Although the absence of viable cells precluded functional studies of CTL activity, we were able to predict, on the basis of the donor HLA haplotype and predicted epitopes found in the immunology databases in the Los Alamos National Laboratory and Oxford University, the likely sites of CTL recognition. At least half the predicted epitopes carried 1 or more amino acid changes from the consensus sequence (data not shown); however, KAFSPEVIPMF, the immunodominant target of CTL recognition through HLA-B57 and B58 in HIV-1 gag, was conserved in all donors [15]. Neutralizing antibody titers ranged from negative to 1:640 (table 1). The serum demonstrated neutralization against strain MN (clade B) but not against strain 92/UG/31 (clade A). Discussion. This study is one of the first to characterize female LTSs and LTNPs from Africa, where both HIV-1 subtypes and immunogenetic traits differ from those of LTSs studied previously. One of the remarkable features of these LTSs is their fairly high viral loads (table 1).
These Kenyan subjects managed to survive, most of them as asymptomatics, for periods of 12–16 years, without antiretroviral treatment. Computational analyses of the complete HIV-1 RNA sequences confirmed both the frequency of intersubtype recombination and the particular HIV-1 subtypes observed in a recent study from Kenya [3]. One LTNP (ML672) and 1 LTS (ML249) had recombinant genomes. The sequence data, which are derived from plasma virions, provide direct evidence of recombinant genomes in circulating viral particles. Computational analyses of the sequences did not reveal any clearly attenuating mutations except for the vpr R77Q mutation (table 1) [14]; in our small study, the association between this mutation and LTSs was suggestive but not significant. All of the sequences analyzed in this study appeared to be intact and gave no indication that they coded for nonfunctional proteins. In fact, when multiple viral env genes from these subjects were cloned into an expression system to determine coreceptor utilization, most clones yielded functional envelopes. Although it is possible that 1 or more point mutations in the viral genomes may have diminished the pathogenicity of the viruses infecting these 6 women, we did not identify any deletions or mutations that would clearly confer attenuation on any of the viruses. Immunogenetics may have contributed to LTS status in this study (table 1). CCR2-V64I, previously linked to LTSs, was detected in 4 women. A highly significant correlation between the presence of the CCR2-V64I mutation and the HLA B58 allele was found in 167 women in the Nairobi cohort. The association was particularly strong in the 99 women who were HIV-1 seropositive, although it was not significant in the 68 women who were HIV-1 seronegative. The stronger association between these 2 alleles in the infected women, compared with that in the uninfected women, may reflect, in this group, a selection for LTSs bearing both V64I and B58 genes. An alternative explanation, however, is possible, reflecting the genetics of the population under study: when 2 loci are close to each other on a chromosome, departure from gamete-phase equilibrium is often taken as evidence for linkage disequilibrium; because CCR2 and HLA B genes are on different chromosomes, departure fromgenetic equilibrium is likely due to recent ethnic admixture in these subjects; however, joint selective pressures may also be acting on the 2 genes. The association of these human genes, CCR2 and HLA B58, has not been previously noted, and it may possibly provide a clue to the manner in which CCR2 affects the pace of HIV-1 infection. Although multiple studies, including 1 focusing on the Nairobi cohort [5], have reported that the CCR2-V64I allele may slow the progression of HIV-1 disease, the mechanism by which the mutation acts is still unclear [4, 5]. The HLA B57 allele, which is related to B58, has also been associated with both slowed progression of disease and long-term survival [6, 15]. The close association, in HIV-1-infected women, between the CCR2 mutation and B58 suggests that the V64I allele may affect the pace of HIV-1 infection in part or entirely through the HLA B58 haplotype. This question necessitates further investigation. Finally, these studies suggest that
studying the interaction among immunogenetics, immune responses, and viral sequences from all HIV-1 subtypes may increase our understanding of the slow progression of HIV-1 disease.

O DROGARAWILLIAM. "Long-term performance of electronic identification devices and model traceability system for cattle under pastoral production systems of Kenya.". In: Journal. Livestock Research for Rural Development; Submitted. Abstract
G O Matete*, W Maritim**, G Muchemi**, N Maingi***, J M Gathuma* and W Ogara* * Department of Veterinary Public Health, Pharmacology and Toxicology, University of Nairobi P.O Box 30197, Nairobi, Kenyageorge.matete@gmail.com** Ministry of Livestock Development P.O Kabete 00625 Kangemi, Nairobi, Kenya*** Department of Veterinary Pathology and Parasitology, University of Nairobi, P.O Box 30197, Nairobi, Kenya Abstract The readability of two different types of electronic identifiers (EID) were evaluated under pastoral production system in North-Eastern Kenya.  Physical verification and reading was done at day 0, and 1, 2, 4, 8 and 12 months respectively on a total of 1943 beef cattle of which 934 were tagged using ear button tags and 1009 with rumen boluses.  The retention rates were recorded and readability determined using a hand-held reader and subsequently compared using a non parametric survival analysis.   The results showed that, rumen boluses were more effective with retention and readability of 100% after the one-year period.  The retention rate for ear button tags deteriorated after day 120 to 94.6%.  This implied that rumen boluses are safe and tamper-proof and are thus recommended for use in pastoral production systems. When tested within the model Livestock Identification and Traceability System (LITS), the use of RFID identifiers were able to substantially contribute to better record keeping, and proof of credible livestock certification. However, due to cost considerations, undertaking a benefit-cost analysis and provisional analysis of the institutional and organisational infrastructure may be critical for successful implementation. Keywords: livestock identification, radio frequency identification devices, traceability system
