Bio

PROF. MUKUNYA D. MUNGE BIOGRAPHY

Personal Information

PROFESSOR DANIEL MUNGE MUKUNYA, PhD, MKNAS,OGW

ABRIDGED CURRICULUM VITAE

Name of Institution               : University of Nairobi

Publications

2007

  2007.  Characterization of antibiotic meta bolites from actinomycete isolates.

1996

MUNGE, PROFMUKUNYAD.  1996.  Mibey, R.K., K.K. Kokwaro and D.M. Mukunya, 1996. A new species and four new records of Asterina from Kenya. Nova Hedwigia 62: 144 . International Journal at Biochephysics vol.10, 30-33 (2000).. : Plant Molecular Biology Reporter Vol. 27, pp. 79-85. Abstract
The gene Q13L coding for the Capripoxvirus group specific structural protein P32 was expressed in Escherichia coli using plasmid pGEX-2T as a fusion protein with glutathione-s-transferase and purified on glutathione sepharose affinity chromatography column. The protein was then employed for diagnosis of sheeppox, goatpox and lumpyskin disease, by a latex agglutination test (LAT) using the purified P32 antigen and guinea pig detector antiserum raised against the P32 antigen. The LAT and virus neutralization test (VNT) were used to screen one hundred livestock field sera for antibodies to Capripoxvirus, in comparison the LAT was simpler, rapid and 23% more sensitive than the VNT. In addition the LAT was found to be specific for Carpripoxvirus because it did not pick antibodies to Orthopoxvirus and Parapoxvirus. The LA test can be taken for a simple and quick diagnostic tool for primary screening of Carpripoxvirus infection and will reduce the reliance of diagnostic laboratories on tissue culture facilities. Keywords: Carpripox, latex agglutination test, attachment gene J. Trop. Microbiol. Biotechnol. Vol. 3 (2) 2007: pp. 36-43

1992

Mwang'ombe, AW;, Mukunya DM;, Gathuru EM.  1992.  Some Mechanisms Implicated In The Survival Of Benomyl Tolerant Strains Of Colletotrichum Coffeanum Noack, Casual Agent Of Coffee Berry Disease..

1990

Buruchara, RA;, Mukunya DM;, Gathuru EM.  1990.  Bacterial black spot of mangoes in Kenya.
MUNGE, PROFMUKUNYAD.  1990.  Mutitu, E.W., D.M. Mukunya and S.O. Keya, 1990. Effect of moisture on Fusarium yellows of beans caused by Fusarium oxysporum f.sp. phaseoli in Kenya. Accepted for publication in the Kenya National Academy Journal Series B. for 1990.. International Journal at Biochephysics vol.10, 30-33 (2000).. : Plant Molecular Biology Reporter Vol. 27, pp. 79-85. Abstract
The gene Q13L coding for the Capripoxvirus group specific structural protein P32 was expressed in Escherichia coli using plasmid pGEX-2T as a fusion protein with glutathione-s-transferase and purified on glutathione sepharose affinity chromatography column. The protein was then employed for diagnosis of sheeppox, goatpox and lumpyskin disease, by a latex agglutination test (LAT) using the purified P32 antigen and guinea pig detector antiserum raised against the P32 antigen. The LAT and virus neutralization test (VNT) were used to screen one hundred livestock field sera for antibodies to Capripoxvirus, in comparison the LAT was simpler, rapid and 23% more sensitive than the VNT. In addition the LAT was found to be specific for Carpripoxvirus because it did not pick antibodies to Orthopoxvirus and Parapoxvirus. The LA test can be taken for a simple and quick diagnostic tool for primary screening of Carpripoxvirus infection and will reduce the reliance of diagnostic laboratories on tissue culture facilities. Keywords: Carpripox, latex agglutination test, attachment gene J. Trop. Microbiol. Biotechnol. Vol. 3 (2) 2007: pp. 36-43

