Effective biotransformation of Reactive Black 5 Dye Using Crude Protease from Bacillus Cereus Strain KM201428

Citation:
Wanyonyi WC, Onyari JM, Shiundu PM, Mulaa FJ. "Effective biotransformation of Reactive Black 5 Dye Using Crude Protease from Bacillus Cereus Strain KM201428." Energy Procedia. 2019;157:815-824.

Abstract:

Effective effluent treatment is a paramount step towards conserving the dwindling clean water resources. The present study describes the use of crude protease extract from Bacillus Cereus Strain KM201428 biotransformation of azo dye Reactive Black 5 (RB5). Batch experimental results displayed over 97% decolorization efficiency with initial dye concentration of 1.0 x 10-4M. The decolorization process was highly dependent on contact time, dye concentration and pH. The optimum contact time and pH for decolorization were 120 hours and pH 9 respectively at 25˚C. Biotransformation of RB5 dye was monitored using UV-Vis spectrophotometer and formed metabolites characterized by LC–QTOF-MS. Comparison of resultant LC–QTOF-MS chromatograms after decolorization confirmed complete cleavage of RB5 dye. First order kinetic fitted well with experimental data for different RB5 dye concentrations

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