WAWERU DRGAKUYADANIEL. "
Gakuya, D.W., Mbithi, P.M.F., Maitho, T.E. and Musimba, N.K.R. 2004. The potential use of plant anthelmintic for the control of livestock helminthoses in Kenya. The Kenya Veterinarian 26: 14-26.". In:
Journal of Etnopharmacology. Elsevier; 2004.
AbstractHelminthoses is a major constraint to livestock production in Kenya. The control of these infections by the use of synthetic anthelmintics is constrained by a number of problems that include; lack of foreign exchange to import them, development of anthelmintic resistance, lack of finance to purchase them, unavailability and misuse due to lack of appropriate information. The use of plant anthelmintics would be a rational alternative to bridge these shortfalls. In some tropical countries, research work has been carried out to validate this possibility. In order to integrate plant anthelmintics in the overall helminth control in Kenya, there is a need to document research findings on indigenous knowledge, through carrying out experiments to determine efficacy, toxicity and optimum dosage rates. In addition, isolation and identification of active ingredients using current laboratory techniques will be a critical milestone.
WAWERU DRGAKUYADANIEL. "
THE POTENTIAL USE OF PLANT ANTHELMINTICS FOR THE CONTROL OF LIVESTOCK HELMINTHOSES IN KENYA.The Kenya Veterinarian Vol. 26, 2004.". In:
Journal of Etnopharmacology. Elsevier; 2004.
AbstractD.W. Gakuya,1 P.M.F. Mbithi 1, T.E. Maitho2 and N.K.R. Musimba3 Department of Clinical Studies, 2. Department of Public Health, Pharmacology and Toxicology 3 Department of Range Management, University of Nairobi. Kenya. Abstract Helminthoses is a major constraint to livestock production in Kenya. The control of these infections by the use of synthetic anthelmintics is constrained by a number of problems that include; lack of foreign exchange to import them, development of anthelmintic resistance, lack of finance to purchase them, unavailability and misuse due to lack of appropriate information. The use of plant anthelmintics would be a rational alternative to bridge these shortfalls. In some tropical countries, research work has been carried out to validate this possibility. In order to integrate plant anthelmintics in the overall helminth control in Kenya, there is a need to document research findings on indigenous knowledge, through carrying out experiments to determine efficacy, toxicity and optimum dosage rates. In addition, isolation and identification of active ingredients using current laboratory techniques will be a critical milestone
WAWERU DRGAKUYADANIEL. "
Gakuya,D.W.,Mbithi,P.M.F.,Mugambi,J.M.,Maitho,T.E.and Musimba,N.K.R.2005. Evaluation of the efficacy of aqueous extracts of Albizia anthelmintica and Maerua edulis against the nematode Heligsomoides polygyrus infections in mice. The Kenya Veterinarian 28:.". In:
Journal of Etnopharmacology. Elsevier; 2005.
AbstractEthnopharmacological relevance: Traditional medicines play an important role in the management of chronically painful and debilitating joint conditions, particularly in the rural Africa. However, their potential use as sources of medicines has not been fully exploited. The present study was carried to find the medicinal plants traditionally used to manage chronic joint pains in Machakos and Makueni counties in Kenya. Materials and methods: To obtain this ethnobotanical information, 30 consenting traditional herbal med-ical practitioners were interviewed exclusively on medicinal plant use in the management of chronic joint pains, in a pre-planned workshop. Results and discussion: In this survey, a total of 37 plants belonging to 32 genera and 23 families were cited as being important for treatment of chronic joint pains. The most commonly cited plant species were Pavetta crassipes K. Schum, Strychnos henningsii Gilg., Carissa spinarum L., Fagaropsis hildebrandtii (Engl.) Milve-Redh. and Zanthoxylum chalybeum Engl. Acacia mellifera (Vahl) Benth., Amaranthus albus L., Balanites glabra Mildbr. & Schltr., Grewia fallax K. Schum., Lactuca capensis, Launaea cornuta (Oliv. & Hiern) O. Jeffrey, Lippia kituiensis Vatke, Pappea capensis Eckl. & Zeyh. and Pennisetum glaucum (L.) R. Br. are documented for the first time as being important in the management of chronic joint pains. Conclusions: The findings of this study show that a variety of medicinal plants are used in the management of chronic joint pains and the main mode of administration is oral. Keywords: Ethnobotanical survey; Medicinal plants; Chronic joint pains; Rheumatoid arthritis; Akamba; Machakos-Kenya
WAWERU DRGAKUYADANIEL. "
The Kenya Veterinarian Vol. 29,2005. PRELIMINARY INVESTIGATION ON THE STATUS OF BOVINE MASTITIS IN A MEDIUM SCALE DAIRY FARM IN KABETE AREA, KENYA.". In:
Journal of Etnopharmacology. Elsevier; 2005.