1989

Mwang'ombe, AW;, Mukunya DM;, Gathuru EM.  1989.  Possible survival mechanisms and control of coffee berry disease fungus strains resistant to Benzimidazole fungicides.
MUNGE, PROFMUKUNYAD.  1989.  Arama, F.P., D. M. Mukunya and R.A. Buruchara, 1989. The influence of temperature on infection of Rhynchosprium sequels on susceptible and resistant Kenya barley varieties.. Proceedings of 1st Plant Pathology Society of Kenya Conference, 23-24 Nov. 1989. Nairobi, Kenya. 15P.. : Plant Molecular Biology Reporter Vol. 27, pp. 79-85. Abstract
The gene Q13L coding for the Capripoxvirus group specific structural protein P32 was expressed in Escherichia coli using plasmid pGEX-2T as a fusion protein with glutathione-s-transferase and purified on glutathione sepharose affinity chromatography column. The protein was then employed for diagnosis of sheeppox, goatpox and lumpyskin disease, by a latex agglutination test (LAT) using the purified P32 antigen and guinea pig detector antiserum raised against the P32 antigen. The LAT and virus neutralization test (VNT) were used to screen one hundred livestock field sera for antibodies to Capripoxvirus, in comparison the LAT was simpler, rapid and 23% more sensitive than the VNT. In addition the LAT was found to be specific for Carpripoxvirus because it did not pick antibodies to Orthopoxvirus and Parapoxvirus. The LA test can be taken for a simple and quick diagnostic tool for primary screening of Carpripoxvirus infection and will reduce the reliance of diagnostic laboratories on tissue culture facilities. Keywords: Carpripox, latex agglutination test, attachment gene J. Trop. Microbiol. Biotechnol. Vol. 3 (2) 2007: pp. 36-43
MUNGE, PROFMUKUNYAD.  1989.  Njiru, J. and D.M. Mukunya, 1989. Variation in Collectotrichum coffeanum, the cause of coffee berry disease as shown by eletrophoretic separation of extracellular proteins of different isolates. Proceedings of the 1st Plant Pathology Society of Kenya Conf. Proceedings of the 1st Plant Pathology Society of Kenya Conference, 23 . : Plant Molecular Biology Reporter Vol. 27, pp. 79-85. Abstract
The gene Q13L coding for the Capripoxvirus group specific structural protein P32 was expressed in Escherichia coli using plasmid pGEX-2T as a fusion protein with glutathione-s-transferase and purified on glutathione sepharose affinity chromatography column. The protein was then employed for diagnosis of sheeppox, goatpox and lumpyskin disease, by a latex agglutination test (LAT) using the purified P32 antigen and guinea pig detector antiserum raised against the P32 antigen. The LAT and virus neutralization test (VNT) were used to screen one hundred livestock field sera for antibodies to Capripoxvirus, in comparison the LAT was simpler, rapid and 23% more sensitive than the VNT. In addition the LAT was found to be specific for Carpripoxvirus because it did not pick antibodies to Orthopoxvirus and Parapoxvirus. The LA test can be taken for a simple and quick diagnostic tool for primary screening of Carpripoxvirus infection and will reduce the reliance of diagnostic laboratories on tissue culture facilities. Keywords: Carpripox, latex agglutination test, attachment gene J. Trop. Microbiol. Biotechnol. Vol. 3 (2) 2007: pp. 36-43
MUNGE, PROFMUKUNYAD.  1989.  Mukunya, D.M. 1989. Plant health for increased crop production and a healthy nation. A keynote address. The 1st Plant Pathology Society of Kenya Conference Proceedings, 23 . International Journal at Biochephysics vol.10, 30-33 (2000).. : Plant Molecular Biology Reporter Vol. 27, pp. 79-85. Abstract
The gene Q13L coding for the Capripoxvirus group specific structural protein P32 was expressed in Escherichia coli using plasmid pGEX-2T as a fusion protein with glutathione-s-transferase and purified on glutathione sepharose affinity chromatography column. The protein was then employed for diagnosis of sheeppox, goatpox and lumpyskin disease, by a latex agglutination test (LAT) using the purified P32 antigen and guinea pig detector antiserum raised against the P32 antigen. The LAT and virus neutralization test (VNT) were used to screen one hundred livestock field sera for antibodies to Capripoxvirus, in comparison the LAT was simpler, rapid and 23% more sensitive than the VNT. In addition the LAT was found to be specific for Carpripoxvirus because it did not pick antibodies to Orthopoxvirus and Parapoxvirus. The LA test can be taken for a simple and quick diagnostic tool for primary screening of Carpripoxvirus infection and will reduce the reliance of diagnostic laboratories on tissue culture facilities. Keywords: Carpripox, latex agglutination test, attachment gene J. Trop. Microbiol. Biotechnol. Vol. 3 (2) 2007: pp. 36-43
MUNGE, PROFMUKUNYAD.  1989.  Kihurani, A.W., D.M. Mukunya and R.A. Burucahra, 1989. Evaluation of bean cultivars for resistance to rust.. Proceedings of the 1st Plant Pathology Society of Kenya Conference, 23 . : Plant Molecular Biology Reporter Vol. 27, pp. 79-85. Abstract
The gene Q13L coding for the Capripoxvirus group specific structural protein P32 was expressed in Escherichia coli using plasmid pGEX-2T as a fusion protein with glutathione-s-transferase and purified on glutathione sepharose affinity chromatography column. The protein was then employed for diagnosis of sheeppox, goatpox and lumpyskin disease, by a latex agglutination test (LAT) using the purified P32 antigen and guinea pig detector antiserum raised against the P32 antigen. The LAT and virus neutralization test (VNT) were used to screen one hundred livestock field sera for antibodies to Capripoxvirus, in comparison the LAT was simpler, rapid and 23% more sensitive than the VNT. In addition the LAT was found to be specific for Carpripoxvirus because it did not pick antibodies to Orthopoxvirus and Parapoxvirus. The LA test can be taken for a simple and quick diagnostic tool for primary screening of Carpripoxvirus infection and will reduce the reliance of diagnostic laboratories on tissue culture facilities. Keywords: Carpripox, latex agglutination test, attachment gene J. Trop. Microbiol. Biotechnol. Vol. 3 (2) 2007: pp. 36-43
MUNGE, PROFMUKUNYAD.  1989.  Mutitu, E.W., D.M. Mukunya and E.M. Gathuru, 1989. Effect of organic amendments on Fusarium yellow disease on the bean host. Discovery and Innovation Vol 1: 67 . International Journal at Biochephysics vol.10, 30-33 (2000).. : Plant Molecular Biology Reporter Vol. 27, pp. 79-85. Abstract
The gene Q13L coding for the Capripoxvirus group specific structural protein P32 was expressed in Escherichia coli using plasmid pGEX-2T as a fusion protein with glutathione-s-transferase and purified on glutathione sepharose affinity chromatography column. The protein was then employed for diagnosis of sheeppox, goatpox and lumpyskin disease, by a latex agglutination test (LAT) using the purified P32 antigen and guinea pig detector antiserum raised against the P32 antigen. The LAT and virus neutralization test (VNT) were used to screen one hundred livestock field sera for antibodies to Capripoxvirus, in comparison the LAT was simpler, rapid and 23% more sensitive than the VNT. In addition the LAT was found to be specific for Carpripoxvirus because it did not pick antibodies to Orthopoxvirus and Parapoxvirus. The LA test can be taken for a simple and quick diagnostic tool for primary screening of Carpripoxvirus infection and will reduce the reliance of diagnostic laboratories on tissue culture facilities. Keywords: Carpripox, latex agglutination test, attachment gene J. Trop. Microbiol. Biotechnol. Vol. 3 (2) 2007: pp. 36-43

1988

Mukunya, DM, Gathuru EM, Omunyin ME.  1988.  Reactions of cultivars of bean (Phaseolus vulgaris L.) to bean common mosaic virus (BCMV). Abstract