O DROGARAWILLIAM. "Long-term performance of electronic identification devices and model traceability system for cattle under pastoral production systems of Kenya.". In: Journal. Livestock Research for Rural Development; Submitted. Abstract
G O Matete*, W Maritim**, G Muchemi**, N Maingi***, J M Gathuma* and W Ogara* * Department of Veterinary Public Health, Pharmacology and Toxicology, University of Nairobi P.O Box 30197, Nairobi, Kenyageorge.matete@gmail.com** Ministry of Livestock Development P.O Kabete 00625 Kangemi, Nairobi, Kenya*** Department of Veterinary Pathology and Parasitology, University of Nairobi, P.O Box 30197, Nairobi, Kenya Abstract The readability of two different types of electronic identifiers (EID) were evaluated under pastoral production system in North-Eastern Kenya.  Physical verification and reading was done at day 0, and 1, 2, 4, 8 and 12 months respectively on a total of 1943 beef cattle of which 934 were tagged using ear button tags and 1009 with rumen boluses.  The retention rates were recorded and readability determined using a hand-held reader and subsequently compared using a non parametric survival analysis.   The results showed that, rumen boluses were more effective with retention and readability of 100% after the one-year period.  The retention rate for ear button tags deteriorated after day 120 to 94.6%.  This implied that rumen boluses are safe and tamper-proof and are thus recommended for use in pastoral production systems. When tested within the model Livestock Identification and Traceability System (LITS), the use of RFID identifiers were able to substantially contribute to better record keeping, and proof of credible livestock certification. However, due to cost considerations, undertaking a benefit-cost analysis and provisional analysis of the institutional and organisational infrastructure may be critical for successful implementation. Keywords: livestock identification, radio frequency identification devices, traceability system
Riha SJ;, Blume LE;, Barret CB;, Kinyangi JM;, Lehmann CJ;, Marenya PP;, Mbugua DM;, Nicholson CF;, Ngoze SO;, Parsons D;, Verchot LV;, Pell AN. "Long-Term Human and Biophysical Dynamics of Soil Degradation in the Kenyan Highlands.".; 2006. Abstract

Agroecosystems are among the most tightly coupled of human and natural systems, as farmers make conscious decisions regarding land use and improvement, cropping systems, livestock management and labor allocation. These decisions can profoundly impact the natural resource base, which can then lead to changes in farmers' behaviors. The focus of this study is to understand the long term human and biophysical dynamics of soil degradation. We are especially interested in the role that soil degradation plays in creating poverty traps and in interventions that will strongly impact the dynamics of these systems. We have developed an integrated economic and biophysical systems dynamic model to understand and predict the long term behavior of farms in the Kenyan highlands. Additionally, we have established a chronosequence in western Kenya of farms converted from primary forest to agriculture 100, 70, 50, 30, 15, 5, and < 3 years ago. This chronosequence includes three blocks that contain all time conversions, with 3 farms per conversion. Soil chemistry and soil organic matter fractions have been measured from fields that have never received fertilizer additions. An extensive set of fertility experiments to examine the response of maize to amendment with nitrogen and phosphorus fertilizers, manure and green manure have been established on these soils. Socioeconomic data for these farms has been collected. The chronosequence data is being used to both parameterize and evaluate the model. Preliminary findings indicate that both soil organic matter and maize yields decline after conversion from primary forest, but not at the same rates. As the soil degrades and maize yields decrease, farms become more diversified by shifting some land into perennials. This change in land use is associated with a stable, though decreased, soil fertility level. The relationship of these changes in cropping systems and soil fertility to off farm activities and income will be discussed, as well as the implications of these dynamics for preventing soil degradation and restoring fertility.

Schmidt C, Jaoko W O-MKMNKBBLGGPJA, Chomba E, Kilembe W NNSCLCADSFPEMJGP. "Long-term follow-up of study participants from prophylactic HIV vaccine clinical trials in Africa." Human Vaccines & Immunotherapy. 2014;10(3):714-23.
Schmidt, C. JO-MKMNKBWGPJ, L. G. Bekker, E. Chomba KNNSCLWMJG, and J. Cox, S. Allen DSFLCPE. "Long-Term Follow-up of Study Participants from Prophylactic Hiv Vaccine Clinical Trials in Africa." Hum Vaccin Immunother. 2014;10, no. 3:714-23.
