Bio

BIOGRAPHY – PROF. EUNICE WANJIRU MUTITU

(B.Sc., Agric., M.Sc., PhD University of Nairobi)

Prof. Eunice Mutitu is a full professor of the Department of Plant Science and Crop Protection and is currently the Director of Board of Post graduate Studies of the University of Nairobi. She previously served as the Chairman of the Faculty of Agriculture Post graduate studies committee and the founding Chairman of the Department of Crop Protection. She has served the University of Nairobi for several years where she is an active member of staff.

Her Research interests include:

Publications


2012

W., MUTHOMIJ, K. MUREITHIB, N. CHEMINING'WAG, K. GATHUMBIJ, W. PROFMUTITUEUNICE.  2012.  Aspergillus species and Aflatoxin b1 in soil, maize grain and flour samples from semi-arid and humid regions of Kenya.. International Journal of AgriScience . 2(1):22-34.

2011

2010

M., MW, B. KOOPMAN, V. TIEDEMMANNA, W. PROFMUTITUEUNICE, W. DRKIMENJUJOHN.  2010.  Evaluation of genetic variability of Kenyan, German and Austrian isolates of Exserohilum turcicum using Amplified Fragment Length Polymorphism DNA marker.. Biotechnology. 9(2):204-211.
M., MW, B. KOOPMAN, V. TIEDEMMANNA, W. PROFMUTITUEUNICE, W. DRKIMENJUJOHN.  2010.  Race typing and evaluation of aggressiveness of Exserohilum turcicum isolates of Kenyan German and Austrian origin.. World Journal of Agricultural Sciences . 6(3):277-284.
M., MW, B. KOOPMAN, V. TIEDEMMANNA, W. PROFMUTITUEUNICE, W. DRKIMENJUJOHN.  2010.  Use of Repetitive Extragenic Palndromic (REP), Enterobacterial Repetitive Intergenic Consensus (ERIC) and BOX sequences to fingerprint Exserohilum turcicum isolates.. Journal of Applied Biosciences. 30:1828-1838.

2009

Gichuru, EK;, Combes MC;, Mutitu EW;, Ngugi ECK;, Omondi CO;, Bertrand B;, Lashermes P.  2009.  Towards the development of sequence based markers for resistance to coffee berry disease (Colletotrichum kahawae). AbstractWebsite

Coffee Berry Disease which affects green Arabica coffee (Coffea arabica) berries is caused by the fungus Colletotrichum kahawae and is a major problem in Arabica coffee production in African countries. Breeding for resistance to this disease is therefore to a major priority in these countries avoid intensive chemical usage for its control. Recently, microsatellite and Amplified Fragment Length Polymorphisms (AFLP) markers for a gene conferring resistance to the disease were identified and mapped onto the chromosomal region carrying the gene. To improve the repeatability of the AFLP markers, four of the marker bands were selected for cloning and sequencing to facilitate specific primers to be designed. Three of the resultant primers did not amplify products that exhibited polymorphism characteristic of the parent AFLP bands; but one primer pair amplified a product that dominantly identified the presence of the parent AFLP marker at an optimum temperature of 62°C followed by electrophoresis in agarose. The reliability of the designed primers was confirmed by analysis in 95 plants from a F2 population previously used to map the chromosomal fragment carrying the resistance. The importance of the results in enhancing the utility of the parent AFLP marker in relation to analytical costs and position on the chromosomal fragment is discussed.