3 plants of each cultivar were raised in pots filled with sterile soil, 451 bean cultivars were tested in all. Inoculum was prepared from 30-day-old plants of P. vulgaris. Seedlings of test varieties were inoculated when 2 weeks old, the inoculum consisted of 14 virus isolates ground in 0.01 m phosphate buffer, pH 7.3 and manually inoculated onto primary leaves of seedlings dusted with 500 mesh carborundum. A cultivar was rated resistant if no systemic infection could be detected and susceptible if it became systemically infected or if the presence of the virus in inoculated leaves of plants without systemic symptoms was demonstrated by virus indexing. Only 68 showed some resistance. The other 383 were found susceptible, with 77 of them expressing systemic necrosis, the remainder showing mosaic symptoms
MUNGE, PROFMUKUNYAD.  1988.  Mutitu, E.W., D.M. Mukunya and S.O. Keya, 1988. Effect of antagonistic bacteria isolated from soil organic amendments on Fusarium oxyysporum Schl. In culture. Abstract 5th International congress on Plant Pathology, Kyoto, Japan.. Proceedings of the 1st Symposium of the Crop Science Society of Kenya held on 4-8th July 1988. Nairobi, Kenya. 15 p.. : Plant Molecular Biology Reporter Vol. 27, pp. 79-85. Abstract
The gene Q13L coding for the Capripoxvirus group specific structural protein P32 was expressed in Escherichia coli using plasmid pGEX-2T as a fusion protein with glutathione-s-transferase and purified on glutathione sepharose affinity chromatography column. The protein was then employed for diagnosis of sheeppox, goatpox and lumpyskin disease, by a latex agglutination test (LAT) using the purified P32 antigen and guinea pig detector antiserum raised against the P32 antigen. The LAT and virus neutralization test (VNT) were used to screen one hundred livestock field sera for antibodies to Capripoxvirus, in comparison the LAT was simpler, rapid and 23% more sensitive than the VNT. In addition the LAT was found to be specific for Carpripoxvirus because it did not pick antibodies to Orthopoxvirus and Parapoxvirus. The LA test can be taken for a simple and quick diagnostic tool for primary screening of Carpripoxvirus infection and will reduce the reliance of diagnostic laboratories on tissue culture facilities. Keywords: Carpripox, latex agglutination test, attachment gene J. Trop. Microbiol. Biotechnol. Vol. 3 (2) 2007: pp. 36-43
MUNGE, PROFMUKUNYAD.  1988.  Mwang. Proceedings of the 1st Symposium of the Crop Science Society of Kenya held on 4-8th July 1988. Nairobi, Kenya. 15 p.. : Plant Molecular Biology Reporter Vol. 27, pp. 79-85. Abstract
The gene Q13L coding for the Capripoxvirus group specific structural protein P32 was expressed in Escherichia coli using plasmid pGEX-2T as a fusion protein with glutathione-s-transferase and purified on glutathione sepharose affinity chromatography column. The protein was then employed for diagnosis of sheeppox, goatpox and lumpyskin disease, by a latex agglutination test (LAT) using the purified P32 antigen and guinea pig detector antiserum raised against the P32 antigen. The LAT and virus neutralization test (VNT) were used to screen one hundred livestock field sera for antibodies to Capripoxvirus, in comparison the LAT was simpler, rapid and 23% more sensitive than the VNT. In addition the LAT was found to be specific for Carpripoxvirus because it did not pick antibodies to Orthopoxvirus and Parapoxvirus. The LA test can be taken for a simple and quick diagnostic tool for primary screening of Carpripoxvirus infection and will reduce the reliance of diagnostic laboratories on tissue culture facilities. Keywords: Carpripox, latex agglutination test, attachment gene J. Trop. Microbiol. Biotechnol. Vol. 3 (2) 2007: pp. 36-43
MUNGE, PROFMUKUNYAD.  1988.  Kedera, C.J., D.M. Mukunya and S.O. Keya, 1988. Survival of Rhizobium leguminosarum bv. Phaseoli in contact with Captan.. Proceedings of the 1st Symposium of the Crop Science Society of Kenya held on 4-8th July 1988. Nairobi, Kenya. 15 p.. : Plant Molecular Biology Reporter Vol. 27, pp. 79-85. Abstract
The gene Q13L coding for the Capripoxvirus group specific structural protein P32 was expressed in Escherichia coli using plasmid pGEX-2T as a fusion protein with glutathione-s-transferase and purified on glutathione sepharose affinity chromatography column. The protein was then employed for diagnosis of sheeppox, goatpox and lumpyskin disease, by a latex agglutination test (LAT) using the purified P32 antigen and guinea pig detector antiserum raised against the P32 antigen. The LAT and virus neutralization test (VNT) were used to screen one hundred livestock field sera for antibodies to Capripoxvirus, in comparison the LAT was simpler, rapid and 23% more sensitive than the VNT. In addition the LAT was found to be specific for Carpripoxvirus because it did not pick antibodies to Orthopoxvirus and Parapoxvirus. The LA test can be taken for a simple and quick diagnostic tool for primary screening of Carpripoxvirus infection and will reduce the reliance of diagnostic laboratories on tissue culture facilities. Keywords: Carpripox, latex agglutination test, attachment gene J. Trop. Microbiol. Biotechnol. Vol. 3 (2) 2007: pp. 36-43