Ackello-Ogutu, Chris; Okoruwa V; BGN. Long-term challenges to food security and rural livelihoods in Sub-Saharan Africa.; 2012. Abstract

This briefing paper is one of the 10-part Global Development Network (GDN) Agriculture Policy Series for its project, ‘Supporting Policy Research to Inform Agricultural Policy in Sub-Saharan Africa and South Asia’. It is based on a longer synthesis paper, Long-term challenges to food security and rural livelihoods in Sub-Saharan Africa, which draws on extensive published and unpublished research. It will be of value to policymakers, experts and civil society working to improve agriculture in Sub-Saharan Africa. A total of 180 million food-insecure people live in Sub-Saharan Africa, over 21 per cent of the African population. These are among the world’s most vulnerable people, poorly equipped to respond to the threat of climate change, demographic stresses, or spikes in global food prices. This briefing examines how improvements in agriculture might help to achieve g

ODUOR PROFOGENDOSTEPHEN. "Long term valve-related morbidity following open-heart surgery at the Kenyatta National Hospital.". In: East Afr Med J. 2000 Apr;77(4):199-202. The East and Central African Journal of Surgery.; 2000. Abstract
OBJECTIVE: To determine the incidence of valve morbidity associated with post-operative valve patients at the Kenyatta National Hospital, Nairobi. DESIGN: A combined retrospective and prospective study. Restrospective period from 1973 to 31st July 1977 and prospective period from there on to 31st July 1998. SETTING: Cardiothoracic outpatient clinic at Kenyatta National Hospital, Nairobi. PATIENTS: All valve replacement patients attending the cardiothoracic outpatient clinic on follow up following discharge from the ward. OUTCOME MEASURES: Morbidity was assessed by incidence of: stroke, thromboembolism, valve thrombosis, bleeding episodes, valve endocarditis and re-operation for valve failure. RESULTS: For all valve surgery combined the overall follow up rate equalled 476.3 patient years. The linearised occurrence rate for thromboembolism for all valves was 1.04% per patient year. For the mechanical valves this incidence was 1.32% per patient year. The stroke free rate at one year and five years was 97.7% and 95.9% respectively. The linearised incidence rate for stroke was 0.84% per patient year for all valves. The incidence of bleeding episodes, excluding menorrhagia, for mechanical valves was 0.79% per patient year. Three episodes of valve thrombosis were observed in this study group, all occurring with mechanical valves. For mechanical valves the incidence equalled 0.79% per patient year. The incidence of prosthetic valve endocarditis equalled 0.42% per patient year for all valves combined, or 0.53% per patient year for mechanical valves. Three patients in the series had redo surgery due to valve failure (0.63%). The commutative occurrence of valve related morbidity for the study population for all valves analysed was 2.73% per patient year. CONCLUSION: The incidence of the various valve morbidity at the Kenyatta National Hospital are below the internationally quoted figures in the literature for other centres. The high incidence of drop out to clinic follow up is likely to be the main reason for this with a lot of morbidity occurring outside the hospital environment and therefore not documented. The exact estimation of this incidence is difficult to assess. To help ascertain this occurrence an accurate patient database with accurate (real time) contacts needs to be put into place.
K DRMUSAMBAYICHRISANTHUSIKALIKHA. "Long term policy perspecives for Kenya:Joint IPAR/AERC Seminar report.". In: Joint IPAR / AERC Seminar Report 1999 with P. Kimuyu. uon press; 1999. Abstract
{ The Blantyre coma scale (BCS) is used to assess children with severe falciparum malaria, particularly as a criterion for cerebral malaria, but it has not been formally validated. We compared the BCS to the Adelaide coma scale (ACS), for Kenyan children with severe malaria. We examined the inter-observer agreement between 3 observers in the assessment of coma scales on 17 children by measuring the proportion of agreement (PA), disagreement rate (DR) and fixed sample size kappa (kappa n). We assessed the sensitivity and specificity of the scales in detecting events (seizures and hypoglycaemia) in 240 children during admission and the usefulness of the scales in predicting outcome. There was considerable disagreement between observers in the assessment of both scales (BCS: PA = 0.55
and Muthama AKH, W MJ, N MUTHAMAJ. "Long Term Change Point Detections in Total Ozone Column over East Africa via Maximal Overlap Discrete Wavelet Transform." American Research Journal of Physics. 2016;2(2):1-9.
"The Long Road to a PhD: Choices Changes or Chances." Commonwealth Scholarship News. 2007;1(1):9.
JOAB PROFBWAYOJOB. "Long distance truck-drivers: 1. Prevalence of sexually transmitted diseases (STDs). Bwayo JJ; Omari AM; Mutere AN; Jaoko W; Sekkade-Kigondu C; Kreiss J; Plummer FA. East Afr Med J. 1991 Jun;68(6):425-9.". In: East Afr Med J. 1991 Jun;68(6):425-9. Asian Economic and Social Society; 1991. Abstract
The factors responsible for the explosive spread of human immunodeficiency virus type 1 (HIV-1) in sub-Saharan Africa continue to be identified and debated. One of the most controversial factors has been male circumcision. This cross-sectional study was conducted to measure the association between circumcision status and infection with HIV-1 among men with genital ulcer disease. Eight hundred and ten men participated in the study, of whom 190 (23%) were HIV-1-positive. A logistic regression model adjusted for behavioral and historical showed that HIV-1 positivity was independently associated with being uncircumcised (adjusted odds ratio [OR], 4.8; 95% confidence interval [CI], 3.3-7.2) and with a history of urethral discharge (adjusted OR, 2.0; 95% CI, 1.4-2.8). This association could not be explained by measures of sexual exposure to HIV-1 among this population. Male circumcision should be considered as an intervention strategy for AIDS control.
JOAB PROFBWAYOJOB. "Long distance truck drivers. 2:Knowledge and attitudes concerning sexually transmitted diseases and sexual behaviour. Bwayo JJ; Mutere AN; Omari MA; Kreiss JK; Jaoko W; Sekkade-Kigondu C; Plummer FA. East Afr Med J. 1991 Sep;68(9):714-9.". In: East Afr Med J. 1991 Sep;68(9):714-9. Asian Economic and Social Society; 1991. Abstract
The factors responsible for the explosive spread of human immunodeficiency virus type 1 (HIV-1) in sub-Saharan Africa continue to be identified and debated. One of the most controversial factors has been male circumcision. This cross-sectional study was conducted to measure the association between circumcision status and infection with HIV-1 among men with genital ulcer disease. Eight hundred and ten men participated in the study, of whom 190 (23%) were HIV-1-positive. A logistic regression model adjusted for behavioral and historical showed that HIV-1 positivity was independently associated with being uncircumcised (adjusted odds ratio [OR], 4.8; 95% confidence interval [CI], 3.3-7.2) and with a history of urethral discharge (adjusted OR, 2.0; 95% CI, 1.4-2.8). This association could not be explained by measures of sexual exposure to HIV-1 among this population. Male circumcision should be considered as an intervention strategy for AIDS control.
