DNA Microsatellite Analysis of Kenyan Isolates of Rhizoctonia solani Author: A.W. Mwang`Ombe, G. Thiong`O, F.M. Olubayo and E.K. Kiprop

Citation:
W. PROFMWANGOMBEAGNES. "DNA Microsatellite Analysis of Kenyan Isolates of Rhizoctonia solani Author: A.W. Mwang`Ombe, G. Thiong`O, F.M. Olubayo and E.K. Kiprop.". In: Plant Pathology Journal - 62-PPJ-2K6 -2006. J Hum Ecol, 26(3): 163-173 (2009).; 2006.

Abstract:

Seven hundred and fifty bean plant samples with root rot symptoms were

collected from farmers' fields during two surreys carried out in Embu district,

Kenya. Various fungal pathogens were isolated in the laboratory from these

samples; among them were 50 isolates of Rhizoctonia solani, which were

subjected to pathogenicity tests in a glasshouse. Thirty-six isolates of R. solani

obtained from beans with root rots were subjected to DNA microsatellite analysis.

Five isolates of R. solani that cause black scarf of potatoes (Solanum tuberosum

L.) were also analysed alongside those from the beans. A total of 50 alleles were

detected when six microsatellite loci were typed in the 41 samples, with the mean

of 8.33 and a range of 3 at locus RB23 to 19 at locus AF513014. The smallest

allele size was 129 basepair at locus RE102 and the largest was 297 basepair at

locus AY212027. Microsatellite analysis showed a moderate variation among the

isolates from different agro-ecological zones and administrative boundaries

(divisions). Phylogenetic analysis revealed 3 major clusters within the population

of 41 isolates of R. solani from Kenya. Clusters 1, 2 and 3 had 15, 10 and 75%

isolates, respectively. However, cluster 3 had 4 sub-clusters and cluster 1 had 2

sub-clusters, while cluster 2 did not have a sub-cluster. There was no relationship

between microsatellites and geographical origin of the isolates. This is the first

study on the genetic diversity of R. solani using DNA microsatellite analysis in

Kenya.

Notes:

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