MUNGE, PROFMUKUNYAD.  1988.  Mukunya, D.M. 1988. Safe and Effective use of pesticides. Paper presented at the PCAK/GIFP workshop on responsible and effective Crop Protection held in Nairobi, Kenya. 11 . Proceedings of 1st Plant Pathology Society of Kenya Conference, 23-24 Nov. 1989. Nairobi, Kenya. 15P.. : Plant Molecular Biology Reporter Vol. 27, pp. 79-85. Abstract
The gene Q13L coding for the Capripoxvirus group specific structural protein P32 was expressed in Escherichia coli using plasmid pGEX-2T as a fusion protein with glutathione-s-transferase and purified on glutathione sepharose affinity chromatography column. The protein was then employed for diagnosis of sheeppox, goatpox and lumpyskin disease, by a latex agglutination test (LAT) using the purified P32 antigen and guinea pig detector antiserum raised against the P32 antigen. The LAT and virus neutralization test (VNT) were used to screen one hundred livestock field sera for antibodies to Capripoxvirus, in comparison the LAT was simpler, rapid and 23% more sensitive than the VNT. In addition the LAT was found to be specific for Carpripoxvirus because it did not pick antibodies to Orthopoxvirus and Parapoxvirus. The LA test can be taken for a simple and quick diagnostic tool for primary screening of Carpripoxvirus infection and will reduce the reliance of diagnostic laboratories on tissue culture facilities. Keywords: Carpripox, latex agglutination test, attachment gene J. Trop. Microbiol. Biotechnol. Vol. 3 (2) 2007: pp. 36-43
MUNGE, PROFMUKUNYAD.  1988.  Mukunya, D.M. and C. Muinamia, 1988. Rational use of pesticides in Horticulture with special emphasis on Kenya. ACTA HORT. 218: 261 . Proceedings of 1st Plant Pathology Society of Kenya Conference, 23-24 Nov. 1989. Nairobi, Kenya. 15P.. : Plant Molecular Biology Reporter Vol. 27, pp. 79-85. Abstract
The gene Q13L coding for the Capripoxvirus group specific structural protein P32 was expressed in Escherichia coli using plasmid pGEX-2T as a fusion protein with glutathione-s-transferase and purified on glutathione sepharose affinity chromatography column. The protein was then employed for diagnosis of sheeppox, goatpox and lumpyskin disease, by a latex agglutination test (LAT) using the purified P32 antigen and guinea pig detector antiserum raised against the P32 antigen. The LAT and virus neutralization test (VNT) were used to screen one hundred livestock field sera for antibodies to Capripoxvirus, in comparison the LAT was simpler, rapid and 23% more sensitive than the VNT. In addition the LAT was found to be specific for Carpripoxvirus because it did not pick antibodies to Orthopoxvirus and Parapoxvirus. The LA test can be taken for a simple and quick diagnostic tool for primary screening of Carpripoxvirus infection and will reduce the reliance of diagnostic laboratories on tissue culture facilities. Keywords: Carpripox, latex agglutination test, attachment gene J. Trop. Microbiol. Biotechnol. Vol. 3 (2) 2007: pp. 36-43
MUNGE, PROFMUKUNYAD.  1988.  Njeru, R.W., D.M. Mukunya and E.M. Gathuru, 1988. Etiology and chemical control of Cercospora leaf spot of the ornamental bellies of Ireland (Molucella leavis L.) Proceedings of the 1st Symposium of the Crop Science Society of Kenya held on 4 . Proceedings of 1st Plant Pathology Society of Kenya Conference, 23-24 Nov. 1989. Nairobi, Kenya. 15P.. : Plant Molecular Biology Reporter Vol. 27, pp. 79-85. Abstract
The gene Q13L coding for the Capripoxvirus group specific structural protein P32 was expressed in Escherichia coli using plasmid pGEX-2T as a fusion protein with glutathione-s-transferase and purified on glutathione sepharose affinity chromatography column. The protein was then employed for diagnosis of sheeppox, goatpox and lumpyskin disease, by a latex agglutination test (LAT) using the purified P32 antigen and guinea pig detector antiserum raised against the P32 antigen. The LAT and virus neutralization test (VNT) were used to screen one hundred livestock field sera for antibodies to Capripoxvirus, in comparison the LAT was simpler, rapid and 23% more sensitive than the VNT. In addition the LAT was found to be specific for Carpripoxvirus because it did not pick antibodies to Orthopoxvirus and Parapoxvirus. The LA test can be taken for a simple and quick diagnostic tool for primary screening of Carpripoxvirus infection and will reduce the reliance of diagnostic laboratories on tissue culture facilities. Keywords: Carpripox, latex agglutination test, attachment gene J. Trop. Microbiol. Biotechnol. Vol. 3 (2) 2007: pp. 36-43

