Publications

Found 56 results

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2013
and Gathumbi, J.K. KKSG. "Traces of opiates in Shisha collected in Nairobi, Kenya.". In: National Conference on Alcohol and Drug Abuse. Kasarani stadium; 2013.
and 1. J. K. Kibugu, J.N. Makumi KNGMMMJMJJN. Effects of mycotoxins on the pathogenesis of Trypanosoma brucei rhodesiense in mice. . Panafric Hotel, Nairobi, Kenya ; 2013.
2012
Gathumbi J.K. "Overview of sampling and analytical methods for mycotoxins. .". In: COMESA Regional training on food safety/aflatoxin control for Regionally harmonized sampling and laboratory procedures. KEPHIS Hqs, Nairobi, Kenya.; 2012.
Muthomi J.W., Mureithi B.K. C’wa GMGNJK. "Aspegillus species and Aflatoxin B1 in soil, Maize grain and flour samples from semi arid and humid regions of Kenya." international Journal of AgriScience. . 2012;2(1):22-34.
2010
K DRGATHUMBIJAMES. "J.M. Nguta, J.M. Mbaria, D.W. Gakuya, P.K. Gathumbi, S.G. Kiama (2010). Antimalarial herbal remedies of Msambweni Kenya. J Ethnopharmacol. 128: 424-432.". In: J Ethnopharmacol. 128: 424-432. University of nairobi; 2010. Abstract
Abstract in Bellamy, M. and B. Greenshields (eds), Issues in Agricultural Development: Sustainability and Cooperation. IAAE Occasional Paper No. 6. Dartmouth Publishing Co. Ltd, Aldershot.
K DRGATHUMBIJAMES. "John B. Githiori and Peter K Gathumbi (2010 ) Ethnoveterinary plants used in East Africa, In: Ethnobotanical Medicines of Animals Health Taylor & Francis Group LLC Boca Raton, USA (In Press).". In: J Ethnopharmacol. 128: 424-432. University of nairobi; 2010. Abstract
Abstract in Bellamy, M. and B. Greenshields (eds), Issues in Agricultural Development: Sustainability and Cooperation. IAAE Occasional Paper No. 6. Dartmouth Publishing Co. Ltd, Aldershot.
2009
and Kibugu J.K., Makumi J.N. NKGMJJNJM. "Aggravation of pathogenesis mediated by aflatoxin B1 in mice infected with Trypanosoma brucei rhodesiense." Protozool. Res. 19. 2009:24-33.
Kibugu J.K., Ngeranwa J.J. MGKMMMJNJK. "Aggravation of pathogenesis mediated by ochratoxin A in mice infected with Trypanosoma brucei rhodesiense. ." Parasitology.. 2009;136(3):273-281.
2008
and 4. Muthomi, J. W. RN’u NGGMJK. "Fusarium head blight in Kenya and possible management strategies. .". In: 10th International Fusarium Workshop and the Fusarium Genomics Workshop. Alghero (Sardinia), Italy. ; 2008.
and Muthomi, J. W. NGC’waLNJK. "Mycotoxin-producing fungi and aflatoxin incidence in maize from Machakos, Kenya. .". In: 11th KARI Biennial Scientific Conference . Nairobi, Kenya. ; 2008.
Riungu GM, Muthomi JW, Narla RD, Gathumbi JK. "Fusarium head blight, DON and Fusarium contamination of Wheat and Maize.". 2008.
and Muthomi, J. W. RN’u NGWGMJK. "Head blight of wheat in Kenya and contamination of grain with mycotoxin producing Fusarium species." Journal of Plant Sciences . 2008;3(1):52-60.
and Riungu G. M., J. W. Muthomi NWGRDJM. "Management of Fusarium head blight of wheat and deoxynivalenol accumulation using antagonistic microorganisms." Plant Pathology Journal . 2008;7(1):13-19.
and Muthomi, J. W. N’u GMWJKJK. "The occurrence of Fusarium species and mycotoxins in Kenyan wheat." Crop Protection . 2008;27(8):1215-1219.
