Anaerobic Digestion of Sewage Wastewaters with Sludge and Rumen Fluid

Mwazighe FM, Kamau GN, Mwaniki JM. "Anaerobic Digestion of Sewage Wastewaters with Sludge and Rumen Fluid." European International Journal of Science and Technology. 2014;Vol 3(6):61-66.


Anaerobic digestion was conducted at mesophilic (37oC) and thermophilic (55oC) conditions using sewage
wastewaters as the substrate and sludge and/or rumen fluid as the inoculum, with a view to optimize biogas
production. The substrate and inoculum were mixed in the ratios 1:1, 1:3, and 3:1 (volume by weight (where
sludge was used) or volume by volume (where rumen fluid was used).At mesophilic conditions for both
inocula, the 3:1 substrate/inoculum mixture produced the most biogas in a 24 hour period, with the rumen
mixture producing the highest yield (20 ml). At thermophilic conditions the 3:1 wastewater/sludge mixture
had the highest biogas yield (58 ml), whereas when rumen fluid was used as inoculum, the 1:3 mixture
produced the most biogas (66 ml). The thermophilic experiments using rumen as the inoculum were
repeated for a 10 day period and the 3:1 mixture achieved the maximum yield (140 ml) faster than the other
two (1:1 and 1:3 mixtures) indicating that the 3:1 substrate/inoculum ratio is the best.

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