AbstractThe purpose of this investigation was to establish the mastitis status in the dairy herd of 110 cows (genetically heterogeneous Bos Taurus breeds). All the cows were examined by physical inspection of the udder and California Mastitis Test (CMT) carried out on milk from the quarters of the 89 cows in milk. A total of 37 milk samples were collected from affected quarters of 36 cows, which had visibly abnormal secretion or tested positive on CMT, and taken for bacteriological culture and sensitivity tests. The outcome was a prevalence of 40.4 % based on the cows in milk and 18.4% based on their quarters. From the 37 samples that were cultured, 36 had abundant bacterial growth. Of the bacteria isolated, 45 % were Corynebacteria, 30.0 % Staphylococci, 22.5% Streptococci and 2.65% Pseudomonas. Of the twelve antibacterial compounds used in the sensitivity tests, the bacteria were most sensitive to ampi-clox and cefaclor at 98% and lest sensitive to sulphamethoxazole and cotrimoxazole both at 2%. Pseudomonas was only sensitive to gentamycin, norfloxacin and tetracycline. From this investigation the prevalence of mastitis in the herd is fairly high and there is need for a comprehensive monitoring and control program for udder health in this herd
WAWERU DRGAKUYADANIEL. "
EVALUATION OF THE EFFICACY OF AQUEOUS EXTRACTS OF ALBIZIA ANTHELMINTICA AND MAERUA EDULIS AGAINST THE NEMATODE HELIGMOSOMOIDES POLYGYRUS INFECTIONS IN MICE. The Kenya Veterinarian Vol. 28,2005.". In:
Journal of Etnopharmacology. Elsevier; 2005.
AbstractAnthelmintic activity of the water extracts of Albizia anthelmintica bark and Maerua- edulis root was evaluated in mice that had been experimentally infected with the intestinal nematode Heligmosomoides polygyrus. The mice were randomly allocated into six treatment groups and one control group. Groups, 2, and 3 were given an oral dose of water extracts of A. anthelmintica at 5gm/kg, 10gm/kg and 20gm/kg bodyweight respectively in a divided dose on day 17 post-infection. Groups 4, 5 and 6 were given water extracts of M. edulis at a dosage of 5gm/kg, 10gm/kg and 20gm/kg bodyweight respectively in a divided dose. Group 7 was the control and was concurrently given a double oral dose of 0.2ml of physiological saline each. Mortality of some mice was observed in four groups after treatment. Five days after treatment, faecal worm egg count reduction was determined. The results showed a percentage faecal H. polygyus egg count reduction of 72%. 69%, 50%, 42% in groups 2,6,3 and 1 respectively. Seven days after treatment there was a reduction in worm counts at postmortem of 68%, 36%, /5%, 19%, 16% and 14% in groups 1,5,2 3,6, and 4 respectively compared to untreated controls. These results indicate that the plant extracts had anthelmintic activity and support the use of these plants as anthelmintics
WAWERU DRGAKUYADANIEL. "
Gakuya, D.W., Mbaria, J.M., Mbithi,P.M.F. and Munenge, R.W. 2004. Evaluation of the bioactivity of some traditional medicinal plants using the Brine Shrimp Lethality Test. The Kenya Veterinarian 26: 8-11.". In:
Journal of Etnopharmacology. Elsevier; 2004.
AbstractThe purpose of this experiment was to evaluate the bioactivity of extracts of Chrysanthemum cineraraefolium Vis., Albizia anthelmintica A Brgn, Maerua edulis (Gilg) De wolf and Myrsine Africana L. Which are using tratitioanll as anthelmintic as using brine shrimp lethanitly test serial dilutions of 1000 ug/ml and 10 ug / ml of the extracts were put in five test tubes. Ten (10) brine shrimp larvae were immersed into each of the test tubes and the number surviving after 24 hours counted and the percentage mortality and LC for each extract was determined. Cl cinerariaefolium extract (pyrethrins) was active (LC 1000 ug/ml) of 18ug/ml. The methanol extacts of Maerua edulis, Maera subcordata and Myysine Africana were not active (LC 1000 ug/ml) the results indicated that C. cenerareafolium and A. Anthelmintica extracts have bioactivity and is the basis for their use as anthelmintic by pastoral communities. Brine shrimp lethality test was found to be a simple and rapid test and is thus recommended for similar studies.