2007
and Muthomi, J. W. HN’u GHJKJ. "Occurrence of Fusarium head blight – causing pathogens and mycotoxins in Kenyan wheat." Journal of Plant Diseases and Protection. 2007;114(9).
2006
and Muthomi, J. W. N’u GJKJJ. "Occurrence of wheat head blight-causing Fusarium species and associated trichothecene mycotoxins in Kenyan wheat.". In: 10th KARI Biennial Scientific Conference. Nairobi, Kenya.; 2006.
and Muthomi, J. W. HN’u GHJKJK. "Occurrence of Fusarium head blight-causing pathogens and mycotoxins in Kenyan wheat. .". In: Tropentag. University of Bonn, Germany ; 2006.
Gathumbi JK, Ndung'u JK, Hindorf H, Muthomi JW. "Occurrence of Fusarium Head Blight–causing Pathogens and Mycotoxins in Kenyan Wheat.". 2006. Abstract

Fusarium head blight is a devastating disease of wheat and other small-grain cereals, causing grain yield reduction, reduced quality and mycotoxin contamination. This study was conducted in two districts of Kenya to determine the incidence of Fusarium species and mycotoxin contamination in freshly harvested wheat. A survey was carried out during the 2004 growing season in different agro-climatic zones to determine the presence of Fusarium head blight and weather conditions during the critical stages of wheat growth. Fungal contamination was determined by isolation on agar media while mycotoxin analysis was by direct competitive ELISA. Fusarium head blight was reported by 81% of the farmers and wet conditions were prevalent during anthesis. The wheat grain samples were highly contaminated with fungi, especially Epicoccum, Alternaria and Fusarium species. The mean Fusarium infection rate varied from 13 to 18%, with the major head blight – causing species being F. poae, F. graminearum, F. equiseti, and F. avenaceum. Fusarium poae, F. chlamydosporum and F. oxysporum were the most prevalent in all the agro-ecological zones while F. graminearum was isolated in 6 out of the 9 agro-ecological zones. Most grain samples were contaminated with mycotoxins deoxynivalenol, T-2 toxin, zearalenone and aflatoxin B1. The most prevalent mycotoxin was T-2 (86% of the samples) followed by deoxynivalenol (59%), zearalenone (53%), and aflatoxin B1 (52%). The maximum mycotoxin concentration was 302mg/kg, 95.8mg/kg, 65.7mg/kg and 6.9mg/kg for deoxynivalenol, zearalenone, T-2 toxin and aflatoxin B1, respectively. The incidence and levels of the mycotoxins varied depending on the agro-ecological zone. Samples with high proportion of total Fusarium infection contained higher deoxynivalenol and T-2 toxin levels. Co-occurrence of deoxynivalenol, T-2 toxin and zearalenone was fund in up to 35% of the samples. The results suggested the presence of Fusarium head blight in Kenya and associated mycotoxin contamination, though at low but significant levels. The presence of the different mycotoxins, though at low levels, could pose chronic adverse health effects to human and livestock fed on the contaminated wheat products.

2005
K DRGATHUMBIJAMES. "Gathumbi J.K (2005). An overview of methods for detection of aflatoxins in foods.". In: Proceedings of a WHO Experts group meeting on aflatoxins and health held on 24 . University of nairobi; 2005. Abstract
Abstract in Bellamy, M. and B. Greenshields (eds), Issues in Agricultural Development: Sustainability and Cooperation. IAAE Occasional Paper No. 6. Dartmouth Publishing Co. Ltd, Aldershot.
2004
K DRGATHUMBIJAMES. "Mbugua, S.M. and Gathumbi J.K. (2004). The contamination of Kenyan lager beers Fusarium mycotoxins. Journal of The Institute of Brewing 110: 227-229.". In: Proceedings of a WHO Experts group meeting on aflatoxins and health held on 24 . University of nairobi; 2004. Abstract
Abstract in Bellamy, M. and B. Greenshields (eds), Issues in Agricultural Development: Sustainability and Cooperation. IAAE Occasional Paper No. 6. Dartmouth Publishing Co. Ltd, Aldershot.
2003
and Mutitu, E.W. NOGRDHJ. "Causes of ear rot of maize with mycotoxin implication in Eastern and Central Kenya. .". In: 6th Biennial Conference of the African Crop Science Society.; 2003.
