DIFFERENTIAL EFFECTS OF CHRONIC IRON DEFICIENCY ANAEMIA ON JUNCTIONAL AND LABYRINTHINE ZONES OF PLACENTA IN SPRAGUE DAWELY RAT

Citation:
Awad O, Malek A, Ogeng’o J. "DIFFERENTIAL EFFECTS OF CHRONIC IRON DEFICIENCY ANAEMIA ON JUNCTIONAL AND LABYRINTHINE ZONES OF PLACENTA IN SPRAGUE DAWELY RAT." Anatomy Journal of Africa. . 2017;6(1):840-846.

Abstract:

Iron deficiency anaemia causes adverse pregnancy outcome. Studies reveal its generalized effects on
histomorphometry of the placenta, without details on specific zones nor effect of gestational age. These data are
important for planning intervention. This study was, therefore, designed to describe the histomorphometric changes
associated with iron deficiency anaemia on placenta of albino rat. Fourty nine (49) Sprague – Dawely albino rats
were randomly separated into experimental and control groups. The experimental group was rendered anaemic by
removing 1.5 ml of blood per bleed on five alternate days. Placentas were collected on gestational days 17, 19 and
21. Five cubic milimetre segments were fixed in 10 % buffered formaldehyde solution; dehydrated in ethanol and
embedded in paraffin wax. Five micron thick sections were cut, deparaffinized and stained with Hematoxylin and
Eosin. Micrographs were taken using Leica ICC 50 digital photomicrographic camera attached to a computer at
magnification x40 and the thickness of the labyrinth and junctional zones measured. Student t- test was used to
compare values for the experimental and control groups. The labyrinth in the chronic anaemia group was thinner
than in the control group at gestational days 17, 19 and 21. The junctional zone, on the other hand, was consistently
thicker in anaemic than in the control animals. The difference in thickness of junctional zone varied with gestational
age. At gestational day 17, the zone was significantly thicker in the anaemic group (628.9 μ) than in the control
(381 μ). On day 19 and 21, however, the difference was not statistically significant. In conclusion, the effects of
chronic iron deficiency anaemia on the labyrinth differ from those on the junctional zone of the placenta. This
differential effect appears to depend on the function and gestational age. The decrease in thickness of the labyrinth
may be designed to maintain placental diffusion capacity while increased thickness of the junctional zone constitutes
a compensatory physical and nutritional adaptation to hypoxia.

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