Kikuvi G.M, Schwarz, S., Ombui, J.N., Mitema, E.S and Kehrenberg, C.(2007). Streptomycin and Chloramphenicol Resistance genes in Escherichia coli isolates from cattle, pigs and chicken in Kenya. Microbial Drug Resistance 31(1): 62-68.

Citation:
NYARONGI PROFOMBUIJ. "Kikuvi G.M, Schwarz, S., Ombui, J.N., Mitema, E.S and Kehrenberg, C.(2007). Streptomycin and Chloramphenicol Resistance genes in Escherichia coli isolates from cattle, pigs and chicken in Kenya. Microbial Drug Resistance 31(1): 62-68.". In: Microbial Drug Resistance 31(1): 62-68. University of Nairobi Press; 2007.

Abstract:

The aims of this study were to determine the genetic basis of streptomycin and chloramphenicol resistance in 30 Escherichia coli isolates from food animals in Kenya and the role of plasmids in the spread of resistance. Seven of the 29 streptomycin-reistant isolates harboured both the str A and StrB genes. Twenty one of the isolates had the strA, strB and aadAI genes. The strA gene was disrupted by a functional trimethoprim gene, dfrA14 in 10 of the 21 isolates harbouring the three streptomycin resistance genes. Physical linkage of intact strA and sul2 genes were found in two different plasmids from four isolates. Linkage of cassette-borne aadAI and dfrAI genes in class 1 integrons was found in two of the isolates. Chloramphenical resistance was due to the gene catA1 in  all the chloramphenicol resistant isolates. The strB, strA and catA1 genes were transferable by conjugation and this points to the significance of conjugative resistance plasmids in thespread and persistence of streptomycin and chloramphenicol resistance in food animals in Kenya.

Notes:

n/a

Website

UoN Websites Search