10. Gathumbi JK. "Mycotoxin status in Kenya. .". In: Kenya Veterinary Association Nairobi Branch Scientific Conference . Nairobi, Kenya ; 2003.
and T.A. GNJK. "An immunochemical survey of ochratoxin A in swine serum in central Kenya." Bulletin of Animal Health and Production in Africa . 2003;51:64-66.
status in Kenya. M. "Mycotoxin status in Kenya.".; 2003.
K DRGATHUMBIJAMES. "Gathumbi J.K. and Ngatia T.A. (2003). An immunochemical survey of ochratoxin A in swine serum in central Kenya. Bulletin of Animal Health and Production in Africa 51:64-66.". In: Proceedings of a WHO Experts group meeting on aflatoxins and health held on 24 . University of nairobi; 2003. Abstract
Abstract in Bellamy, M. and B. Greenshields (eds), Issues in Agricultural Development: Sustainability and Cooperation. IAAE Occasional Paper No. 6. Dartmouth Publishing Co. Ltd, Aldershot.
K DRGATHUMBIJAMES. "Gathumbi, J. K., Usleber, E., Ngatia, T.A., Kangethe, E.K. and M.". In: Proceedings of a WHO Experts group meeting on aflatoxins and health held on 24 . University of nairobi; 2003. Abstract
Abstract in Bellamy, M. and B. Greenshields (eds), Issues in Agricultural Development: Sustainability and Cooperation. IAAE Occasional Paper No. 6. Dartmouth Publishing Co. Ltd, Aldershot.
K DRGATHUMBIJAMES. "Gathumbi, J.K. (2003). Mycotoxin status in Kenya.". In: A paper presented at the Kenya Veterinary Association Nairobi Branch Scientific Conference and Annual General Meeting held in Nairobi on 26th February, 2003. University of nairobi; 2003. Abstract
Abstract in Bellamy, M. and B. Greenshields (eds), Issues in Agricultural Development: Sustainability and Cooperation. IAAE Occasional Paper No. 6. Dartmouth Publishing Co. Ltd, Aldershot.
K DRGATHUMBIJAMES. "Mutitu, E.W., Narla, R.D., Oduor, H. and Gathumbi J.K., (2003). Causes of ear rot of maize with mycotoxin implication in Eastern and Central Kenya.". In: 6th Biennial Conference of the African Crop Science Society held in on October 12-17th, 2003. University of nairobi; 2003. Abstract
Abstract in Bellamy, M. and B. Greenshields (eds), Issues in Agricultural Development: Sustainability and Cooperation. IAAE Occasional Paper No. 6. Dartmouth Publishing Co. Ltd, Aldershot.
2002
and Gathumbi, J.K. WMMJEWJ. "Application of rapid immunoassays in a survey of mycotoxins (aflatoxins, deoxynivalenol and fumonisins) in foods and feeds. .". In: Faculty of Veterinary Medicine Bi-annual Conference. Nairobi, Kenya; 2002.
Gathumbi JK. "Immunological determination of mycotoxins in animal feeds: principles and application.". In: 2nd Kenya Livestock Technicians Association Scientific Conference . KARI, Nairobi, Kenya ; 2002.
Karanja DN;, Ngatia TA;, Gathumbi JK. "Edema Disease Of Swine: A Major Disease Problem In Kenya. .".; 2002.
K DRGATHUMBIJAMES. "Gathumbi J.K. (2002). Immunological determination of mycotoxins in animal feeds: principles and application.". In: A paper presented at the 2nd Kenya Livestock Technicians Association Scientific Conference held in KARI on 10-12th September, 2002. University of nairobi; 2002. Abstract
Abstract in Bellamy, M. and B. Greenshields (eds), Issues in Agricultural Development: Sustainability and Cooperation. IAAE Occasional Paper No. 6. Dartmouth Publishing Co. Ltd, Aldershot.
