Bio

Bio

Dr John Kaunga Muthee was born on 15th June 1963 and is married with three children. He is currently a lecturer in Food Animal Clinical and Production Medicine with special interest in dairy herd health. He graduated as a Veterinarian (BVM) from the Faculty of Veterinary Medicine, University of Nairobi in 1989. He joined the department of Clinical Studies in the same faculty as an assistant lecturer in august 1989.

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Publications


2013

Gakuya, DW, Itonga SM, Mbaria JM, Muthee JK, Musau JK.  2013.  Ethnobotanical survey of biopesticides and other medicinal plants traditionally used in Meru-Central district of Kenya. Journal of Ethno pharmacology. 145:547-553.

2011

Muthee, JK, Mbaria JM, Thaiyah AG, Karanja DN, Gakuya DW.  2011.  Clinical, Haematological, Biochemical and Pathological Manifestations of Sub-acute Toxicity of Nicandra physaloides (L) Gaertn in Calves.. Bulletin of Animal Health and Production in Africa. 59:17-24.
Muthee, JK, Gakuya DW, Mbaria JM, Kareru PG, Mulei CM, Njonge FK.  2011.  Ethnobotanical Study of Anthelmintic and Other Medicinal Plants Traditionally used in Loitoktok District of Kenya. Journal of Ethno pharmacology. 135:15-21.

2009

Muthee, JK, Mbaria JM, Thaiyah AG, Karanja DN, Gakuya DW.  2009.  Acute Toxicity Study of Nicandra physaloides(L) Gaertn in mice and cattle. The Kenya Veterinarian. 33:1-6.

2008

Muthee, JK.  2008.  Acute and Subacute Toxicity of Nicandra physaloides (L) Gaertn in Mice and Calves Respectively. , Nairobi Abstractabstract.pdf

The plant Nicandra physaloides (L) Gaertn, commonly known as the' apple of Peru' is a member of the solaneceae family. It has been widely associated with livestock poisoning in Kenya and elsewhere. The clinical signs reportedly associated with its poisoning are circling, tremors of the hind limbs, tachycardia, bloat, convulsions, coma and death. In the current study the acute toxicity was determined by intra-peritoneal injections of the aqueous extracts from different plant parts in a total of one hundred and fifty (150) white Swiss mice aged between to and 12 weeks and divided in groups of six (3 males and 3 females) for each dosage level. The median lethal dose (LDso) was then calculated by the method of Reed and Muench (1938). The subacute toxicity was determined by feeding five groups of two male Friesian calves each, aged between 8 and 10 months, at 0 (control), 4, 8, 16 and 32% levels of dried ground whole plant material in wheat bran for 14 weeks. The physiological parameters of rectal temperature, respiration, heart rate and ruminal motility were taken from all the calves every morning for the whole period of the experiment. The blood for hematology (5ml in EDTA) and biochemistry (l5ml without anticoagulant) was collected weekly via the jugular vene-puncture after thorough disinfection of the site with surgical spirit. LDso values for the leaf, fruit and whole plant extracts were 1.82, 2.58 and 3.62 g/kg body weight respectively, therefore, classifying the plant as slightly toxic according to Loomis (1978). The clinical signs showed by the mice were starry coat, slowed movements, fast respiration, gasping for air and leaping into the air before collapsing. The treated calves transiently exhibited muzzle drying, heart beat irregularity, loose feces, staggering gaits and lower growth rate than the control group. The activity of the enzyme gammaglutamyltransferase (GGT) and the mean corpuscular volume (MeV) were significantly lower (P0.05) between the treated and control groups in respect of the other assayed hematological (total protein. hemoglobin, red blood cells, packed cell volume, mean corpuscular hemoglobin concentration, white blood cells, lymphocytes and neutrophils) and biochemical (aspartate aminotransferase, creatinine and blood urea nitrogen) parameters. There were no mortalities, no gross or histopathological lesions in all the groups. The possible reasons for the difference in behaviour between the calves in this experiment and the suspected natural cases were thought to be due to the variations in animal susceptibility or even plant toxin content. It is concluded that the plant Nicandra physaloides growing around the Kabete areas of Kenya may contain toxic phytochemicals that may cause poisoning in livestock if consumed. Nicandra physaloides is known to contain glycosides, steroids and alkaloids from earlier studies. More studies are necessary to determine the nature of these phytotoxins and their exact mode of action. Meanwhile livestock keepers are advised to control this plant in their pastures and avoid its consumption by animals.

2005

Muthee, JK, D GW, Nduhiu J.  2005.  Preliminary investigations on the status of bovine mastitis in a medium scale dairy farm in Kabete area, Kenya. The Kenya Veterinarian 29:57-59. The Kenya Veterinarian. 29:57-59.: Kenya Electricity Generating Co./IAEA AbstractWebsite

The purpose of this investigation was to establish the mastitis status in the dairy herd of 110 cows (genetically heterogeneous Bos Taurus breeds). All the cows were examined by physical inspection of the udder and California Mastitis Test (CMT) carried out on milk from the quarters of the 89 cows in milk. A total of 37 milk samples were collected from affected quarters of 36 cows, which had visibly abnormal secretion or tested positive on CMT, and taken for bacteriological culture and sensitivity tests. The outcome was a prevalence of 40.4 % based on the cows in milk and 18.4% based on their quarters. From the 37 samples that were cultured, 36 had abundant bacterial growth. Of the bacteria isolated, 45 % were Corynebacteria, 30.0 % Staphylococci, 22.5% Streptococci and 2.65% Pseudomonas. Of the twelve antibacterial compounds used in the sensitivity tests, the bacteria were most sensitive to ampi-clox and cefaclor at 98% and lest sensitive to sulphamethoxazole and cotrimoxazole both at 2%. Pseudomonas was only sensitive to gentamycin, norfloxacin and tetracycline. From this investigation the prevalence of mastitis in the herd is fairly high and there is need for a comprehensive monitoring and control program for udder health in this herd

1996

Anonymous.  1996.  Traditional remedies for tick-borne cattle diseases in Kenya. A field manual of traditional animal health care practices. , Nairobi: ITDG and IIRR

1991

Maina, AK, Muthee JK, Tsuma VT.  1991.  An outbreak of progressive cataracts of an undetermined cause in a herd of dairy cows. The Indian Journal of Animal Sciences vol. 61 no. 11. The Indian Journal of Animal Sciences. 61(11): Kenya Electricity Generating Co./IAEA AbstractWebsite

The purpose of this investigation was to establish the mastitis status in the dairy herd of 110 cows (genetically heterogeneous Bos Taurus breeds). All the cows were examined by physical inspection of the udder and California Mastitis Test (CMT) carried out on milk from the quarters of the 89 cows in milk. A total of 37 milk samples were collected from affected quarters of 36 cows, which had visibly abnormal secretion or tested positive on CMT, and taken for bacteriological culture and sensitivity tests. The outcome was a prevalence of 40.4 % based on the cows in milk and 18.4% based on their quarters. From the 37 samples that were cultured, 36 had abundant bacterial growth. Of the bacteria isolated, 45 % were Corynebacteria, 30.0 % Staphylococci, 22.5% Streptococci and 2.65% Pseudomonas. Of the twelve antibacterial compounds used in the sensitivity tests, the bacteria were most sensitive to ampi-clox and cefaclor at 98% and lest sensitive to sulphamethoxazole and cotrimoxazole both at 2%. Pseudomonas was only sensitive to gentamycin, norfloxacin and tetracycline. From this investigation the prevalence of mastitis in the herd is fairly high and there is need for a comprehensive monitoring and control program for udder health in this herd

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