Hellen N. Kariuki1, Titus I. Kanui, Abiy Yenesew, Nilesh B. Patel, Paul M. Mbugua. ANTINOCICEPTIVE ACTIVITIES OF TODDALIA ASIATICA ROOT EXTRACT USING THE FORMALIN TEST ON MICE

Citation:
N. DRKARIUKIHELLEN. "Hellen N. Kariuki1, Titus I. Kanui, Abiy Yenesew, Nilesh B. Patel, Paul M. Mbugua. ANTINOCICEPTIVE ACTIVITIES OF TODDALIA ASIATICA ROOT EXTRACT USING THE FORMALIN TEST ON MICE.". In: 14th World Congress on Pain -Milan 2012. International Association for the Study of Pain; 2012.

Abstract:

Aim of Investigation: Approximately 80% of the world population relies on traditional healers who use herbal remedies. Seventy percent of sub Saharan Africa is reported to be using herbal medicine for management of various medical conditions. Based on anecdotal evidence, herbal remedies used in most communities are claimed to be effective and there is need to evaluate their effectiveness for the benefits to the general population. Natural products still hold the promise for the future of drug discovery in the management pain. Analgesic substances have been purified from plants resulting in the identification of novel structures with known mechanism of actions. The roots and bark of Toddalia asiatica have been traditionally used in the treatment of pain. The aim of this study is to investigate the antinociceptive activities of T. asiatica root extract using the formalin test in mice.
Methods: Roots of Toddalia asiatica were sourced, air-dried, powdered and extraction done using dichloromethane and methanol in the ratio of 1:1. The extracts were then concentrated and reconstituted in 5% dimethylsulfoxide (DMSO) and 95% normal saline to achieve working concentrations of 50,100 and 200 mg / kg body weight. The experimental and control animals were injected intraperitoneally 1 hour prior to the experiment. 8 mice were used in each group and each animal was used once. Sensorimotor test was performed on each animal prior to the formalin test.
Results: None of the animals showed sensorimotor defect. The 50mg /kg dose showed no significant antinociceptive effect in either the early or the late phase of formalin test.The 100mg / kg dose showed highly significant antinociceptive effect (p < 0.001) in the late phase (15-30 mins) of formalin test while the 200mg / kg dose showed a significant antinociceptive effect (p < 0.01) in the early phase (0-5mins) of formalin test compared to the vehicle treated animals. The 200 mg / kg dose showed no significant effect in the late phase of formalin test.
Conclusions: These results suggest that the root extract of Toddalia asiatica has significant antinociceptive effects in the formalin test using mice.

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