Publications


Submitted

Omuombo, C, Odada EO, Olago DO.  Submitted.  Coastal Erosion: A Natural Outlook-Geonvironmental Resources and Hazards. Abstract

This chapter focuses on the existing information on the hydrodynamics and sediment transport processes along the Kenyan coastline. Although the low-lying coastline is under threat from coastal erosion that has led to its destabilization, the factors are linked to local and global processes. Changes in land used for agriculture have led to increase sediment fluxes which have resulted in increase in turbidity and siltation. Other activities such as coral and mangrove harvesting, seawall construction, urbanization and lack of regulations on the construction of structures along of the coastline can be linked to the coastal erosion processes. Among the global factors, the Kenyan coastline has been affected by the extreme events such as the El Niño event of 1997/1998, which led to devastating effects such as an increase in sediment fluxes and turbidity, coral bleaching and mortality and substantial sea level rise. A 1.3 °C sea surface temperature rise on the western side of the Indian Ocean has been recorded since 1880; this makes the coastline vulnerable to the impacts of the predicted 6 °C temperature rise in East Africa due to climate change. It is estimated that the biggest coastal city of Mombasa will be 17% submerged by 2100 and the Tana delta will experience a 5% loss as a consequence of climate change due to the frequent storms that are anticipated. Although the 2004 global tsunami events did not have devastating effects on the Kenyan coast, the event hit the coastline at low tide and this led to the limited damage. In the management of the shoreline, currently an Integrated Coastal Zone Management strategy does not exist although efforts are underway to develop a shoreline management strategy that incorporates the principles of the integration in the management of the coastline. These efforts are encouraged by the success of the marine protected areas of Malindi and Watamu and the current co-management strategy adapted by the Ministry of Fisheries through the Beach Management Units that engages the resource users as equal partners in the management of the coastal resources.
Keywords

Urbanization;
Sediment flux;
El Niño;
Sea surface temperature;
Tsunami;
Mombasa;
Tana delta;
Marine protected areas;
Beach Management Units

2013

Paron, P, Olago DO, Omuto CT.  2013.  Kenya: A Natural Outlook: Methods and Applications. AbstractKenya: A Natural Outlook: Methods and Applications

Kenya is a thriving country in East Africa: its economy is largely based on the natural environment that frames the tourism sector, mainly through safaris and holidays on the coast. The natural environment also underpins the second largest industry: agriculture. Kenya’s social, technological, and industrial developments are a reference for many neighboring countries. Kenya plays a leading role in Africa and attracts huge amounts of investments. Furthermore, the humanitarian community has made Nairobi its base for international headquarters and regional offices. This makes Kenya a possible model for development and investment in its widest sense. This book aims at updating the holistic view on Kenya’s natural environment and resources. It provides a sound scientific introduction to this country’s physical and socioeconomic setting and its evolution through time and will appeal to a broad audience of students – in Kenya and abroad – as well as those working in the development and humanitarian sectors and to international donors looking for a scientific compendium on Kenya’s environment. Its structure and references allow the reader to deepen his or her knowledge of every theme touched on in the book.

2012

Olago, DO, Opiyo Akech N, Moses M.  2012.  Mineral, Oil and Gas Resources. Developments in Earth Surface process . Abstract

The mineral, oil and gas sectors have not played an important role in the economy of Kenya in the past, but the recent discovery of mineral sands and rare earth elements at the coast and oil in the Lokichar Basin in the northern part of the country are proving to be game changers in the mining, oil and gas sectors. The most important minerals mined in the past have been mainly industrial minerals with soda ash and fluorspar being the most important products. Significant tonnage of gold was mined in western parts of Kenya, but currently only minor exploration and production from the old mine sites is taking place. However, with the increased interest and the government resolve to improve mineral exploration, new mineral finds are possible. Exploration for oil and gas has been taking place in Kenya since the 1950s, but it is only recently that significant oil finds have been reported. The findings have inspired several companies to explore for oil and gas within all the major sedimentary basins in Kenya, namely, the Lokichar Basin, Turkana Basin, the Kerio and Baringo Basin, the Anza Basin, and the Lamu Basin.

2010

Junginger, A, Olago DO, Trauth MH.  2010.  The palaeo-lake Suguta and its importance for understanding lake level fluctuations in the East African Rift System. AbstractThe palaeo-lake Suguta and its importance for understanding lake level fluctuations in the East African Rift System

