Bio

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Publications


2011

Osanjo, G, Aluvaala E, Wadegu M, Bulimo W, Guantai AN, Mulaa F, Okalebo FA, Oluka M.  2011.  Carbohydrate active enzymes (Cazymes) as drug targets and tools for synthesis of medicinal compounds. Website
Osanjo, GO, Bulimo WD, Mulaa FJ.  2011.  Engineering the functional fitness of transglycosidases and glycosynthases by directed evolution. African Journal of Biotechnology . 10(10):1727-1735.
Mbogo, DM, Osanjo GO, Okoth MW, Mulaa FJ.  2011.  Recovery of carotenoids from microorganisms using edible vegetable oils. 1st International Scientific Conference, College Of Health Sciences, University Of Nairobi.
Ong’ayo, MN, Osanjo GO, Oluka MO.  2011.  Adherence to Anti-Tuberculosis Treatment among Paediatric Patients at Kenyatta National Hospital. 1st International Scientific Conference, College Of Health Sciences, University Of Nairobi.

2010

Karara, MW, Okalebo FA, Oluka MO, Ombega J, Guantai AN, Osanjo GO.  2010.  Comparative tolerability and efficacy of stavudine 30 mg versus stavudine 40 mg in patients on combination antiretroviral therapy in Kenya. Journal of AIDS and HIV Research. 2(2):024-031.

2009

Osanjo, GO, Muthike EW, Tsuma L, others.  2009.  A salt lake extremophile, Paracoccus bogoriensis sp. nov., efficiently produces xanthophyll carotenoids. African Journal of Microbiology Research . 3(8):426-433.

2008

G.O, O, N.M N.  2008.  Policies on Opioid Analgesics Hinder Management of Severe Pain Conditions in Kenya. The Pharmaceutical Journal of Kenya . 18(3):113-115.

2007

Osanjo, G, Dion M, Drone J, others.  2007.  Directed Evolution of the R-L-Fucosidase from Thermotoga maritima into an R-L-Transfucosidase. Biochemistry . 46:1022-1033.

2002

Oyamo, GO.  2002.  Identification by saturation mutagenesis of a single residue involved in the a-galactosidase agab regioselectivity. Abstract

a-Galactosidase AgaB from Bacillus stearothermophilus displays a major a(1~6) and a minor a(1~3) regioselectivity in hydrolysis and transglycosylation. Its corresponding gene, agaB, was subjected to saturation mutagenesis at codon 442 in order to change its regioselectivity. The mutant genes were cloned and expressed in Escherichia coli using pBTac2 as vector. The regioselective activity of the mutants was determined using thin layer chromatography and nuclear magnetic resonance spectroscopy. A single point mutation, G442R, resulted in a mutant displaying an a(1~2) regioselectivity. Other amino acid substitutions at this site also gave mutants with altered regioselectivity and transglycosylation profiles. This is the first demonstration that single point mutations can lead to a strong modification of the regioselectivity of a glycosyl hydrolase. The kinetic parameters of the enzyme variants were determined and a preliminary investigation of possible substrates for condensation reactions conducted.

2001

Dion, M, Osanjo G, Andre C, others.  2001.  Identification by saturation mutagenesis of a single residue involved in the a-galactosidase AgaB regioselectivity. Glycoconjugate Journal. 18:457–464.

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