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KIAMBI PROFKANGETHEE, A MRKABURIAHF, MWIHURIH PROFNJERUHF. "SANDWICH ENZYME IMMUNOASSAY FOR SPECIATION OF COOKED MEATS AND FOR DETECTING TRACE AMOUNTS OF ADULTERANTS IN PHYLOGENICALLY RELATED SPECIES.". In: journal. Kisipan, M.L.; 1994. Abstract
A double antibody enzyme linked immunosorbent assay for identification of thermostable muscle antigens of autoclaved meat samples is described. The assay differentiates heterologous thermostable muscle antigens from homologous at P 0.001. In model meat mixtures, the assay detects adulterants at the level of 1% at p0.001 even in phylogenetically related species such as buffalo and cattle.
KIAMBI PROFKANGETHEE. "SERODIAGNOSIS OF BOVINE CYSTICERCOSIS BY DETECTING LIVE Taenia saginata CYSTS USING A MONOCLONAL ANTIBODY- BASED ANTIGEN ELISA.". In: journal. Kisipan, M.L.; 2002. Abstract
{ An ante-mortem antigen ELISA based diagnosis of Taenia saginata cysticercosis was studied in artificially (n=24) and naturally (n=25) infected cattle with the objective of further validating the assay as a field diagnostic test. Based on total dissection as the definitive method of validity, the assay minimally detected 14 live cysticerci in artificially infected calves and 2 in natural infected steers. In natural infections, the minimum number of live cysticerci consistently detected by Ag-ELISA was 5 while in artificially infected calves it was above14. However, other animals with 12 and 17 live cysticerci in artificially infected calves and 1 and 2 in naturally infected steers escaped detection for unknown reasons. Animals harboring dead cysticerci gave negative results in the assay as were the case in non infected experimental control calves. There was a statistically significant positive linear correlation between Ag-ELISA optical density values and burdens of live cysticerci as obtained by total dissection of both artificially and natural infected calves (r=o.798
Kimani VN, Mitoko G, McDermott B, Grace D, Ambia J, Kiragu MW, Njehu AN, Sinja J. "Social and gender determinants of risk of cryptosporidiosis, an emerging zoonosis, in Dagoretti, Nairobi, Kenya.". 2012. AbstractWebsite

The aim of the study was to investigate the social and gender determinants of the risk of exposure to Cryptosporidium fromurban dairying in Dagoretti, Nairobi. Focus group discussions were held in six locations to obtain qualitative information on risk of exposure. A repeated cross-sectional descriptive study included participatory assessment and household questionnaires (300 randomly selected urban dairy farming households and 100 non-dairying neighbours). One hundred dairy households randomly selected from the 300 dairy households participated in an additional economic survey along with 40 neighbouring non-dairy households. We found that exposure to Cryptosporidium was influenced by gender, age and role in the household. Farm workers and people aged 50 to 65 years had most contact with cattle, and women had greater contact with raw milk. However, children had relatively higher consumption of raw milk than other age groups. Adult women had more daily contact with cattle faeces than adult men, and older women had more contact than older men. Employees had greater contact with cattle than other groups and cattle faeces, and most (77 %) were male. Women took more care of sick people and were more at risk from exposure by this route. Poverty did not affect the level of exposure to cattle but did decrease consumption of milk. There was no significant difference between men and women as regards levels of knowledge on symptoms of cryptosporidiosis infections or other zoonotic diseases associated with dairy farming. Awareness of cryptosporidiosis and its transmission increased significantly with rising levels of education. Members of nondairy households and children under the age of 12 years had significantly higher odds of reporting diarrhoea: gender, season and contact with cattle or cattle dung were not significantly linked with diarrhoea. In conclusion, social and gender factors are important determinants of exposure to zoonotic disease in Nairobi.

KIAMBI PROFKANGETHEE. "SOCIO-ECONOMIC CONSTRAINTS TO AGRICULTURAL PRODUCTIVITY IN KIAMBU DISTRICT.". In: journal. Kisipan, M.L.; 1998. Abstract
Sustainability in agriculture requires a better understanding of interactions within the production syatem. Diverse factors such as social structure, knowledge and information flow all as well as other bio[hyscical afctors interact with each other to determine agricultural productivity and sustainability. Researchers and extension agents find that they need to deal with issues that may be outside their area of specialization. An interdisciplinary approach provides a framework through which such support can be provided.
KIAMBI PROFKANGETHEE, MWANGI PROFGATHUMAJ, MWANGI PROFGATHUMAJ. "SPECIES IDENTIFICATION OF AUTOCLAVED MEAT SPECIES USING ANTISERA TO THERMOSTABLE MUSCLE ANTIGENS IN AN ENZYME IMMUNOASSAY.". In: journal. Kisipan, M.L.; 1987. Abstract
An enzyme immunoassay (EIA) suitable for use in identification of cooked and autoclaved meat samples using antisera to thermostable muscle antigens (TMA) is described. Goat antisera to TMA of various species were tested against homologous and heterologous partially purified thermostable muscle antigens (PTMA) in an indirect EIA. Goat anti-eland and anti-cattle TMA sera were the poorest in differentiating other species PTMAs. Identification of various species PTMAs could be achieved using a battery of goat anti-TMA sera, where homologous goat anti-TMA serum fails to differentiate some of the PTMAs tested.
KIAMBI PROFKANGETHEE, A MRKABURIAHF, ARIMI PROFMUTWIRIS. "STUDY OF THE MICROBIOLOGICAL QUALITY OF PROCESSED KENYAN HONEY: A PRELIMINARY REPORT.". In: journal. Kisipan, M.L.; 1990. Abstract
A preliminary study of microbiological quality of honey was carried out using 26 samples obtained from the National Bee Keeping Research Station. Total viable counts (TVC) of aerobic bacteria, yeasts and moulds, and Clostridium species were done. Of the 26 samples, 24 (92.3%) had a TVC ranging from 3 x10 -87 x 10 colony forming units (cfu) per gram of honey. Two samples did not yield any microorganisms. Of the 24 positive samples, 9 (37.5%) were found to contain Clostridium species per gram while eight (33.3%) were positive for moulds with counts ranging from 10-100 c.f.u / g. No yeasts were detected. In addition, three samples yielded the three types of microorganisms.

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