HENRY PROFINDANGASI. ""Lonely and Diminished Men", A Review of Voices in the Dark by Leonard Kibera in Busara III, 1, 56-59.". In: (Published in Japanese). GIGA German Institute of Global and Area Studies, Hamburg, July 2009; 1970. Abstract
This integrative review on the teaching of reading in Kenyan primary schools provides a foundation for the growing movement there to improve reading education. In gathering sources for this review, we took an inclusive historical stance. Thus, we did not dismiss research reports that lacked traditional indicators of quality such as being published in peer-reviewed journals. We used multiple methods to find relevant research and associated documents, including two trips to Kenya. The review is organized by six topics: (a) language of instruction, (b) reading instruction, (c) reading materials, (d) reading culture, (e) assessment, and (f) teacher development. The review concludes with six proposals for policymakers, educational researchers, and teacher educators for the development of reading instruction based on what we learned in reviewing the literature. The first proposals are intended specifically to address the teaching of reading in Kenya, but they may be relevant to other sub-Saharan nations. The final proposal encourages others to conduct similar reviews to make possible a handbook of reading in Africa.
W. PROFMAKAWITIDOMINIC. "Lomo PO, Makawiti DW, Konji VN.Thyroid status and adenosine triphosphatase activity in experimental Trypanosoma congolense infection in rabbits. Br Vet J. 1996 Nov;152(6):659-67.". In: Br Vet J. 1996 Nov;152(6):659-67. Groebner-Bases-Bibliography-RICAM; 1996. Abstract
The effect of trypanosome infection on the plasma levels and ratios of tri-iodothyronine (T3) and thyroxine (T4) as well as the activity of mitochondrial adenosine triphosphatase (ATPase) were investigated. Three groups of sexually mature white New Zealand rabbits were used. Group 1 consisted of the normal non-infected rabbits, group 2 were experimentally infected with Trypanosoma congolense and group 3 were infected but given replacement doses of thyroxine. The infected animals (group 2) showed a rapid decline in both T3 and T4 but an increase in the T3/T4 ratio indicating differential production or clearance rates between the two hormones. The mitochondrial ATPase activity was found to be depressed in the infected group whereas there was no significant difference in the ATPase activity between the non-infected (group 1) and infected-treated animals (group 2). It is postulated that trypanosome induced hypothyroid status may play a role in the impairment of mitochondrial ATPase activity, a key enzyme in energy metabolism.
W. PROFMAKAWITIDOMINIC. "Lomo PO, Makawiti DW, Konji VN.Respiratory activity of isolated liver Mitochondria following Trypanosoma congolense infection in rabbits: the role of thyroxine. Comp Biochem Physiol B. 1993 Jan;104(1):187-91.". In: Comp Biochem Physiol B. 1993 Jan;104(1):187-91. Groebner-Bases-Bibliography-RICAM; 1993. Abstract
The effect of trypanosome infection on rabbit liver mitochondrial oxidative phosphorylation was investigated, with and without thyroxine replacement. 2. State 3 respiration, respiratory control ratio (RCR) and ADP/O ratio were significantly reduced in mitochondria from trypanosome-infected animals whereas there was no change in state 4 respiration. 3. State 3 respiration, RCR and ADP/O ratio were not significantly altered in trypanosome-infected animals given thyroxine replacement therapy. 4. Trypanosome infection leads to impairment of mitochondrial integrity, apparently through lowered thyroxine levels. Replacement of thyroxine therefore sustains optimal mitochondrial respiratory activity.
W. PROFMAKAWITIDOMINIC. "Lomo PO, Makawiti DW, Konji VN. The effect of L-thyroxine on the anaemia response in Trypanosoma congolense infected rabbits. Vet Parasitol. 1995 Jun;58(3):227-34.". In: Vet Parasitol. 1995 Jun;58(3):227-34. Groebner-Bases-Bibliography-RICAM; 1995. Abstract

{ The development of anaemia is a major pathological manifestation in chronic trypanosomosis. The anaemia in African trypanosomosis coincides with a marked decrease in plasma concentration of both thyroxine (T4) and 3,5,3' triiodothyronine (T3). To evaluate the effect of trypanosome-induced hypothyroidism on the development of anaemia, sexually mature white New Zealand rabbits were used. Three groups were set up, each of ten rabbits: one group was infected with Trypanosoma congolense; the second group was infected but given replacement doses of thyroxine (treated); the third group was not infected. Small volumes of blood were collected for the determination of parasitaemia and packed cell volume (PCV). The concentrations of T3 and T4 were measured in plasma by radioimmunoassay. The decrease in PCV correlated closely (y = -0.38x + 15.2; r = 0.82

OLE PROFMALOIYGEOFFREYM. "LOMHOLT, J.P., JOHANSEN, K and MALOIY, G.M.O. (1975) Is aestivating lungfish the first vertebrate with suctional breathing? Nature London 257, 787-788.". In: Proceedings of the 7th Pan-African Ornithological Congress, p. 17. EAMJ; 1975. Abstract
Serum acid phosphatase was measured in patients with enlarged benign and malignant prostate before and after rectal examination. Amongst the patients with benign glands, rectal examination did not produce any significant false elevation of the enzyme. Rectal examination, however, caused a rise in the enzyme level in a few untreated cancer patients and in cancer patients who has become refractory to hormonal therapy. This rise would help rather than mislead in the diagnosis of malignant prostate and also in the identifying treated patients who had become refractory to treatment. Thus, when serum acid phosphatase is properly determined, elevated levels should always arouse suspicion of malignant prostate or other lesions associated with high enzyme level even is such determination was preceded by rectal examination. There appears to be no merit in the teaching that the determination of serum acid phosphatase should be delayed after rectal examination.