N., MAKUMBABA, B. MF, W. PROFMUTITUEUNICE.  2009.  In vitro and in vivo tests of Bacillus licheniformis MGrP1 antibiotics culture filtrate as a potential biocontrol agent against bean anthracnose.. East African Journal of Pure and Applied Science . 2:1-16.
W., DRKIMENJUJOHN, U. ODEROGO, W. PROFMUTITUEUNICE, M. WACHIRAP, D. NARLAR, M. MW.  2009.  Suitability of locally available substrates for oyster mushroom (Pleurotusostreatus). 8(7):510-514.
M., MW, W. PROFMUTITUEUNICE, MUNGE PROFMUKUNYAD.  2009.  Isolation and screening of actinomycete isolates for antagonistic activity against plant pathogens. Botswana Journal of Agriculture and Applied Sciences. 5(2):73-81.
O., MAGOMERET, D. OBUKOSIAS, W. PROFMUTITUEUNICE, O. OLUBAYOF, C. NGICHABE, I. SHIBAIROS.  2009.  PCR detection and distribution of huanglognbing disease and psyllid vectors on citrus varieties with changes in elevation in Kenya.. Journal of Biological Sciences . 9(7):697-709.
O., MAGOMERET, D. OBUKOSIAS, W. PROFMUTITUEUNICE, F O, C. NGICHABE, I. SHIBAIROS.  2009.  Molecular Characterization of ‘Candidatus’ liberibacter species/strains causing huanglongbing disease of citrus in Kenya.. http://www.ejbiotechnology.info/content/vol 12/issue 2/full/2/.

2008

W., PROFMUTITUEUNICE, M. WAGACHAJ, W. MUTHOMIJ, B. MF.  2008.  Control of bean rust on snap beans (Phaseolus vulgaris l.) using antibiotic metaboites produced by Bacillus and Streptomyces species. . Botswana Journal of Agriculture and Applied Sciences . 4(1):62-69.
W., PROFMUTITUEUNICE, MUNGE PROFMUKUNYAD, M. MW.  2008.  Evaluation of Antibiotic Metabolites from Actinomycetes Isolates for the Control of Late Blight of Tomatoes under Greenhouse Conditions. . Asian Journal of Plant Sciences . 7(3):284-290.
W, DRMUTHOMIJAMES, K. NDUNGUJ, K. GATHUMBIJ, M. WAGACHAJ, W. PROFMUTITUEUNICE.  2008.  The occurrence of Fusarium species and mycotoxins in Kenyan wheat.. Crop Protection . 27(8):1215-1219.
E., GICHURE, O. AGWANDAC, C. COMBESM, W. PROFMUTITUEUNICE, K. NGUGIEC, B. BERCRAND, P. LASHERMES.  2008.  Identification of Molecular Markers linked to a gene conferring resistance to coffee berry disease (Colletotrichum Kahawae in Coffee arabica..
M., MW, W. PROFMUTITUEUNICE, MUNGE PROFMUKUNYAD.  2008.  Identification of Selected Actinomycete Isolates and Characterization of Their Antibiotic Metabolites.. Journal of Biological Sciences . 8(6):1021-1026.
M., MW, W. PROFMUTITUEUNICE, W. DRKIMENJUJOHN, B. KOOPMAN, V. TIEDEMMANNA.  2008.  Infectious Structures and Response of Maize Plants to Invasion by Exserohilum turcicum (Pass). in compatible and incompatible host pathogen systems.. Journal of Applied Biosciences (2008). Vol. 10(2):532-537.
N., MWEKEA, W. DRKIMENJUJOHN, A. SEIFA, K. MUTUAG, W. PROFMUTITUEUNICE.  2008.  Potential of Sequential Cropping in the Management of Root-Knot Nematodes in Okra.. Asian Journal of Plant Sciences . 7(4):399-403.
M., MW, B. KOOPMAN, V. TIEDEMMANNA, W. PROFMUTITUEUNICE, W. DRKIMENJUJOHN.  2008.  A review of the amplified fragment length polymorphism (AFLP) technique in genotyping and DNA fingerprinting studies.. Journal of applied Biosciences, Vol. 9(2): 389-395.

2007

Wakahiu, MW, Gildemacher PR, Kinyua ZM, Kabira JN, Kimenju AW, Mutitu EW.  2007.  Occurrence of potato bacterial wilt caused by Ralstonia solanacearum in Kenya and opportunities for intervention.
M., MW, W. PROFMUTITUEUNICE, W. DRKIMENJUJOHN.  2007.  Reaction of Some Kenyan Maize Genotypes to Turcicum Leaf Blight Under Greenhouse and Field Conditions.. Asian Journal of Plant Sciences 6 (8): 1190-1196.
M., MW, W. PROFMUTITUEUNICE, MUNGE PROFMUKUNYAD.  2007.  Characterization of antibiotic metabolites from actinomycete isolates.. African Crop Science Conference Proceedings Vol. 8:. :2103-2107.