1987

MUNGE, PROFMUKUNYAD.  1987.  Omunyin, M.E., E.M. Gathuru and D.M. Mukunya, 1987. Reactions of cultivars of beans (Phaseolus vulgaris L.) to bean common mosaic virus (BCMV). Trop. Agric. (Trinidad) 65 (2): 166 . Proceedings of the 1st Symposium of the Crop Science Society of Kenya held on 4-8th July 1988. Nairobi, Kenya. 15 p.. : Plant Molecular Biology Reporter Vol. 27, pp. 79-85. Abstract
The gene Q13L coding for the Capripoxvirus group specific structural protein P32 was expressed in Escherichia coli using plasmid pGEX-2T as a fusion protein with glutathione-s-transferase and purified on glutathione sepharose affinity chromatography column. The protein was then employed for diagnosis of sheeppox, goatpox and lumpyskin disease, by a latex agglutination test (LAT) using the purified P32 antigen and guinea pig detector antiserum raised against the P32 antigen. The LAT and virus neutralization test (VNT) were used to screen one hundred livestock field sera for antibodies to Capripoxvirus, in comparison the LAT was simpler, rapid and 23% more sensitive than the VNT. In addition the LAT was found to be specific for Carpripoxvirus because it did not pick antibodies to Orthopoxvirus and Parapoxvirus. The LA test can be taken for a simple and quick diagnostic tool for primary screening of Carpripoxvirus infection and will reduce the reliance of diagnostic laboratories on tissue culture facilities. Keywords: Carpripox, latex agglutination test, attachment gene J. Trop. Microbiol. Biotechnol. Vol. 3 (2) 2007: pp. 36-43
MUNGE, PROFMUKUNYAD.  1987.  Mukunya D.M. 1987. Pesticides in our environment. Presented at the Regional Workshop on Environmental Protection for the Greenbelt Movement held at the college of Education and External Studies, Kikuyu, University of Nairobi, Kenya. 11p. Proceedings of the 1st Symposium of the Crop Science Society of Kenya held on 4-8th July 1988. Nairobi, Kenya. 15 p.. : Plant Molecular Biology Reporter Vol. 27, pp. 79-85. Abstract
The gene Q13L coding for the Capripoxvirus group specific structural protein P32 was expressed in Escherichia coli using plasmid pGEX-2T as a fusion protein with glutathione-s-transferase and purified on glutathione sepharose affinity chromatography column. The protein was then employed for diagnosis of sheeppox, goatpox and lumpyskin disease, by a latex agglutination test (LAT) using the purified P32 antigen and guinea pig detector antiserum raised against the P32 antigen. The LAT and virus neutralization test (VNT) were used to screen one hundred livestock field sera for antibodies to Capripoxvirus, in comparison the LAT was simpler, rapid and 23% more sensitive than the VNT. In addition the LAT was found to be specific for Carpripoxvirus because it did not pick antibodies to Orthopoxvirus and Parapoxvirus. The LA test can be taken for a simple and quick diagnostic tool for primary screening of Carpripoxvirus infection and will reduce the reliance of diagnostic laboratories on tissue culture facilities. Keywords: Carpripox, latex agglutination test, attachment gene J. Trop. Microbiol. Biotechnol. Vol. 3 (2) 2007: pp. 36-43
MUNGE, PROFMUKUNYAD.  1987.  Mukunya D.M. 1987. Pesticides in our environment. Presented at the Regional Workshop on Environmental Protection for the Greenbelt Movement held at the college of Education and External Studies, Kikuyu, University of Nairobi, Kenya. 11p. Proceedings of the 1st Symposium of the Crop Science Society of Kenya held on 4-8th July 1988. Nairobi, Kenya. 15 p.. : Plant Molecular Biology Reporter Vol. 27, pp. 79-85. Abstract
The gene Q13L coding for the Capripoxvirus group specific structural protein P32 was expressed in Escherichia coli using plasmid pGEX-2T as a fusion protein with glutathione-s-transferase and purified on glutathione sepharose affinity chromatography column. The protein was then employed for diagnosis of sheeppox, goatpox and lumpyskin disease, by a latex agglutination test (LAT) using the purified P32 antigen and guinea pig detector antiserum raised against the P32 antigen. The LAT and virus neutralization test (VNT) were used to screen one hundred livestock field sera for antibodies to Capripoxvirus, in comparison the LAT was simpler, rapid and 23% more sensitive than the VNT. In addition the LAT was found to be specific for Carpripoxvirus because it did not pick antibodies to Orthopoxvirus and Parapoxvirus. The LA test can be taken for a simple and quick diagnostic tool for primary screening of Carpripoxvirus infection and will reduce the reliance of diagnostic laboratories on tissue culture facilities. Keywords: Carpripox, latex agglutination test, attachment gene J. Trop. Microbiol. Biotechnol. Vol. 3 (2) 2007: pp. 36-43
MUNGE, PROFMUKUNYAD.  1987.  Gathuru, E.M. and D.M. Mukunya and R.A. Buruchara, 1987. Diseases of crop plants in small scale farms of Embu Agro-ecozone I-IV and farmers awareness on disease control methods. In integrated Crop Protection for Small Scale Farmers in Eastern Africa. Comm. Proceedings of the 1st Symposium of the Crop Science Society of Kenya held on 4-8th July 1988. Nairobi, Kenya. 15 p.. : Plant Molecular Biology Reporter Vol. 27, pp. 79-85. Abstract
The gene Q13L coding for the Capripoxvirus group specific structural protein P32 was expressed in Escherichia coli using plasmid pGEX-2T as a fusion protein with glutathione-s-transferase and purified on glutathione sepharose affinity chromatography column. The protein was then employed for diagnosis of sheeppox, goatpox and lumpyskin disease, by a latex agglutination test (LAT) using the purified P32 antigen and guinea pig detector antiserum raised against the P32 antigen. The LAT and virus neutralization test (VNT) were used to screen one hundred livestock field sera for antibodies to Capripoxvirus, in comparison the LAT was simpler, rapid and 23% more sensitive than the VNT. In addition the LAT was found to be specific for Carpripoxvirus because it did not pick antibodies to Orthopoxvirus and Parapoxvirus. The LA test can be taken for a simple and quick diagnostic tool for primary screening of Carpripoxvirus infection and will reduce the reliance of diagnostic laboratories on tissue culture facilities. Keywords: Carpripox, latex agglutination test, attachment gene J. Trop. Microbiol. Biotechnol. Vol. 3 (2) 2007: pp. 36-43
MUNGE, PROFMUKUNYAD.  1987.  Kimani, V.W. and D.M. Mukunya, 1987. Control of pesticides residues in food. Paper presented at the National Food Control Seminar, Kenya Bureau of Standards. 6-9th Oct. 1987. Nairobi, Kenya.. Proceedings of the 1st Symposium of the Crop Science Society of Kenya held on 4-8th July 1988. Nairobi, Kenya. 15 p.. : Plant Molecular Biology Reporter Vol. 27, pp. 79-85. Abstract
The gene Q13L coding for the Capripoxvirus group specific structural protein P32 was expressed in Escherichia coli using plasmid pGEX-2T as a fusion protein with glutathione-s-transferase and purified on glutathione sepharose affinity chromatography column. The protein was then employed for diagnosis of sheeppox, goatpox and lumpyskin disease, by a latex agglutination test (LAT) using the purified P32 antigen and guinea pig detector antiserum raised against the P32 antigen. The LAT and virus neutralization test (VNT) were used to screen one hundred livestock field sera for antibodies to Capripoxvirus, in comparison the LAT was simpler, rapid and 23% more sensitive than the VNT. In addition the LAT was found to be specific for Carpripoxvirus because it did not pick antibodies to Orthopoxvirus and Parapoxvirus. The LA test can be taken for a simple and quick diagnostic tool for primary screening of Carpripoxvirus infection and will reduce the reliance of diagnostic laboratories on tissue culture facilities. Keywords: Carpripox, latex agglutination test, attachment gene J. Trop. Microbiol. Biotechnol. Vol. 3 (2) 2007: pp. 36-43