JAMES PROFODEK. ""Lome IV: Dynamic or Static Improvement on the Previous conventions? The Sub-Saharan Africa Perspective" 27 Comparative and International Law Journal of Southern Africa at 164-198.". In: Paper presented at the WIPO High Level Forum on IP Policy and Strategy, Tokyo . Prof. James Otieno-Odek; 1994. Abstract
J. O. Midiwo, A. Yenesew, B. F. Juma, S. Dereses, J. A. Ayoo, A. Aluoch and S. Guchu There are several described medicinal plants in Kenya from a flora of approximately 10,000 members. Strong cross-medical information from the 42 ethnic groups points to the high potential of some of these species. The Myrsinaceae are well established ethno-anthelmintics and anti-bacterials. They are harbingers of long alkyl side chain benzoquinones which clearly have a protective function from their histochemical disposition. The main benzoquinone in the sub-family Myrsinodae is embelin while for the Maesodae it is maesaquinone together with its 5-acetyl derivative; the distribution of these benzoquinones by their alkyl side chain length or the presence/absence of a 6-methyl group is in accord with morphological sub-family de-limitation. The benzoquinones showed anti-feedant, anti-microbial, phytotoxic, acaricidal, insecticidal and nematicidal activity. Many other benzoquinones of medium and minor concentration were also isolated and characterised. Some plants belonging to the Polygonaceae which are widely used as ethno-anthelmintics have been studied. The common anthelmintic anthraquinones were obtained from all five Rumex species while the naphthalenic acetogenin derivative, nepodin was more selectively distributed. The leaf of Polygonum senegalense is up to 17% surface exudate; about thirteen non polar flavonoid derivatives (chalcones, dihydrochalcones, flavanones and a flavone) have been isolated from it. From the internal aerial tissues of this plant, the major flavonoids were common flavonoids, quercetin, kaempferol, luteolin and their glycosides. The only unique compound isolated from this plant was 2prime-glucosyl-6prime-hydroxy-4prime-methoxydihydrochalcone whose aglycone, uvangolatin is part of the exudate mixture. Other leaf exudate plants studied include the stomach-ache medicine, Psiadia punctulata (Compositae) from which novel methylated flavonoids, kaurene and trachyloban diterpenes have been found
JAMES PROFODEK. "The Lome Conventions (1975-2000): Background to the Trade Relationship Between Africa-Caribbean-Pacific Countries and the European Economic Communities.". In: Paper presented at the WIPO High Level Forum on IP Policy and Strategy, Tokyo . Prof. James Otieno-Odek; 2005. Abstract
J. O. Midiwo, A. Yenesew, B. F. Juma, S. Dereses, J. A. Ayoo, A. Aluoch and S. Guchu There are several described medicinal plants in Kenya from a flora of approximately 10,000 members. Strong cross-medical information from the 42 ethnic groups points to the high potential of some of these species. The Myrsinaceae are well established ethno-anthelmintics and anti-bacterials. They are harbingers of long alkyl side chain benzoquinones which clearly have a protective function from their histochemical disposition. The main benzoquinone in the sub-family Myrsinodae is embelin while for the Maesodae it is maesaquinone together with its 5-acetyl derivative; the distribution of these benzoquinones by their alkyl side chain length or the presence/absence of a 6-methyl group is in accord with morphological sub-family de-limitation. The benzoquinones showed anti-feedant, anti-microbial, phytotoxic, acaricidal, insecticidal and nematicidal activity. Many other benzoquinones of medium and minor concentration were also isolated and characterised. Some plants belonging to the Polygonaceae which are widely used as ethno-anthelmintics have been studied. The common anthelmintic anthraquinones were obtained from all five Rumex species while the naphthalenic acetogenin derivative, nepodin was more selectively distributed. The leaf of Polygonum senegalense is up to 17% surface exudate; about thirteen non polar flavonoid derivatives (chalcones, dihydrochalcones, flavanones and a flavone) have been isolated from it. From the internal aerial tissues of this plant, the major flavonoids were common flavonoids, quercetin, kaempferol, luteolin and their glycosides. The only unique compound isolated from this plant was 2prime-glucosyl-6prime-hydroxy-4prime-methoxydihydrochalcone whose aglycone, uvangolatin is part of the exudate mixture. Other leaf exudate plants studied include the stomach-ache medicine, Psiadia punctulata (Compositae) from which novel methylated flavonoids, kaurene and trachyloban diterpenes have been found
MBORI- PROFNGACHADOROTHYA, ELIZABETH DROBIMBO. "Lohman B, Slyker J, Mbori-Ngacha D, Bosire R, Farquhar C, Obimbo E., Otieno P, Nduati R, Rowland-Jones S, John-Stewart G. Prevalence and Magnitude of HIV-1-specific Lymphocyte Responses in Breast Milk from HIV-1 Seropositive Women. J. Infect Dis. Dec 2003.". In: J. Infect Dis. Dec 2003; 1888 (11) 1999-74. Kisipan, M.L.; 2003. Abstract
Department of Medical Microbiology, University of Nairobi, Kenya. Human immunodeficiency virus (HIV) type 1-specific cell-mediated immunity of breast milk may influence the likelihood of mother-to-child transmission of HIV-1 via breast-feeding. In breast-milk specimens collected during the first month postpartum from HIV-1-seropositive women in Nairobi, HIV-1 gag-specific cellular responses were detected in 17 (47%) of 36, and env-specific cellular responses were present in 20 (40%) of 50. Peripheral blood lymphocyte responses against either gag or env were detected in 35 (66%) of the 53 subjects, 18 (51%) of whom had positive gag or env responses in their breast milk. In paired analyses of blood and breast milk, the mean magnitude of responses to env or gag stimulation in breast milk was significantly higher than that in blood and remained higher in breast milk after normalization of responses according to CD8+ lymphocyte count. These results suggest that CD8+ lymphocytes present in breast milk have the capacity to recognize HIV-1-infected cells and may be selectively transported to breast milk to reduce either viral replication or transmission in breast milk.