2004

Miano, DW;, Kimenju JW;, Mutitu EW;, Waudo SW;, Samson JM.  2004.  Management of Root-knob Nematodes(meloidogyne ssp)using organic amendments.
W., PROFMUTITUEUNICE.  2004.  Appropriate conditions for handling grain on the farm and their effect on grain quality.. Grain milling stakeholders workshop . : University of Nairobi Case, in the proceedings of the IST-Africa 2008 Conference; Windhoek, Namibia Abstract
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2003

Otipa, MJ;, Kimenju JW;, Mutitu EW;, Karanja NK.  2003.  Potential rotation crops and cropping cycles for root-knot (Meloidogyne spp.) nematode control in tomato. Abstract

Tomato is attacked by several plant parasitic nematodes but root-knot nematodes are the most devastating and cause considerable losses in Kenya. Studies were undertaken under greenhouse and field conditions to determine the suppressiveness of a wide range of plant species to root-knot (Meloidogyne spp.) nematodes. Potted plants were inoculated with 6000 eggs and /or juveniles while the field experiments were in nematode infested fields. Among the plants tested, Tagetes patula, Gossypium hirsutum, Desmodium uncinatum, Chloris gayana, Zea mays, Alstroemeria sp., Capsicum annuum, Crotalaria juncea, Arachis hypogaea, Sorghum bicolor, Tithonia diversifolia and Pennisetum purpureum were rated as poor hosts with galling and egg mass indices ranging from 0 to 3. High galling and egg mass indices ranging from 7-9 were recorded on Lablab purpureus, Coriandum, sativum, Statice sp., Brassica oleracea var. gloria, Helianthus annuus, Vigna subterranea while Muguna pruriens, Lactuca sativa, Allium ampeloprasum, Sesamum indicum, Allium cepa, Onnis sp., Brassica Oleracea Var. chinensis, Asparagus sp., Brassica oleracea var. botrytis, Ornithogolum arabicum. Tuberose sp. and Chrysanthemum indicum, were rated moderately resistant with galling and egg mass indices ranging from 3 to 6. Damage by nematodes was significantly (P=0.05) reduced in tomato planted after sweetcorn or in sweetcorn with Tagetes patula, Crotalaria juncea, Sorghum bicolor and Asparagus sp. in the field. This study shows that despite the fact that Meloidogyne spp. have wide host ranges, there is a wide range of economically important plants from which suitable candidates can be selected for use as rotation or interplants in their management.

Muthomi, JW;, Mutitu EW.  2003.  Occurrence of mycotoxin producing Fusarium species and other fungi on wheat kernels harvest in selected districts of Kenya. Abstract

Wheat samples collected from 5 wheat growing districts of Kenya were investigated for contamination by different fungi. Kernels were plated on agar media and the fungi that grew were identified by cultural and morphological characteristics to genus level. Fusarium isolates were identified to species level and isolates of F. graminearum were tested for mycotoxin production in culture. The major genera of fungi isolated according to decreasing frequency were Epicoccum (52.8%), Alternaria (34%), Fusarium (6%), Aspergillus (2.3%) and Penicillium (1.8%). The frequently isolated Epicoccum species was identified as E. purpurascenes. Cladosporium and Rhizopus spp. were also isolated at very low frequencies. The most frequently isolated Fusarium species were F. poae (43%), F. graminearum (39%), and F. avenaceum (8%). Other Fusarium species isolated were F. equiseti, F. oxysporum, F. camptoceras and F. chlamydosporium. Most isolates of F. graminearum produced mycotoxin deoxynivalenol and zearalenone. The isolated Fusarium species are known to cause head blight in wheat resulting in mycotoxin contamination of the grains. The results therefore indicated that head blight is widely distributed at low levels in the wheat growing areas investigated. This inoculum is potentially capable of producing severe infections under optimum weather conditions.