1986

Mukunya, DM, Gatharu EM, Omunyin ME.  1986.  Strains of BCMV and their interaction with I-gene bean varieties.
MUNGE, PROFMUKUNYAD.  1986.  Omunyin, M.E. Gathuru and D.M. Mukunya, 1986. Las Cepas, del BCMCY. Su. Interaction Con Variedades de Frijol Con el Gene 1. Hojas de Frijol 8 (3).. Proceedings of the 1st Symposium of the Crop Science Society of Kenya held on 4-8th July 1988. Nairobi, Kenya. 15 p.. : Plant Molecular Biology Reporter Vol. 27, pp. 79-85. Abstract
The gene Q13L coding for the Capripoxvirus group specific structural protein P32 was expressed in Escherichia coli using plasmid pGEX-2T as a fusion protein with glutathione-s-transferase and purified on glutathione sepharose affinity chromatography column. The protein was then employed for diagnosis of sheeppox, goatpox and lumpyskin disease, by a latex agglutination test (LAT) using the purified P32 antigen and guinea pig detector antiserum raised against the P32 antigen. The LAT and virus neutralization test (VNT) were used to screen one hundred livestock field sera for antibodies to Capripoxvirus, in comparison the LAT was simpler, rapid and 23% more sensitive than the VNT. In addition the LAT was found to be specific for Carpripoxvirus because it did not pick antibodies to Orthopoxvirus and Parapoxvirus. The LA test can be taken for a simple and quick diagnostic tool for primary screening of Carpripoxvirus infection and will reduce the reliance of diagnostic laboratories on tissue culture facilities. Keywords: Carpripox, latex agglutination test, attachment gene J. Trop. Microbiol. Biotechnol. Vol. 3 (2) 2007: pp. 36-43
MUNGE, PROFMUKUNYAD.  1986.  Mathangya, P.M., D.M. Mukunya and E.M. Gathuru, 1986. Aetiology and sources of resistance to common blight (Xanthomonas campestris pv. Phaseolus (Smith, 1987). Dye,1978, of beans (phaseolus vulgaris L. ) in Kenya. Kenya Jour. Of Science Series B. 7 (2): 2. Proceedings of the 1st Symposium of the Crop Science Society of Kenya held on 4-8th July 1988. Nairobi, Kenya. 15 p.. : Plant Molecular Biology Reporter Vol. 27, pp. 79-85. Abstract
The gene Q13L coding for the Capripoxvirus group specific structural protein P32 was expressed in Escherichia coli using plasmid pGEX-2T as a fusion protein with glutathione-s-transferase and purified on glutathione sepharose affinity chromatography column. The protein was then employed for diagnosis of sheeppox, goatpox and lumpyskin disease, by a latex agglutination test (LAT) using the purified P32 antigen and guinea pig detector antiserum raised against the P32 antigen. The LAT and virus neutralization test (VNT) were used to screen one hundred livestock field sera for antibodies to Capripoxvirus, in comparison the LAT was simpler, rapid and 23% more sensitive than the VNT. In addition the LAT was found to be specific for Carpripoxvirus because it did not pick antibodies to Orthopoxvirus and Parapoxvirus. The LA test can be taken for a simple and quick diagnostic tool for primary screening of Carpripoxvirus infection and will reduce the reliance of diagnostic laboratories on tissue culture facilities. Keywords: Carpripox, latex agglutination test, attachment gene J. Trop. Microbiol. Biotechnol. Vol. 3 (2) 2007: pp. 36-43
MUNGE, PROFMUKUNYAD.  1986.  Bondole wa Mbula, E.M. Gathuru, 1986. Malakisi tobacco necrosis virus. Proceeding of the symposium on viral diseases in Africa held in Nairobi, Kenya. O.A.U. Scientific and Technical Res. Council Publication.. Proceedings of the 1st Symposium of the Crop Science Society of Kenya held on 4-8th July 1988. Nairobi, Kenya. 15 p.. : Plant Molecular Biology Reporter Vol. 27, pp. 79-85. Abstract
The gene Q13L coding for the Capripoxvirus group specific structural protein P32 was expressed in Escherichia coli using plasmid pGEX-2T as a fusion protein with glutathione-s-transferase and purified on glutathione sepharose affinity chromatography column. The protein was then employed for diagnosis of sheeppox, goatpox and lumpyskin disease, by a latex agglutination test (LAT) using the purified P32 antigen and guinea pig detector antiserum raised against the P32 antigen. The LAT and virus neutralization test (VNT) were used to screen one hundred livestock field sera for antibodies to Capripoxvirus, in comparison the LAT was simpler, rapid and 23% more sensitive than the VNT. In addition the LAT was found to be specific for Carpripoxvirus because it did not pick antibodies to Orthopoxvirus and Parapoxvirus. The LA test can be taken for a simple and quick diagnostic tool for primary screening of Carpripoxvirus infection and will reduce the reliance of diagnostic laboratories on tissue culture facilities. Keywords: Carpripox, latex agglutination test, attachment gene J. Trop. Microbiol. Biotechnol. Vol. 3 (2) 2007: pp. 36-43