MBORI- PROFNGACHADOROTHYA, ELIZABETH DROBIMBO. "Lohman B, Slyker J, Mbori-Ngacha D, Bosire R, Farquhar C, Obimbo E., Otieno P, Nduati R, Rowland-Jones S, John-Stewart G. Prevalence and Magnitude of HIV-1-specific Lymphocyte Responses in Breast Milk from HIV-1 Seropositive Women. J. Infect Dis. Dec 2003.". In: J. Infect Dis. Dec 2003; 1888 (11) 1999-74. Earthscan, London. 978-1-84407-469-3 (*); 2003. Abstract
Department of Medical Microbiology, University of Nairobi, Kenya. Human immunodeficiency virus (HIV) type 1-specific cell-mediated immunity of breast milk may influence the likelihood of mother-to-child transmission of HIV-1 via breast-feeding. In breast-milk specimens collected during the first month postpartum from HIV-1-seropositive women in Nairobi, HIV-1 gag-specific cellular responses were detected in 17 (47%) of 36, and env-specific cellular responses were present in 20 (40%) of 50. Peripheral blood lymphocyte responses against either gag or env were detected in 35 (66%) of the 53 subjects, 18 (51%) of whom had positive gag or env responses in their breast milk. In paired analyses of blood and breast milk, the mean magnitude of responses to env or gag stimulation in breast milk was significantly higher than that in blood and remained higher in breast milk after normalization of responses according to CD8+ lymphocyte count. These results suggest that CD8+ lymphocytes present in breast milk have the capacity to recognize HIV-1-infected cells and may be selectively transported to breast milk to reduce either viral replication or transmission in breast milk.
MBORI- PROFNGACHADOROTHYA, ELIZABETH DROBIMBO. "Lohman B, Slyker J, Mbori-Ngacha D, Bosire R, Farquhar C, Obimbo E, Otieno P, Nduati R, Rowland-Jones S, John-Stewart G. Prevalence and Magnitude of HIV-1-specific Lymphocyte Responses in Breast Milk from HIV-1 Seropositive Women. J Infect Dis. 2003:188:1.". In: J Infect Dis. 2003:188:1666-1674. Kisipan, M.L.; 2003. Abstract
Department of Medical Microbiology, University of Nairobi, Kenya. Human immunodeficiency virus (HIV) type 1-specific cell-mediated immunity of breast milk may influence the likelihood of mother-to-child transmission of HIV-1 via breast-feeding. In breast-milk specimens collected during the first month postpartum from HIV-1-seropositive women in Nairobi, HIV-1 gag-specific cellular responses were detected in 17 (47%) of 36, and env-specific cellular responses were present in 20 (40%) of 50. Peripheral blood lymphocyte responses against either gag or env were detected in 35 (66%) of the 53 subjects, 18 (51%) of whom had positive gag or env responses in their breast milk. In paired analyses of blood and breast milk, the mean magnitude of responses to env or gag stimulation in breast milk was significantly higher than that in blood and remained higher in breast milk after normalization of responses according to CD8+ lymphocyte count. These results suggest that CD8+ lymphocytes present in breast milk have the capacity to recognize HIV-1-infected cells and may be selectively transported to breast milk to reduce either viral replication or transmission in breast milk.
MBORI- PROFNGACHADOROTHYA, ELIZABETH DROBIMBO. "Lohman B, Slyker J, Mbori-Ngacha D, Bosire R, Farquhar C, Obimbo E, Otieno P, Nduati R, Rowland-Jones S, John-Stewart G. Prevalence and Magnitude of HIV-1-specific Lymphocyte Responses in Breast Milk from HIV-1 Seropositive Women. J Infect Dis. 2003:188:1.". In: J Infect Dis. 2003:188:1666-1674. Earthscan, London. 978-1-84407-469-3 (*); 2003. Abstract
Department of Medical Microbiology, University of Nairobi, Kenya. Human immunodeficiency virus (HIV) type 1-specific cell-mediated immunity of breast milk may influence the likelihood of mother-to-child transmission of HIV-1 via breast-feeding. In breast-milk specimens collected during the first month postpartum from HIV-1-seropositive women in Nairobi, HIV-1 gag-specific cellular responses were detected in 17 (47%) of 36, and env-specific cellular responses were present in 20 (40%) of 50. Peripheral blood lymphocyte responses against either gag or env were detected in 35 (66%) of the 53 subjects, 18 (51%) of whom had positive gag or env responses in their breast milk. In paired analyses of blood and breast milk, the mean magnitude of responses to env or gag stimulation in breast milk was significantly higher than that in blood and remained higher in breast milk after normalization of responses according to CD8+ lymphocyte count. These results suggest that CD8+ lymphocytes present in breast milk have the capacity to recognize HIV-1-infected cells and may be selectively transported to breast milk to reduce either viral replication or transmission in breast milk.