W., PROFMUTITUEUNICE.  2003.  Causes of ear rot of maize with mycotoxin implications in Eastern and Central Kenya. African Crop Science Conference. : University of Nairobi Case, in the proceedings of the IST-Africa 2008 Conference; Windhoek, Namibia Abstract
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W., PROFMUTITUEUNICE.  2003.  field management of late blight of tomatoes caused by Phytophthora infestans using antibiotics from Streptomyces spp.. African Crop Science Conference. : University of Nairobi Case, in the proceedings of the IST-Africa 2008 Conference; Windhoek, Namibia Abstract
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2002

Muthomi, JW, Orke EC, Dehne HW, Mutitu EW.  2002.  Susceptibility of Kenyan wheat varieties to head blight, fungal invation and Deoxynivalenol accumulation inoculated with Fusarium graminearum. Abstract

Fifteen wheat varieties commercially grown in Kenya were tested for their susceptibility to head blight and mycotoxin accumulation after inoculation with Fusarium gramineanuu in pot experiments. The strain or the pathogen used had been isolated from wheat collected in different growing areas or Kenya. Head blight suscep¬tibility was assessed as the percentage or spikclcts bleached and area under disease progress curve: kernel colonization by fungal mycelium was determined as ergosterol content. All varieties were round to be moderately to highly susceptible. However. the varieties differed in head blight susceptibility (29-68% or spike¬lets bleached; mean 54%). fungal colonization (67- 187 I'g g ergosterol content: mean III Ilg g) and the resulting mycotoxin contamination [deoxynivalenol (DON) 5-31 JIg g: mean 13.5 I'g g], Grain weight reductions due to head blight ranged from 23 to 57% (mean 44%). The varieties could be therefore divided into partially resistant and highly susceptible genotypes. The kernels or highly susceptible varieties had higher mycotoxin and ergosterol contents. However. the ker¬nels or some varieties contained more fungal mycelium (ergosterol) without the corresponding high amounts or DON. suggesting that they possess some resistance to DON accumulation. Less susceptible varieties showed resistance LO fungal spread. as indicated by a slow disease development and lower content or fungal biomass.

  2002.  Challenges to Organic Farming and Sustainable Land Use. Department of Crop Protection.
W., PROFMUTITUEUNICE.  2002.  Variation among Fusarium species and isolated infecting wheat ears based on aggressiveness, mycotoxin production and RAPD . African Crop Science Conference. : University of Nairobi Case, in the proceedings of the IST-Africa 2008 Conference; Windhoek, Namibia Abstract
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2001

Kimani, EWN;, Mutitu EW;, Waudo SW;, Obukosia SD;, Kimani PM;, Ikahu JM;, Waithaka K.  2001.  Symptoms, causal agent and distribution of wilt disease of pyrethrum (Chrysanthemum cinerariafolium Vis) in Kenya.

2000

W., PROFMUTITUEUNICE.  2000.  Characterization of Fusarium culmorum isolates by mycotoxin production and aggressiveness to winter wheat. Journal of plant diseases and protection 107 (2), 113-123. African Crop Science Conference. : University of Nairobi Case, in the proceedings of the IST-Africa 2008 Conference; Windhoek, Namibia Abstract
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1997

W., PROFMUTITUEUNICE.  1997.  Biological control of root rot of beans caused by Fusarium Oxysporum f.sp. Phaseoli using an antagonist . African Crop Science Conference. : University of Nairobi Case, in the proceedings of the IST-Africa 2008 Conference; Windhoek, Namibia Abstract
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1996

W., PROFMUTITUEUNICE.  1996.  Storage rots. Regional Training course on diagnostic Techniques in plant pathology. African Crop Science Conference. : University of Nairobi Case, in the proceedings of the IST-Africa 2008 Conference; Windhoek, Namibia Abstract
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1994

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