K DRGATHUMBIJAMES. "Gathumbi J.K., Wakhisi, J., Mbuthia, E.W. and Mosonik, J.S., (2002). Application of rapid immunoassays in a survey of mycotoxins (aflatoxins, deoxynivalenol and fumonisins) in foods and feeds.". In: A paper presented at the Faculty of Veterinary Medicine Bi-annual Conference held in Nairobi on 7-9th August, 2002. University of nairobi; 2002. Abstract
Abstract in Bellamy, M. and B. Greenshields (eds), Issues in Agricultural Development: Sustainability and Cooperation. IAAE Occasional Paper No. 6. Dartmouth Publishing Co. Ltd, Aldershot.
K DRGATHUMBIJAMES. "Salah, A.M., Gathumbi, J., Vierling W. (2002). Inhibition of intestinal motility by methanol extracts of Hibiscus sabdariffa L. (malvoceae) in rats. Phytotherapy Research 163: 283-285.". In: 6th Biennial Conference of the African Crop Science Society held in on October 12-17th, 2003. University of nairobi; 2002. Abstract
Abstract in Bellamy, M. and B. Greenshields (eds), Issues in Agricultural Development: Sustainability and Cooperation. IAAE Occasional Paper No. 6. Dartmouth Publishing Co. Ltd, Aldershot.
K DRGATHUMBIJAMES. "Salah, A.M., Gathumbi, J., Vierling W. and Wagner, H. (2002). Estrogenic and cholinergic properties of methanol extract of Ruellia praetermissa Sceinf.ex.Lindau (Acanthaceae) in female rats. Phytomedicine 9: 52-55.". In: 6th Biennial Conference of the African Crop Science Society held in on October 12-17th, 2003. University of nairobi; 2002. Abstract
Abstract in Bellamy, M. and B. Greenshields (eds), Issues in Agricultural Development: Sustainability and Cooperation. IAAE Occasional Paper No. 6. Dartmouth Publishing Co. Ltd, Aldershot.
2001
Gathumbi JK, Usleber E, Martlbauer E. "Production of ultrasensitive antibodies against aflatoxin B 1.". 2001. Abstract

Aims: To produce speciÆc antibodies against the haptenic fungal toxin aØatoxin B1 (AFB1) and apply these antibodies in immunochemical assays for aØatoxins. Methods and Results: Rabbits were immunized using an AFB1 -bovine serum albumin conjugate and serum titres determined by double-antibody enzyme immunoassay. High titres of antibodies with very high afÆnity for AFB 1 were obtained 15 and 4 weeks after the initial immunization and the Ærst booster immunization respectively. The antibodies were employed in enzyme immunoassay (EIA) and immunoafÆnity chromatography (IAC) methods for aflatoxins. With a detection limit of 15 · 8pgml)1 for AFB1 , the EIA employing these antibodies is the most sensitive test for AFB1 described so far. In IAC columns, these antibodies provided high binding capacity for all major aØatoxins, including AFB1 , AFB2 , AFG1 and AFG2 Conclusions: The antibodies described here are useful for the analysis of trace levels of aflatoxins. SigniÆcance and Impact of the Study: Polyclonal antibody-based EIA and IAC methods for aflatoxin analysis offer a suitable alternative to the more expensive monoclonal antibody- based methodS.

and Gathumbi, J. K. UMEE. "Production of ultrasensitive antibodies against aflatoxin B1." Letters in Applied Microbiology . 2001;32:349-351.
K DRGATHUMBIJAMES. "Gathumbi, J. K., Usleber, E. and M.". In: A paper presented at the 2nd Kenya Livestock Technicians Association Scientific Conference held in KARI on 10-12th September, 2002. University of nairobi; 2001. Abstract
Abstract in Bellamy, M. and B. Greenshields (eds), Issues in Agricultural Development: Sustainability and Cooperation. IAAE Occasional Paper No. 6. Dartmouth Publishing Co. Ltd, Aldershot.
K DRGATHUMBIJAMES. "Gathumbi, J.K. (2001). Application of enzyme immunochemical and immunohistochemical methods in the diagnosis of aflatoxicosis in animals. Ph.D Thesis. University of Nairobi.". In: A paper presented at the 2nd Kenya Livestock Technicians Association Scientific Conference held in KARI on 10-12th September, 2002. University of nairobi; 2001. Abstract
Abstract in Bellamy, M. and B. Greenshields (eds), Issues in Agricultural Development: Sustainability and Cooperation. IAAE Occasional Paper No. 6. Dartmouth Publishing Co. Ltd, Aldershot.
