Kikuvi G. M., Ombui, J. N., Mitema, E. S., Schwartz, S and Kehrenberg C. (2008). Genetic basis, transferability and linkage of streptomycin and sulphonamide resistance genes in Escherichia coli from foods of animal origin in Kenya, Bull. Anim. Hlth. Prod.

Citation:
NYARONGI PROFOMBUIJ. "Kikuvi G. M., Ombui, J. N., Mitema, E. S., Schwartz, S and Kehrenberg C. (2008). Genetic basis, transferability and linkage of streptomycin and sulphonamide resistance genes in Escherichia coli from foods of animal origin in Kenya, Bull. Anim. Hlth. Prod.". In: nim. Hlth. Prod. Afr.56(1): 56-66. University of Nairobi Press; 2008.

Abstract:

The genetic basis and transferability of streptomycin and sulphonamide resistance was studied in 23 Escherichia coli isolates from food of animal origin in Kenya. Physical linkage of the streptomycin resistance gene strA with sulhonamide resistance gene Sul2 was investigated by PCR and confirmed by sequencing. Two small plasmids of 6kb (pSSGK1) and 8kb (pSSTGK1) identified by transformation to mediate resistance to at least streptomycin and sulphonamide were restricted in order to define their relatedness. Their restriction maps were compared to one another and with the maps of other plasmid from E. coli known to mediate these resistance properties.
Streptomycin resistance was based on the expression of the strA, strB and or aadA1 genes, while sulphonamide resistance was encoded by the sul2 or sul1 gene. The strA, strB and sul2 genes were transferable via conjugation and transformation. Hysically lnked sul2 and strA genes were present in both plasmid pSSGK1 and pSSTK1. Plasmids pssgk1 and psstgk1 were different from each other, but similar respectively to sulphonamide/streptomycin and sulphonamide/streptomycin/tetracycline resistance plasmids described previously in uropathgenic E. coli from humans.
Conjugation of plasmids encoding strptomycin and sulphonamide resistance may be one mechanism for the wide dissemination and persistence of these resistances among E. coli from food animals in Kenya. Physcal linkage of the plasmid-borne strA and sul2 genes would facilitate the spread of these genes by co-selection during selective pressure imposed by the use either of the two antimicrobials and highlights the need for the prudent use of streptomycin or sulphonamides in animal husbandry.

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