1985

MUNGE, PROFMUKUNYAD.  1985.  Mutitu, E.W., D.M. Mukunya and S.O. Keya, 1985, Biological control of Fusarium yellow on beans caused by Fusarium oxysporum Schl. F.sp phaseolus Kendrick and Snyder using organic amendments. ACTA HORT. 218:267 . Proceedings of the 1st Symposium of the Crop Science Society of Kenya held on 4-8th July 1988. Nairobi, Kenya. 15 p.. : Plant Molecular Biology Reporter Vol. 27, pp. 79-85. Abstract
The gene Q13L coding for the Capripoxvirus group specific structural protein P32 was expressed in Escherichia coli using plasmid pGEX-2T as a fusion protein with glutathione-s-transferase and purified on glutathione sepharose affinity chromatography column. The protein was then employed for diagnosis of sheeppox, goatpox and lumpyskin disease, by a latex agglutination test (LAT) using the purified P32 antigen and guinea pig detector antiserum raised against the P32 antigen. The LAT and virus neutralization test (VNT) were used to screen one hundred livestock field sera for antibodies to Capripoxvirus, in comparison the LAT was simpler, rapid and 23% more sensitive than the VNT. In addition the LAT was found to be specific for Carpripoxvirus because it did not pick antibodies to Orthopoxvirus and Parapoxvirus. The LA test can be taken for a simple and quick diagnostic tool for primary screening of Carpripoxvirus infection and will reduce the reliance of diagnostic laboratories on tissue culture facilities. Keywords: Carpripox, latex agglutination test, attachment gene J. Trop. Microbiol. Biotechnol. Vol. 3 (2) 2007: pp. 36-43
MUNGE, PROFMUKUNYAD.  1985.  Buruchara, R.A., E.M.Gathuru and D.M. Mukunya, 1985. Disease progress of angular leaf spot caused by Isariopsis griseola sacc. And it implications on resistance of some bean (Phaseolus vulgaris L.) cultivars. ACTA HORTICULATURAE 218: 321 . Proceedings of the 1st Symposium of the Crop Science Society of Kenya held on 4-8th July 1988. Nairobi, Kenya. 15 p.. : Plant Molecular Biology Reporter Vol. 27, pp. 79-85. Abstract
The gene Q13L coding for the Capripoxvirus group specific structural protein P32 was expressed in Escherichia coli using plasmid pGEX-2T as a fusion protein with glutathione-s-transferase and purified on glutathione sepharose affinity chromatography column. The protein was then employed for diagnosis of sheeppox, goatpox and lumpyskin disease, by a latex agglutination test (LAT) using the purified P32 antigen and guinea pig detector antiserum raised against the P32 antigen. The LAT and virus neutralization test (VNT) were used to screen one hundred livestock field sera for antibodies to Capripoxvirus, in comparison the LAT was simpler, rapid and 23% more sensitive than the VNT. In addition the LAT was found to be specific for Carpripoxvirus because it did not pick antibodies to Orthopoxvirus and Parapoxvirus. The LA test can be taken for a simple and quick diagnostic tool for primary screening of Carpripoxvirus infection and will reduce the reliance of diagnostic laboratories on tissue culture facilities. Keywords: Carpripox, latex agglutination test, attachment gene J. Trop. Microbiol. Biotechnol. Vol. 3 (2) 2007: pp. 36-43
MUNGE, PROFMUKUNYAD.  1985.  Buruchara, R.A., E.M.Gathuru and D.M. Mukunya, 1985. Disease progress of angular leaf spot caused by Isariopsis griseola sacc. And it implications on resistance of some bean (Phaseolus vulgaris L.) cultivars. ACTA HORTICULATURAE 218: 321 . Proceedings of the 1st Symposium of the Crop Science Society of Kenya held on 4-8th July 1988. Nairobi, Kenya. 15 p.. : Plant Molecular Biology Reporter Vol. 27, pp. 79-85. Abstract
The gene Q13L coding for the Capripoxvirus group specific structural protein P32 was expressed in Escherichia coli using plasmid pGEX-2T as a fusion protein with glutathione-s-transferase and purified on glutathione sepharose affinity chromatography column. The protein was then employed for diagnosis of sheeppox, goatpox and lumpyskin disease, by a latex agglutination test (LAT) using the purified P32 antigen and guinea pig detector antiserum raised against the P32 antigen. The LAT and virus neutralization test (VNT) were used to screen one hundred livestock field sera for antibodies to Capripoxvirus, in comparison the LAT was simpler, rapid and 23% more sensitive than the VNT. In addition the LAT was found to be specific for Carpripoxvirus because it did not pick antibodies to Orthopoxvirus and Parapoxvirus. The LA test can be taken for a simple and quick diagnostic tool for primary screening of Carpripoxvirus infection and will reduce the reliance of diagnostic laboratories on tissue culture facilities. Keywords: Carpripox, latex agglutination test, attachment gene J. Trop. Microbiol. Biotechnol. Vol. 3 (2) 2007: pp. 36-43

1984

MUNGE, PROFMUKUNYAD.  1984.  Nyangeri, J.P., E.M. Gathuru and D.M. Mukunya, 1984. Effect of latent infection on the spread of Bacterial wilt of Potatoes in Kenya. Trop. Pest management 30 (2): 163 . Proceedings of the 1st Symposium of the Crop Science Society of Kenya held on 4-8th July 1988. Nairobi, Kenya. 15 p.. : Plant Molecular Biology Reporter Vol. 27, pp. 79-85. Abstract
The gene Q13L coding for the Capripoxvirus group specific structural protein P32 was expressed in Escherichia coli using plasmid pGEX-2T as a fusion protein with glutathione-s-transferase and purified on glutathione sepharose affinity chromatography column. The protein was then employed for diagnosis of sheeppox, goatpox and lumpyskin disease, by a latex agglutination test (LAT) using the purified P32 antigen and guinea pig detector antiserum raised against the P32 antigen. The LAT and virus neutralization test (VNT) were used to screen one hundred livestock field sera for antibodies to Capripoxvirus, in comparison the LAT was simpler, rapid and 23% more sensitive than the VNT. In addition the LAT was found to be specific for Carpripoxvirus because it did not pick antibodies to Orthopoxvirus and Parapoxvirus. The LA test can be taken for a simple and quick diagnostic tool for primary screening of Carpripoxvirus infection and will reduce the reliance of diagnostic laboratories on tissue culture facilities. Keywords: Carpripox, latex agglutination test, attachment gene J. Trop. Microbiol. Biotechnol. Vol. 3 (2) 2007: pp. 36-43

1983

Muigai, SGS;, Njuguna SK;, Mukunya DM;, Ngugi DN.  1983.  Current bean research programmes in Kenya..

