Terajima J., B. Syuto and S. Kubo: Purification and characterization of neurotoxin produced by Clostridium botulinum type C 6813. Infect. Immun. 48, 312-317.

Citation:
O. PROFOCHANDAJAMES. "Terajima J., B. Syuto and S. Kubo: Purification and characterization of neurotoxin produced by Clostridium botulinum type C 6813. Infect. Immun. 48, 312-317.". In: First, Pan-African Conference on Bichemistry and Molecular Biology, September, 2-6, Nairobi, Kenya. Book of Abstract P. (181). East African Medical Journal; 1985.

Abstract:

C1 neurotoxin of Clostridium botulinum strains C-Stockholm (C-ST), C beta-Yoichi, C-468, CD6F, and C-CB19 and type D toxin of strains D-1873 and D-CB16 were purified by gel filtration, ion exchange, and affinity chromatographies. The purified toxins had di-chain structure made of heavy and light chains. The toxins of C beta-Yoichi, C-468, CD6F, and C-CB19 reacted with anti-C-ST heavy chain and anti-C-ST light chain in immunodiffusion tests and enzyme-linked immunosorbent assay, whereas D-CB16 toxin reacted with anti-D-1873 heavy chain and anti-D-1873 light chain. However, C-6813 toxin reacted with anti-D-1873 heavy chain and anti-C-ST light chain but not with anti-C-ST heavy chain or anti-D-1873 light chain immunoglobulin G. These results indicate common antigens in the heavy chains of C-6813 and D-1873 toxins and in the light chains of C-6813 and C-ST toxins. Further, they provide evidence for heterogeneity within type C1 toxin subunits.

Notes:

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