WASWA AARONKUTUKHULU. Petrology and iron ore mineralization in the Neoproterozoic Mozambique belt rocks of Mutomo-Ikutha area, in Kitui county, S.E. Kenya. Nyamai DCM, Mathu PE, ICHANG'I DD, eds. Nairobi, Kenya: UNIVERSITY OF NAIROBI; 2015.
AbstractPETROLOGY AND TECTONO - METAMORPHIC SETTING OF IRON DEPOSITS IN THE MOZAMBIQUE BELT SEGMENT IN MUTOMO - IKUTHA AREA, SOUTH- EASTERN KENYA
Abstract
The study area is bounded by longitudes 380 4’E to 38020’E and latitudes 1048’S to 208’S in South Kitui within the Kitui County occupying about 100 Km2. This area can be accessed from Nairobi-Thika - Kitui ,Nairobi – Machakos-Kitui roads, or from Mombasa –Kibwezi – Ikutha – Mutomo road. Mineral deposits in Kenya occur in different geological settings, such as those associated with Tertiary rocks (Turkana sapphire deposit). Most of the mineral deposits like iron ore located within the Neoproterozoic Mozambique orogenic belt have not been properly evaluated in geological and metallogenical context. This work intends to relate, evaluate and scientifically place the geological framework of iron deposits in Mutomo – Ikutha area of Kitui County, Kenya to the specific events within the litho and tectonothermal evolution of the Mozambique mobile Belt. A thorough investigation of the major and minor geological structures as well as metamorphism will be elucidated in the project area on their role in the formation of iron deposits. The establishment and economic and scientific investigation of iron deposits in the study area for purposes of mining and wealth creation in the region is of great importance in this research work. The application of the research to exploration and development of artisanal mining in Kenya will be successful in terms of prospecting at the regional and scale, by determining the lithological, geochemical and tectonic controls for the mineralization.
The Mozambique Belt has a long and complex history, marked by a succession of major tectonothermal events. This belt runs from Egypt through, Sudan, Ethiopia, Kenya, Uganda, Tanzania and ends in Mozambique. The methods to be used to achieve the aim of this research will include; geological, geochemical and geophysical investigations. Preliminary investigation will be carried out using remote sensed data. Laboratory analysis will include X-ray florescence, X-ray defractometry, and electron Microprobe. The data obtained will be analyzed using Oasis montaj software, Matlab and any other relevant software. The updated geological and structural maps will be compiled using Arc GIS software. This study is expected to provide comprehensive understanding of the tectonothermal scenario and its associated economic mineralization in the Mozambique belt.
WASWA AARONK, Nyamai CM, MATHU ELIUDM, Ichang’i DW. "
NEW FINDINGS OF THE TECTONIC CHARACTERISTICS OF THE EAST AFRICAN OROGENY IN THE KITUI – TAITA HILLS, S.E KENYA.". In:
35TH INTERNATIONAL GEOLOGICAL CONGRESS. CAPE TOWN, SOUTH AFRICA; 2016.