BUURI MRKABURIAMARTIN, BUURI MRKABURIAMARTIN. "Logistic regression modeling of poverty using Demographic and Health Survey data.". In: European Journal of Social Science. John Benjamins Publishing Company; 2010. Abstract
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O DRACHIATHOMASN. "Logistic regression modeling of poverty using Demographic and Health Survey data.". In: European Journal of Social Science. John Benjamins Publishing Company; 2010. Abstract
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MWANGI MRMUREITHISTEPHEN. "Logistic models with rainfall generated carrying capacities for wild herbivores. Kinyua, P.I.D. and Mureithi, S.M. Paper Presented at: The 19th East African Environmental Network Conference 22nd -23rd May 2009, AMREF International Training Centre, Nairobi.". In: The 19th East African Environmental Network Conference 22nd -23rd May 2009, AMREF International Training Centre, Nairobi Kenya. Conference theme: Energy, Gender and the Environment. EAEN; 2009. Abstract
Cities around the world are currently facing considerable pressure to cope with infrastructure provision amidst rapid urban development and economic growth. Kenya is also facing an increasing growth of informal settlements in her urban centres. As rapid urbanization takes its toll, so has the development and growth of slums. More than 34% of Kenya‟s total population lives in urban areas and of this, more than 71% is confined in informal settlements (UN-Habitat, 2009). This paper presents the result of study to assess the effects of the change in land use in two sub-catchments, in an urban area close to the Nairobi Central Business District (NCBD). The paper compares the change in land use of two regions in Nairobi, Kenya that are slowly changing due to the fast growing population and economy, on urban runoff.
Johnson L, Onjala J. "Logic of the Belt and Road Initiatives Early Eastern Africa Node: Economic, Demographic and Political Economy Rationales." Journal of Chinese Economic and Foreign Trade Studies,. 2021.
O PROFMCLIGEYOSETH. "Lodenyo HA, McLigeyo SO, Ogola EN.Cardiovascular disease in elderly in-patients at the Kenyatta National Hospital, Nairobi-Kenya. East Afr Med J. 1997 Oct;74(10):647-51.". In: East African Medical Journal. 74(10):605-606, 1997. University of Nairobi.; 1997. Abstract
A prospective study to determine the prevalence and profile of cardiovascular disease in elderly patients admitted into the medical wards, Kenyatta National Hospital, was carried out between July 1991 and January 1992. Two hundred and two patients over 60 years of age were admitted into the medical wards over this period. This formed seven per cent of the total medical admissions. Two of these refused to take part in the study. Of the 200 elderly patients evaluated for cardiovascular disease, 146 (73%) were between 60 and 75 years of age with only 26 (13%) being over 85 years. Fifty seven per cent were males. Clinical evidence of cardiovascular disease was present in 79 (39.5%) of the patients evaluated. There was no sex difference in the prevalence of cardiovascular disease as judged from clinical evaluation (37.7% males versus 41.9% females, p > 0.05). Cardiovascular diseases in our medical in-patients at Kenyatta National Hospital are common and especially so with hypertension which plays an important role in the aetiology of congestive heart failure and cerebravascular accidents. Cardiac arrhythmias are also common though not necessarily symptomatic. Rheumatic heart disease and cardiomyopathies were uncommon in our study population. A community-based survey is needed to determine the true prevalence of cardiovascular diseases in the elderly and their contribution to morbidity in this sector of the population.
N PROFOGOLAELIJAHS. "Lodenyo H.A, McLigeyo S.O OGOLA E.N.Cardiovascular disease in elderly patients at the Kenyatta national hospital. East Africa. J.741: 647,1997.". In: East Africa. J.741: 647,1997. Kisipan, M.L.; 1997. Abstract
A prospective study to determine the prevalence and profile of cardiovascular disease in elderly patients admitted into the medical wards, Kenyatta National Hospital, was carried out between July 1991 and January 1992. Two hundred and two patients over 60 years of age were admitted into the medical wards over this period. This formed seven per cent of the total medical admissions. Two of these refused to take part in the study. Of the 200 elderly patients evaluated for cardiovascular disease, 146 (73%) were between 60 and 75 years of age with only 26 (13%) being over 85 years. Fifty seven per cent were males. Clinical evidence of cardiovascular disease was present in 79 (39.5%) of the patients evaluated. There was no sex difference in the prevalence of cardiovascular disease as judged from clinical evaluation (37.7% males versus 41.9% females, p > 0.05). Cardiovascular diseases in our medical in-patients at Kenyatta National Hospital are common and especially so with hypertension which plays an important role in the aetiology of congestive heart failure and cerebravascular accidents. Cardiac arrhythmias are also common though not necessarily symptomatic. Rheumatic heart disease and cardiomyopathies were uncommon in our study population. A community-based survey is needed to determine the true prevalence of cardiovascular diseases in the elderly and their contribution to morbidity in this sector of the population.
Alexander RM;, Maloiy GMO. "Locomotion of African mammals."; 1989.
Munyiva NMM. Location-based multilingual mobile phone browser case: Agricultural Information System Access. Odongo PO, ed.; 2008. Abstract

There has been a drastic growth in mobile phone usage in Kenya over the past few years .In June of 1999, Kenya had 15,000 mobile phone subscribers. By the end of 2004 the country had 3.4 million subscribers, and by mid-2005 the number was estimated to be over 4 million. [2]In addition prices of {WAP}-enabled smart phones have declined drastically over the recent past. There is an immense amount of agricultural information in Kenya that has been acquired by various research institutions and Non Government organizations. This information is largely in form of books, journals and articles and only limited static information is available on the web sites. In addition there is no single website that captures all information relating to Agricultural production that is required by farmers in a user friendly, location and language localized manner. Agricultural information applications developed that utilize mobile phone technology are mainly Short Message Service ({SMS}) based which do not take advantage of the full capabilities of phones available in the market. This project sets out to develop a system that will facilitate access to Agricultural information on the internet via mobile phones through creation of an interface that is location based meaning it determines the geographical position of a user and Multilingual that is there are various choices of languages that can be used to access the system In addition it sets out to merge all information required by farmers for Agricultural production into one Agricultural information system site. Display of required information will be based on geographical location of the user. The system utilizes {WAP} technology for the web interface and J2ME technology for the mobile phone interface using the {GSM} network to acquire location information. All the theory, rnethodology and implementation of the system are highlighted and illustrated inthis project report.

