Publications

Found 54 results

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2018
Mbali KK, Mutembei HM MJK. "BENEFITS OF ADOPTING BIOGAS TECHNOLOGY IN." International Journal of Innovative Research and Knowledge. 2018;3(3):87-92.mutembei-mbali-benefits-2018.pdf
M’Ikiugu MH. "Demographic Factors that Affect Adoption of Biogas Technology in Kiambu County, Kenya." INTERNATIONAL JOURNAL OF INNOVATIVE RESEARCH AND KNOWLEDGE. 2018;3(1):48-57.mbali_et_al._2018-_mutemebi-_muthee.pdf
Kivisi Mbali, Mutembei Henry MJ. "Governance Aspects on Adoption of Biogas Technology in." International Journal of Innovative Research and Knowledge. 2018;3(3):81-86.mutembei-mbali-governance-2018.pdf
2017
2016
Okell CN, Mutembei HM MJCABRJRVKLRN. "Anthelmintic administration to small ruminants in emergency drought responses:." Journal of Tropical Animal Health and Production. 2016;48(3):493-500.claire-mutembei_et_al._2016-_phd_paper.pdfclaire-mutembei_et_al._2016-_phd_paper.pdf
2015
J M, HM M, VT Tsuma ERM. "characterization of follicular dynamics in the Kenyan Boran Cow." Inter J Vet Sci. 2015;4(4):206-210.mutembei-muraya_2015-_dynamics.pdf
Lawrence FG, HM Mutembei LAAMOJJ. "Constraints to use of breeding services in Kenya." Inter J Vet Sci. 2015;4(4):211-215.mutembei-gacheri_2015-breeding_constraints.pdf
Lawrence FG, Mutembei H, Job Lagat, John Mburu OM. "A cost-benefit analysis of usage of sexed invitro fertilization embryo transfer in Kenya." Journal of Agricultural Science and Food Technology. 2015;1(4):53-58.cost_benefit_paper_2015.pdf
Mutembei HM, Tsuma VT MBSMJ. "Manual on Technical aspects of Bovine in-vitro embryo production and embryo transfer." The Kenya Veterinarian Journal. 2015;39(1):1-16.
Mutembei HM, Cook EA MMDNAKMCMJJA. "A One-Health Approach to Control the Incidence of Rabies in the Slums of Nairobi." Journal of Medical and Biological Science Research. 2015;1(9):130-133.mutembei_et_al__2015-one_health_rabies_control.pdf
Ndeke AN, Mutembei HM TVTMER. "Reproductive performance of the Galla and Toggenburg goats and their crosses in Mwingi sub-county of Kenya." Journal of Agricultural Science and Food Technology. 2015;1(6):78-83.mutembei-ndeke_2015_-_ngala_goat_reproduction.pdf
2014
Frances A, et al. "One Health- the WSPA Approach.". 2014.
2013
2012
2011
Mutembei, HM; Camargo; Viana JHM;, Muasa B;, Tsuma VT;, Origa RA;, Camargo; Viana, Okeyo AM. In vitro embryo production using Boran (Bos indicus) oocytes in Kenya.; 2011.Website
2010
Aleri JW;, Mutembei HK;, Mulei CM;, Mbugua SM;, Gakombe JW. "Retrospective Study On Canine Infertility In Nairobi And Its Environs (2010)."; 2010.
Hoffmann B, Rostalski A, Mutembei HM, Goericke-Pesch S. "Testicular steroid hormone secretion in the boar and expression of testicular and epididymal steroid sulphatase and estrogen sulphotransferase activity.". 2010. Abstract

BACKGROUND: Spermatogenesis and epididymal function depend on testicular steroids with estrogens being important regulatory factors. However, testicular estrogen secretion shows distinct species specificities, with the boar being characterized by the production of high amounts of estrone [E1] and estronesulphate [E1S]. As the boar testis also expresses estrogen sulphatase [StS] and sulphotransferase [EST] the present paper is based on the hypothesis that local availability of biologically active estrogens results from an interplay between estrogen synthesis and local activities of StS and EST. METHODS: Blood was collected during castration of 37 boars, aged between 98 (peripubertal) to 2 793 (old sexually mature) days, from the testicular vein and artery and peripheral circulation; E1, E1S, testosterone [T] and progesterone [P] were determined by established RIA-procedures. Similarly seminal plasma from 21 sexually mature boars was assessed. StS- and EST-activity were determined in testicular- and epididymal homogenates of 3 sexually mature boars (200 d) using (3)H-E1S resp. (3)H-E1 as substrate. Immunohistochemistry [IHC] was applied to locate EST- expressing cells in testes in relation to age and in the epididymis of 3 mature boars. RESULTS: Hormone data showed a high variability. A significant age dependent increase was only observed for E1 and E1S in the peripheral circulation with absolute values being highest for E1S (5-60 nmol/l), followed by T (2.6-14 nmol/l), P (0.5-1.48 nmol/l) and E1 (0.24-0.84 nmol/l). Testicular vein concentrations always exceeded those in the testicular artery with the differences being significant for E1 and P, group 1. Concentrations in the testicular artery and peripheral vein plasma were not different but higher (p<0.0001) than those in seminal plasma with the exception of E1. StS activity was higher (p<0.001) in the testis than the epididymis. EST activity was high in epididymal homogenates and at the level of detection in testis homogenates. IHC located EST in virtually all epididymal epithelial cells. In the testis the number of positive staining Leydig cells decreased (p<0.05) from 72% in the premature to 57% in the mature boars. CONCLUSION: The provision of biologically active estrogens to the testicular and epididymal compartment is controlled by a complex regulatory system, with the sulphatase pathway being an important component. P is a secretory product of the testis, E1 and E1S are not actively enriched in seminal plasma.