AbstractThe research area occurs within the Neoproterozoic Mozambique belt (NMB) in the south-eastern part of Kenya. An enigmatic problem within the polyphase and highly deformed NMB, is the lack of better understanding of the geology and tectonic structures. In order to decipher the aforementioned problem, this study undertook a geological study with the aim of having a better understanding of the petrology and tectono-metamorphic setting, in the study area. The research was carried out using geological field mapping and remote sense investigation. Samples collected in the field were analysed using XRF (X- ray fluorescence) and AAS (Atomic absorption spectrophotometry). The petrological investigation was carried out using petrographic microscopy. An investigation using remotely sensed data established prevalent geological structures, lithology and mineral alteration zones. This study has provided a comprehensive understanding of the tectono-thermal scenario. One of the key findings of this study is the realization that Kitui – Taita gneisses and migmatites represent a thick sequence of meta-sedimentary rocks. The entire protolith sequence was marked by the alternation of thin pelitic, psammitic and limey layers, together with minor thin basic meta-volcanic rocks that were deposited under deep marine conditions. The entire paragneissic sequence was subsequently subjected to multiple phases of successive deformation and metamorphism, which was accompanied by shearing, faulting and folding. Petrochemistry has revealed that Kitui – Taita hills area has remnants of Island alkali tholeiite and calc – alkaline Metabasalts. It has also revealed that Ikutha (south of Kitui) has huge deposit of apatite – iron of Kiruna type. Petrological studies have shown that Mutomo–Ikutha area occurs in an ophiolitic suite and mimics similar lithological and geochemical signatures as those reported in other ophiolite suites occurring in Kenya like in Moyale, Sekerr in West Pokot and Voi, SE Kenya. The field occurrence of this suite has been documented by the lithological and stratigraphic sequence of serpentinite, sheeted dykes, gabbro, limestone, tectonic mélange and remnants of pillow lava found in the type area. The field occurrence of this suite is an indication of the remnants of the obducted oceanic crust during the closure of the paleo- Mozambique Ocean and collision of the East and West Gondwanaland
WASIKE MRWEREISAAC. "
Prehistoric Settlements of Nairobi Area.". In:
MSc Thesis of University of New Hampshire. 79 pages. IBIMA Publishing; 1999.
AbstractA study that devised a modified method of reporting antibiotic sensitivity results was undertaken. Enterobacteriaceae and Gram positive cocci were tested for drug sensitivity by a disc diffusion method. Zones of bacterial growth inhibition were measured, dividing the isolates into four groups: the highly sensitive, the moderately sensitive, the slightly sensitive and the resistant ones. The slightly sensitive isolates were taken as indicators of antibiotic resistance acquisition. By that system, when more than 50% of the isolates fell into the slightly and resistant groups, that meant that the antibiotic concerned would be discontinued for some time until the bacteria reverted to being moderately sensitive. The study also provided a method of making antibiotic discs from local blotting papers, and a sample of a form on which antibiotic sensitivity results could be recorded was presented. The method is considered to be easy and very appropriate for developing countries in detecting gradual and abrupt acquisition of antibiotic resistance by bacteria.
WASIKE MRWEREISAAC. "
Europeans verses African: The land question. A case of Kikuyu people of Kenya. Catholic University of Eastern Africa.". In:
MSc Thesis of University of New Hampshire. 79 pages. IBIMA Publishing; 1997.
AbstractA study that devised a modified method of reporting antibiotic sensitivity results was undertaken. Enterobacteriaceae and Gram positive cocci were tested for drug sensitivity by a disc diffusion method. Zones of bacterial growth inhibition were measured, dividing the isolates into four groups: the highly sensitive, the moderately sensitive, the slightly sensitive and the resistant ones. The slightly sensitive isolates were taken as indicators of antibiotic resistance acquisition. By that system, when more than 50% of the isolates fell into the slightly and resistant groups, that meant that the antibiotic concerned would be discontinued for some time until the bacteria reverted to being moderately sensitive. The study also provided a method of making antibiotic discs from local blotting papers, and a sample of a form on which antibiotic sensitivity results could be recorded was presented. The method is considered to be easy and very appropriate for developing countries in detecting gradual and abrupt acquisition of antibiotic resistance by bacteria.
WASIKE MRWEREISAAC. "
The agony of AIDS in the rural areas: The case study of Kapenguria Division in West Pokot District.". In:
MSc Thesis of University of New Hampshire. 79 pages. IBIMA Publishing; 1998.
AbstractA study that devised a modified method of reporting antibiotic sensitivity results was undertaken. Enterobacteriaceae and Gram positive cocci were tested for drug sensitivity by a disc diffusion method. Zones of bacterial growth inhibition were measured, dividing the isolates into four groups: the highly sensitive, the moderately sensitive, the slightly sensitive and the resistant ones. The slightly sensitive isolates were taken as indicators of antibiotic resistance acquisition. By that system, when more than 50% of the isolates fell into the slightly and resistant groups, that meant that the antibiotic concerned would be discontinued for some time until the bacteria reverted to being moderately sensitive. The study also provided a method of making antibiotic discs from local blotting papers, and a sample of a form on which antibiotic sensitivity results could be recorded was presented. The method is considered to be easy and very appropriate for developing countries in detecting gradual and abrupt acquisition of antibiotic resistance by bacteria.
