Vernonia oil extracted by Soxhlet extraction from the seeds of Vernonia galamensis
ssp. nairobensis was reacted with 2-aminopyridine, 2-(aminomethyl)pyridine and 2-
(aminoethyl)pyridine using a 1:3 mole ratio of vernonia oil to amine at varied temperatures to give
the corresponding vernolamides. The expected vernolamide from the reaction of 2-aminopyridine
and the oil was not formed. N-(2-pyridinylmethyl)vernolamide and N-(2-
pyridinylethyl)vernolamide exhibited antibacterial activity that was shown to be higher against
gram-positive (Bacillus subtilis) than gram-negative (Escherichia coli) bacteria. The vernolamides
did not show any antifungal activity.