Gathuma, J.M. and Kagiko, M.M. (1980). Veterinary science and human health. Proceedings of the 9th Council Meeting of Commonwealth Medical Association and 9th Annual Scientific Conference of Kenya Medical Association (15th-29th March, 1980)

Citation:
MUCHAI PROFKAGIKOM. "Gathuma, J.M. and Kagiko, M.M. (1980). Veterinary science and human health. Proceedings of the 9th Council Meeting of Commonwealth Medical Association and 9th Annual Scientific Conference of Kenya Medical Association (15th-29th March, 1980).". In: journal. FARA; 1980.

Abstract:

Experimentally, two hydatid cyst fluid (HCF) antigens (antigens 4 and 5) were found to be the most immunogenic antigens in HCF.  The two antigens were precipitated together from HCF.  This was done by adding 2M phosphotungstic acid and 2M magnesium chloride pollutions to clarified HCF whilte continuously stirring the mixture.  The precipitate formed was suspend in physiological saline (PS).  This antigens solutions was used to coat microtitre plates fro indirect ELISA.  Indirect ELISA was performed on 180 randomly selected bovine sera.
The sensitivity of the test was found to be 98% while the specificity was 70%.  The predictive value was 89%.  Although the specificity of the test was relatively low, the test using these partially purified antigens was found to be useful because of its high sensitivity.

Notes:

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