WASIKE MRWEREISAAC. "
A summary Report on Lukenya Ware.". In:
MSc Thesis of University of New Hampshire. 79 pages. IBIMA Publishing; 1999.
AbstractA study that devised a modified method of reporting antibiotic sensitivity results was undertaken. Enterobacteriaceae and Gram positive cocci were tested for drug sensitivity by a disc diffusion method. Zones of bacterial growth inhibition were measured, dividing the isolates into four groups: the highly sensitive, the moderately sensitive, the slightly sensitive and the resistant ones. The slightly sensitive isolates were taken as indicators of antibiotic resistance acquisition. By that system, when more than 50% of the isolates fell into the slightly and resistant groups, that meant that the antibiotic concerned would be discontinued for some time until the bacteria reverted to being moderately sensitive. The study also provided a method of making antibiotic discs from local blotting papers, and a sample of a form on which antibiotic sensitivity results could be recorded was presented. The method is considered to be easy and very appropriate for developing countries in detecting gradual and abrupt acquisition of antibiotic resistance by bacteria.
WASIKE MRWEREISAAC. "
An ethno-archaeological study of mortuary practices among the Pokot people.". In:
MSc Thesis of University of New Hampshire. 79 pages. IBIMA Publishing; 1996.
AbstractA study that devised a modified method of reporting antibiotic sensitivity results was undertaken. Enterobacteriaceae and Gram positive cocci were tested for drug sensitivity by a disc diffusion method. Zones of bacterial growth inhibition were measured, dividing the isolates into four groups: the highly sensitive, the moderately sensitive, the slightly sensitive and the resistant ones. The slightly sensitive isolates were taken as indicators of antibiotic resistance acquisition. By that system, when more than 50% of the isolates fell into the slightly and resistant groups, that meant that the antibiotic concerned would be discontinued for some time until the bacteria reverted to being moderately sensitive. The study also provided a method of making antibiotic discs from local blotting papers, and a sample of a form on which antibiotic sensitivity results could be recorded was presented. The method is considered to be easy and very appropriate for developing countries in detecting gradual and abrupt acquisition of antibiotic resistance by bacteria.
WASIKE MRWEREISAAC. "
African Indigenous Education: The case study of the Pokot circumcision ceremonies.". In:
MSc Thesis of University of New Hampshire. 79 pages. IBIMA Publishing; 1997.
AbstractA study that devised a modified method of reporting antibiotic sensitivity results was undertaken. Enterobacteriaceae and Gram positive cocci were tested for drug sensitivity by a disc diffusion method. Zones of bacterial growth inhibition were measured, dividing the isolates into four groups: the highly sensitive, the moderately sensitive, the slightly sensitive and the resistant ones. The slightly sensitive isolates were taken as indicators of antibiotic resistance acquisition. By that system, when more than 50% of the isolates fell into the slightly and resistant groups, that meant that the antibiotic concerned would be discontinued for some time until the bacteria reverted to being moderately sensitive. The study also provided a method of making antibiotic discs from local blotting papers, and a sample of a form on which antibiotic sensitivity results could be recorded was presented. The method is considered to be easy and very appropriate for developing countries in detecting gradual and abrupt acquisition of antibiotic resistance by bacteria.
WASIKE MRWEREISAAC. "
Economic Organisation of the Pre-colonial Pokot Society.". In:
MSc Thesis of University of New Hampshire. 79 pages. IBIMA Publishing; 1998.
AbstractA study that devised a modified method of reporting antibiotic sensitivity results was undertaken. Enterobacteriaceae and Gram positive cocci were tested for drug sensitivity by a disc diffusion method. Zones of bacterial growth inhibition were measured, dividing the isolates into four groups: the highly sensitive, the moderately sensitive, the slightly sensitive and the resistant ones. The slightly sensitive isolates were taken as indicators of antibiotic resistance acquisition. By that system, when more than 50% of the isolates fell into the slightly and resistant groups, that meant that the antibiotic concerned would be discontinued for some time until the bacteria reverted to being moderately sensitive. The study also provided a method of making antibiotic discs from local blotting papers, and a sample of a form on which antibiotic sensitivity results could be recorded was presented. The method is considered to be easy and very appropriate for developing countries in detecting gradual and abrupt acquisition of antibiotic resistance by bacteria.