1982

Mukunya, DM.  1982.  Undp/fao Evaluation Study On National Agricultural Research, Kenya 195 +35 Annex Tables, Fao, Rome..
Kinyua, GK;, Mukunya DM;, Van Breukelen EM.  1982.  Studies on genetic resistance of beans to Pseudomonas phaseolicola in Kenya.
Omunyin, ME, Gathuru;, Mukunya DM.  1982.  Las Cepas, del BCMCY. Su. Interaction Con Variedades de Frijol Con el Gene 1.
Mukunya, DM;, Gichuru EM;, Itulya FM;, Coulson CL.  1982.  Improvement of beans in the semi-arid areas of Kenya: Report prepared for the University of Nairobi and Bean/Cowpea CRSP.
Onyango, D;, Mukunya DM.  1982.  Bacterial Blight Of Cassava, Xanthomonas Manihotis In Kenya..
MUNGE, PROFMUKUNYAD.  1982.  Mukunya, D.M. 1982. UNDP/FAO Evaluation study on National Agricultural Research, Kenya 195 +35 Annex tables, FAO, ROME.. Proceedings of the Workshop on root and Tuber crops held in Burundi. IITA. Ibadan, Nigeria.. : Plant Molecular Biology Reporter Vol. 27, pp. 79-85. Abstract
The gene Q13L coding for the Capripoxvirus group specific structural protein P32 was expressed in Escherichia coli using plasmid pGEX-2T as a fusion protein with glutathione-s-transferase and purified on glutathione sepharose affinity chromatography column. The protein was then employed for diagnosis of sheeppox, goatpox and lumpyskin disease, by a latex agglutination test (LAT) using the purified P32 antigen and guinea pig detector antiserum raised against the P32 antigen. The LAT and virus neutralization test (VNT) were used to screen one hundred livestock field sera for antibodies to Capripoxvirus, in comparison the LAT was simpler, rapid and 23% more sensitive than the VNT. In addition the LAT was found to be specific for Carpripoxvirus because it did not pick antibodies to Orthopoxvirus and Parapoxvirus. The LA test can be taken for a simple and quick diagnostic tool for primary screening of Carpripoxvirus infection and will reduce the reliance of diagnostic laboratories on tissue culture facilities. Keywords: Carpripox, latex agglutination test, attachment gene J. Trop. Microbiol. Biotechnol. Vol. 3 (2) 2007: pp. 36-43
MUNGE, PROFMUKUNYAD.  1982.  Mukunya, D.M. 1982. Development of crop varieties for disease resistance.. Proceedings of the third FAO/SIDA seminar on . : Plant Molecular Biology Reporter Vol. 27, pp. 79-85. Abstract
The gene Q13L coding for the Capripoxvirus group specific structural protein P32 was expressed in Escherichia coli using plasmid pGEX-2T as a fusion protein with glutathione-s-transferase and purified on glutathione sepharose affinity chromatography column. The protein was then employed for diagnosis of sheeppox, goatpox and lumpyskin disease, by a latex agglutination test (LAT) using the purified P32 antigen and guinea pig detector antiserum raised against the P32 antigen. The LAT and virus neutralization test (VNT) were used to screen one hundred livestock field sera for antibodies to Capripoxvirus, in comparison the LAT was simpler, rapid and 23% more sensitive than the VNT. In addition the LAT was found to be specific for Carpripoxvirus because it did not pick antibodies to Orthopoxvirus and Parapoxvirus. The LA test can be taken for a simple and quick diagnostic tool for primary screening of Carpripoxvirus infection and will reduce the reliance of diagnostic laboratories on tissue culture facilities. Keywords: Carpripox, latex agglutination test, attachment gene J. Trop. Microbiol. Biotechnol. Vol. 3 (2) 2007: pp. 36-43
MUNGE, PROFMUKUNYAD.  1982.  Onyango, D. and D.M. Mukunya. 1982. Bacterial blight of cassava, Xanthomonas manihotis in Kenya.. Proceedings of the Workshop on root and Tuber crops held in Burundi. IITA. Ibadan, Nigeria.. : Plant Molecular Biology Reporter Vol. 27, pp. 79-85. Abstract
The gene Q13L coding for the Capripoxvirus group specific structural protein P32 was expressed in Escherichia coli using plasmid pGEX-2T as a fusion protein with glutathione-s-transferase and purified on glutathione sepharose affinity chromatography column. The protein was then employed for diagnosis of sheeppox, goatpox and lumpyskin disease, by a latex agglutination test (LAT) using the purified P32 antigen and guinea pig detector antiserum raised against the P32 antigen. The LAT and virus neutralization test (VNT) were used to screen one hundred livestock field sera for antibodies to Capripoxvirus, in comparison the LAT was simpler, rapid and 23% more sensitive than the VNT. In addition the LAT was found to be specific for Carpripoxvirus because it did not pick antibodies to Orthopoxvirus and Parapoxvirus. The LA test can be taken for a simple and quick diagnostic tool for primary screening of Carpripoxvirus infection and will reduce the reliance of diagnostic laboratories on tissue culture facilities. Keywords: Carpripox, latex agglutination test, attachment gene J. Trop. Microbiol. Biotechnol. Vol. 3 (2) 2007: pp. 36-43

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