Mwachaka PM, Saidi HS, Odula PO, Awori KO, Kaisha WO. "Locating the arcuate line of {Douglas}: is it of surgical relevance?" Clinical anatomy (New York, N.Y.). 2010;23:84-86. Abstract

Ventral hernia formation is a common complication of rectus abdominis musculocutaneous flap harvest. The site and extent of harvest of the flap are known contributing factors. Therefore, an accurate location of the arcuate line of Douglas, which marks the lower extent of the posterior wall of the rectus sheath, may be relevant before harvesting the flap. This study is aimed at determining the position of the arcuate line in relation to anatomical landmarks of the anterior abdominal wall. Arcuate lines were examined in 80 (44 male, 36 female) subjects, aged between 18 and 70 years, during autopsies and dissection. The position of the arcuate line was determined in relation to the umbilicus, pubic symphysis, and intersections of rectus abdominis muscle. Sixty four (80.4%) cases had the arcuate line. In most cases (52), this line was located in the upper half of a line between the umbilicus and the pubic symphysis. Most males (93%) had the arcuate line, while more than a third of females did not have it. In all these cases, the line occurred bilaterally as a single arcade, constantly at the most distal intersection of the rectus abdominis muscle. Consequently, the arcuate line is most reliably marked superficially by the distal tendinous intersection of the rectus abdominis muscle. Harvesting of the muscle cranial to this point will minimize defects in the anterior abdominal wall that may lead to hernia formation.

Mwachaka PM, Saidi HS, Odula PO, Awori KO, Kaisha WO. "Locating the arcuate line of {Douglas}: is it of surgical relevance?" Clinical anatomy (New York, N.Y.). 2010;23:84-86. Abstract

Ventral hernia formation is a common complication of rectus abdominis musculocutaneous flap harvest. The site and extent of harvest of the flap are known contributing factors. Therefore, an accurate location of the arcuate line of Douglas, which marks the lower extent of the posterior wall of the rectus sheath, may be relevant before harvesting the flap. This study is aimed at determining the position of the arcuate line in relation to anatomical landmarks of the anterior abdominal wall. Arcuate lines were examined in 80 (44 male, 36 female) subjects, aged between 18 and 70 years, during autopsies and dissection. The position of the arcuate line was determined in relation to the umbilicus, pubic symphysis, and intersections of rectus abdominis muscle. Sixty four (80.4%) cases had the arcuate line. In most cases (52), this line was located in the upper half of a line between the umbilicus and the pubic symphysis. Most males (93%) had the arcuate line, while more than a third of females did not have it. In all these cases, the line occurred bilaterally as a single arcade, constantly at the most distal intersection of the rectus abdominis muscle. Consequently, the arcuate line is most reliably marked superficially by the distal tendinous intersection of the rectus abdominis muscle. Harvesting of the muscle cranial to this point will minimize defects in the anterior abdominal wall that may lead to hernia formation.

HASSAN PROFSAIDI. "Locating the arcuate line of Douglas: Is it of surgical significance? Mwachaka P.M., Saidi H., Odula P.O., Awori K.O., Kaisha W.O. Clinical Anatomy 2010; 23: 84-86.". In: Clinical Anatomy. Surgical society of Kenya; 2010. Abstract
14.00 800x600 Normal 0 false false false EN-GB X-NONE X-NONE MicrosoftInternetExplorer4 /* Style Definitions */ table.MsoNormalTable {mso-style-name:"Table Normal"; mso-tstyle-rowband-size:0; mso-tstyle-colband-size:0; mso-style-noshow:yes; mso-style-priority:99; mso-style-parent:""; mso-padding-alt:0cm 5.4pt 0cm 5.4pt; mso-para-margin:0cm; mso-para-margin-bottom:.0001pt; mso-pagination:widow-orphan; font-size:10.0pt; font-family:"Calibri","sans-serif"; mso-bidi-font-family:"Times New Roman";} Variant anatomy of the superior thyroid artery is important during surgical procedures, interpretation of angiograms, and interventional radiography in the neck. Pattern of the variations shows population differences but there is no data from the Kenyan population. This study therefore investigated the variations in origin of the superior thyroid artery in a Kenyan population. Forty six necks (36 males and 10 females) from 46 cadavers of black Kenyans in Department of Human Anatomy University of Nairobi, Kenya were bilaterally dissected to expose the origin of the superior thyroid artery. Pattern of origin of the vessel was determined on both sides in males and females. It originated from the external carotid artery common carotid artery and linguo-facial trunk in 80%, 13% and 6.5% of the cadavers respectively on the right side. All but one of the superior thyroid arteries were ventral branches. There was asymmetric origin in 6.5% of cases. Origin from the common carotid artery was associated with high carotid bifurcation. Nearly 20% of superior thyroid arteries showed variant origin. Of these, 6.5% arose from the linguo-facial trunk, much higher than in the Caucasian and Oriental populations. Origin from common carotid artery is substantially lower than prevailing figures from other populations. These findings support ethnic variations. Preoperative angiographic evaluation is recommended.
KAISHA DROTSIANYIWYCKLIFFE. "Locating the arcuate line of douglas: is it of surgical relevance?". In: Int. J. Morphol. Wiley; 2010.

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