2009
Fridah L;G, Isabelle B;, Job L;, John M;, Henry M;, Joshua A;O, Okeyo AM. "A cost-benefit analysis of seced in-vitro fertilization embryo transfer in Kenya."; 2009.
2008
HENRY DRM&#39;IKIUGUMUTEMBEI, HENRY DRM&#39;IKIUGUMUTEMBEI, HENRY DRM&#39;IKIUGUMUTEMBEI, HENRY DRM&#39;IKIUGUMUTEMBEI, HENRY DRM&#39;IKIUGUMUTEMBEI. "Canine prostaglandin E2 synthase (PGES) and its receptors (EP2 and EP4): Expression in the corpus luteum during dioestrus.". In: Animal Reproduction Science 109 (2008) 319. Elsevier; 2008.
2006
HENRY DRM&#39;IKIUGUMUTEMBEI. "HM Mutembei, S Pesch, M Kowalewski, G Schuler, B Ugele,W Song and B Hoffmann 2006: Expression of Estrogen sulfotransferase in the testis of Immature and Mature boars.Reprod Dom Animal 41 (Suppl.1): 24.". In: Animal Reproduction Science 109 (2008) 319. Elsevier; 2006. Abstract
estrone sulfate (E1S) by far exceeding those of free estrone (E1). Expression of estrogen receptors has been observed in Leydig-, Sertoli- and germ cells. This suggests a paracrine and/or autocrine activity of the Leydig cell derived estrogens. In vitro studies with boar testicular tissue have demonstrated activity of both StS and EST. The bioavailability of free estrone might result from the interplay of both enzymes. We investigated for the localisation of EST enzyme in the testes of immature and mature boars by immunohistochemistry using samples from animals aged 50, 100, 150, 200 and 250 days. Rabbit polyclonal antibodies against human EST alongside negative controls with the rabbit serum were used. In all age groups both the EST was localised, with their signals restricted to the cytoplasm of the Leydig cells. The intensity of EST decreased as the boars matured. RT-PCR confirmed testicular expression of EST, in situ hybridisation confirmed localisation of EST in the Leydig cells. For a functional interpretation further studies are necessary. However, in respect to the lower activity of EST determined in the testis compared to the epididymis and the local circulatory system, rather the epididymis than the testis seems to be the origin of the high E1Sconcentrations determined in the testicular vein plasma.
HENRY DRM&#39;IKIUGUMUTEMBEI. "MP Kowalewski, HM Mutembei, B Hoffmann 2006: Expression of Prostaglandin sythetase in the corpus luteum of the dog. Reprod Dom Animal 41 (Suppli.1): 21.". In: Animal Reproduction Science 109 (2008) 319. Elsevier; 2006. Abstract
PGFR plays a critical role in determining luteal sensitivity to the luteolytic actions of PGF2a. In the dog the luteal life span can be terminated by exogenous application of PGF2a or its analogues. However, relatively high dosages or repeated treatments are necessary and strong side effects may be observed. In the dog, in contrast to ruminants and other species, luteolysis is not affected by hysterectomy. These observations led to the conclusion that rather paracrine/ autocrine mechanisms than endocrine mechanisms of PGF2a are responsible for luteal regression in the dog. The present experiments were designed to test for the capacity of canine CL to produce and respond to PGF2a. PGFS and PGFR from canine CL from days 5,15, 25, 35, 45, 65 after ovulation were isolated and cloned. A full length PGFS cDNA isolate contains an open reading frame (ORF) of 975 bases coding for a protein of 325 amino acids. The ORF of PGFR consists of 1101 nucleotides encoding a 367 amino acid protein. Both genes show a high homology (82-94%) compared to other species. Relatively weak PGFR mRNA expression was detected on day 5. It had increased by day 25 and remained constant thereafter. In situ hybridization analysis localized the PGFR to the cytoplasm of the luteal cells only, suggesting that those cells are the only targets of PGF2a in this species. Only negative results were obtained when testing for the expression of PGFS by RT-PCR. These data demonstrate that canine CL does not have the capacity to produce PGF2a by PGFS and suggest that luteal regression in non-pregnant bitches is not modulated by PGF2a. However, the PGFR seems to be constitutionally expressed, explaining the receptivity of canine Cl to exogenous PGF2a.
2005
2004
2002
2000
HENRY DRM&#39;IKIUGUMUTEMBEI. "HM Mutembei, ER Mutiga, VT Tsuma 2000: A Retrospective study on Reproductive Parameters of dogs. J South African Vet Ass 71(2): 115-117.". In: Animal Reproduction Science 109 (2008) 319. Elsevier; 2000.

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