2000
and Gathumbi, J. K. UMEE. "Rapid extraction and immunological determination of aflatoxin in chicken liver tissues. .". In: XXI World Poultry Congress. Montréal, Canada ; 2000.
Gathumbi, J.K. and Bebora LC. "The occurrence of aflatoxin in poultry tissues collected in Nairobi, Kenya." Bulletin of Animal Health and Production in Africa . 2000;48:61-62.
K DRGATHUMBIJAMES. "Gathumbi, J. K., Usleber, E. and M.". In: tissues. Poster presentation at the XXI World Poultry Congress to be held in Montr. University of nairobi; 2000. Abstract
Abstract in Bellamy, M. and B. Greenshields (eds), Issues in Agricultural Development: Sustainability and Cooperation. IAAE Occasional Paper No. 6. Dartmouth Publishing Co. Ltd, Aldershot.
K DRGATHUMBIJAMES. "Gathumbi, J.K. and Bebora, L.C. (2000). The occurrence of aflatoxin in poultry tissues collected in Nairobi, Kenya. Bulletin of Animal Health and Production in Africa 48: 61-62.". In: A paper presented at the 2nd Kenya Livestock Technicians Association Scientific Conference held in KARI on 10-12th September, 2002. University of nairobi; 2000. Abstract
Abstract in Bellamy, M. and B. Greenshields (eds), Issues in Agricultural Development: Sustainability and Cooperation. IAAE Occasional Paper No. 6. Dartmouth Publishing Co. Ltd, Aldershot.
1996
and Gathumbi, J.K. BMNLCDJ. "Mycological examination of poultry feeds used in Nairobi, Kenya." Bulletin of Animal Health and Production in Africa . 1996;44:19-22.
K DRGATHUMBIJAMES. "Gathumbi, J.K., Bebora, L.C., Muchiri, D. J. and Ngatia, T.A. (1996). Mycological examination of poultry feeds used in Nairobi, Kenya. Bulletin of Animal Health and Production in Africa 44: 19-22.". In: tissues. Poster presentation at the XXI World Poultry Congress to be held in Montr. University of nairobi; 1996. Abstract
Abstract in Bellamy, M. and B. Greenshields (eds), Issues in Agricultural Development: Sustainability and Cooperation. IAAE Occasional Paper No. 6. Dartmouth Publishing Co. Ltd, Aldershot.
1995
and Gathumbi, J.K. BMNLCDJ. "A survey of mycotoxins in poultry feeds used in Nairobi, Kenya. ." Bulletin of Animal Health and Production in Africa . 1995;43:243-245.
K DRGATHUMBIJAMES. "Gathumbi, J.K., Bebora, L.C., Muchiri, D. J. and Ngatia, T.A. (1995). A survey of mycotoxins in poultry feeds used in Nairobi, Kenya. Bulletin of Animal Health and Production in Africa 43: 243-245.". In: tissues. Poster presentation at the XXI World Poultry Congress to be held in Montr. University of nairobi; 1995. Abstract
Abstract in Bellamy, M. and B. Greenshields (eds), Issues in Agricultural Development: Sustainability and Cooperation. IAAE Occasional Paper No. 6. Dartmouth Publishing Co. Ltd, Aldershot.
1993
Gathumbi JK. A survey of mycotoxigenic fungi and mycotoxins in poultry feeds. . Nairobi.: University of Nairobi.; 1993.
K DRGATHUMBIJAMES. "Gathumbi, J.K. (1993). A survey of mycotoxigenic fungi and mycotoxins in poultry feeds. MSc Thesis. University of Nairobi.". In: tissues. Poster presentation at the XXI World Poultry Congress to be held in Montr. University of nairobi; 1993. Abstract
Abstract in Bellamy, M. and B. Greenshields (eds), Issues in Agricultural Development: Sustainability and Cooperation. IAAE Occasional Paper No. 6. Dartmouth Publishing Co. Ltd, Aldershot.

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