WASIKE MRWEREISAAC. "
The Bukusu Peace Traditions and Sites.". In:
MSc Thesis of University of New Hampshire. 79 pages. IBIMA Publishing; 1999.
AbstractA study that devised a modified method of reporting antibiotic sensitivity results was undertaken. Enterobacteriaceae and Gram positive cocci were tested for drug sensitivity by a disc diffusion method. Zones of bacterial growth inhibition were measured, dividing the isolates into four groups: the highly sensitive, the moderately sensitive, the slightly sensitive and the resistant ones. The slightly sensitive isolates were taken as indicators of antibiotic resistance acquisition. By that system, when more than 50% of the isolates fell into the slightly and resistant groups, that meant that the antibiotic concerned would be discontinued for some time until the bacteria reverted to being moderately sensitive. The study also provided a method of making antibiotic discs from local blotting papers, and a sample of a form on which antibiotic sensitivity results could be recorded was presented. The method is considered to be easy and very appropriate for developing countries in detecting gradual and abrupt acquisition of antibiotic resistance by bacteria.
WASIKE MRWEREISAAC. "
Archaeological survey of the Athi-Kapiti Plains.". In:
MSc Thesis of University of New Hampshire. 79 pages. IBIMA Publishing; 1996.
AbstractA study that devised a modified method of reporting antibiotic sensitivity results was undertaken. Enterobacteriaceae and Gram positive cocci were tested for drug sensitivity by a disc diffusion method. Zones of bacterial growth inhibition were measured, dividing the isolates into four groups: the highly sensitive, the moderately sensitive, the slightly sensitive and the resistant ones. The slightly sensitive isolates were taken as indicators of antibiotic resistance acquisition. By that system, when more than 50% of the isolates fell into the slightly and resistant groups, that meant that the antibiotic concerned would be discontinued for some time until the bacteria reverted to being moderately sensitive. The study also provided a method of making antibiotic discs from local blotting papers, and a sample of a form on which antibiotic sensitivity results could be recorded was presented. The method is considered to be easy and very appropriate for developing countries in detecting gradual and abrupt acquisition of antibiotic resistance by bacteria.
WASIKE MRWEREISAAC. "
A preliminary report on archaeological excavation: A case study of Pastoral Neolithic sites at Lukenya Hills, University of Nairobi, Department of History.". In:
MSc Thesis of University of New Hampshire. 79 pages. IBIMA Publishing; 1997.
AbstractA study that devised a modified method of reporting antibiotic sensitivity results was undertaken. Enterobacteriaceae and Gram positive cocci were tested for drug sensitivity by a disc diffusion method. Zones of bacterial growth inhibition were measured, dividing the isolates into four groups: the highly sensitive, the moderately sensitive, the slightly sensitive and the resistant ones. The slightly sensitive isolates were taken as indicators of antibiotic resistance acquisition. By that system, when more than 50% of the isolates fell into the slightly and resistant groups, that meant that the antibiotic concerned would be discontinued for some time until the bacteria reverted to being moderately sensitive. The study also provided a method of making antibiotic discs from local blotting papers, and a sample of a form on which antibiotic sensitivity results could be recorded was presented. The method is considered to be easy and very appropriate for developing countries in detecting gradual and abrupt acquisition of antibiotic resistance by bacteria.
WASIKE MRWEREISAAC. "
The traditional pottery: A case study of the Pokot pottery and people of West Pokot District.". In:
MSc Thesis of University of New Hampshire. 79 pages. IBIMA Publishing; 1998.
AbstractA study that devised a modified method of reporting antibiotic sensitivity results was undertaken. Enterobacteriaceae and Gram positive cocci were tested for drug sensitivity by a disc diffusion method. Zones of bacterial growth inhibition were measured, dividing the isolates into four groups: the highly sensitive, the moderately sensitive, the slightly sensitive and the resistant ones. The slightly sensitive isolates were taken as indicators of antibiotic resistance acquisition. By that system, when more than 50% of the isolates fell into the slightly and resistant groups, that meant that the antibiotic concerned would be discontinued for some time until the bacteria reverted to being moderately sensitive. The study also provided a method of making antibiotic discs from local blotting papers, and a sample of a form on which antibiotic sensitivity results could be recorded was presented. The method is considered to be easy and very appropriate for developing countries in detecting gradual and abrupt acquisition of antibiotic resistance by bacteria.