We studied the most recent dry-wet-dry cycle in the presently arid Suguta Valley in the Northern Kenya Rift where a 300-m-deep lake has formed during the so-called African Humid Period (AHP, 14.8-5.5 ka BP). Hydromodeling suggests that a relatively moderate 25% increase in precipitation was responsible for this dramatic lake level rise, which demonstrates the character of the Suguta Valley as an amplifier lake system. To detect the response of this lake system to climate fluctuations and their possible driving mechanisms with a focus on abrupt vs. gradual changes, we reconstructed a palaeo-lake level record for the time between 14 and 5 ka BP from up to 40 m thick lake-sediment sequences at three locations in the ~2,500 km2 palaeo-lake Suguta area. The sediments have been investigated for sediment characteristics such as grain size distributions, detrital and authigenic mineral phases, geochemical properties and microfossil assemblages. The stratigraphy for the sequences is based on 38 AMS 14C ages of biogenic carbonate and charcoal samples. Parallel dating of charcoal and snail-shell samples show age differences between 1,570-2,240 years suggesting a remarkably high, but well-defined reservoir age for palaeo-Lake Suguta most likely due to aged groundwater or 14C depleted CO2 degassing from active volcanoes. The observed reservoir effect highlights the potential problems while correlating East African lake level records with chronologies based on 14C datings of aquatic materials. The new chronology of water level fluctuations in the amplifier-lake Suguta indicates a general dry-wet-dry cycle synchronous with other lake chronologies during the AHP and multiple short-term fluctuations with abrupt lake level drops between 100 to 300 m within 100 to 200 years at 12.8-11.6 (during Younger Dryas time), 11.1-10.9; 10.4-10.2; 9.5-9.1; 9.0-8.8; 8.5-8.1 (during the 8.2 ka event) cal ka BP that seem to be linked with changes in the coupling between atmosphere and ocean systems. In contrast, the termination of the overall lake episode during the AHP shows a relatively gradual (linear) response to the reduction of solar heating due to insolation changes. The results of the analysis provides new insights into the sensitivity of Rift Valley lakes to climate change on different time scales. Abrupt climate shifts most likely caused dramatic environmental pressure on the biosphere, including humans that were already able to adopt relatively quickly to environmnetal change by new technologies during this time.

OCHIENG, DROLAGODANIEL.  2010.  Ochola, S.O., Eitel, B. and Olago, D.O. Vulnerability of schools to floods in Nyando River catchment, Kenya. The Journal of Disaster Studies, Policy and Management. doi:10.1111/j.0361-3666.2010.01167.x. Journal of Climatic Change. : The Journal of Disaster Studies, Policy and Management. doi:10.1111/j.0361-3666.2010.01167.x Abstract
Separation of midgut membrane proteins from the tick, Ambylomma variegatum, using a nonionic detergent (Triton X-114), resulted in two protein fractions, namely DET (detergent) and AQ (aqueous). In immunoblotting analysis with polyclonal antibodies against these fractions, 4 proteins (Mr approximately 27,000, 67,000, 86,000 and 95,000,) and 2 proteins (M, approximately 54,000 and 67,000) were detected in the DET and AQ fractions, respectively. Three of the DET fraction proteins Mr approximately 27,000, 67,000 and 95,000 were glycosylated since they bound to the lectin, concanavalin A. In 2-dimensional gel electrophoresis, the AQ and DET fraction proteins were found to be acidic in nature. In a series of bioassay experiments, rabbits were first immunised with both DET and AQ fractions and then infested with ticks. The egg batch weights of these ticks were reduced by 50% compared to control ticks. Furthermore, there was a significant reduction in the hatchability of eggs laid by ticks fed on rabbits previously immunised with both DET (14%) and AQ (33%) fractions. Based on the egg hatchability, the reproductive capacity of ticks was reduced by 77 and 48% by DET and AQ fractions, respectively.
OCHIENG, DROLAGODANIEL.  2010.  Vulnerability to epidemic malaria in the highlands of Lake Victoria basin: the role of climate change/variability, hydrology, health and socio-economic factors. Journal of Climatic Change.. Journal of Climatic Change. : Kenyan Veterinarian Abstract
Separation of midgut membrane proteins from the tick, Ambylomma variegatum, using a nonionic detergent (Triton X-114), resulted in two protein fractions, namely DET (detergent) and AQ (aqueous). In immunoblotting analysis with polyclonal antibodies against these fractions, 4 proteins (Mr approximately 27,000, 67,000, 86,000 and 95,000,) and 2 proteins (M, approximately 54,000 and 67,000) were detected in the DET and AQ fractions, respectively. Three of the DET fraction proteins Mr approximately 27,000, 67,000 and 95,000 were glycosylated since they bound to the lectin, concanavalin A. In 2-dimensional gel electrophoresis, the AQ and DET fraction proteins were found to be acidic in nature. In a series of bioassay experiments, rabbits were first immunised with both DET and AQ fractions and then infested with ticks. The egg batch weights of these ticks were reduced by 50% compared to control ticks. Furthermore, there was a significant reduction in the hatchability of eggs laid by ticks fed on rabbits previously immunised with both DET (14%) and AQ (33%) fractions. Based on the egg hatchability, the reproductive capacity of ticks was reduced by 77 and 48% by DET and AQ fractions, respectively.

2009

OCHIENG, DROLAGODANIEL.  2009.  Olago, D.O., Opere, A. and Barongo, J. Holocene palaeohydrology, groundwater and climate change in the lake basins of the Central Kenya Rift. Hydrological Sciences Journal, 54(4): 765-780.. Journal of Climatic Change. : Hydrological Sciences Journal, 54(4): 765-780. Abstract
Separation of midgut membrane proteins from the tick, Ambylomma variegatum, using a nonionic detergent (Triton X-114), resulted in two protein fractions, namely DET (detergent) and AQ (aqueous). In immunoblotting analysis with polyclonal antibodies against these fractions, 4 proteins (Mr approximately 27,000, 67,000, 86,000 and 95,000,) and 2 proteins (M, approximately 54,000 and 67,000) were detected in the DET and AQ fractions, respectively. Three of the DET fraction proteins Mr approximately 27,000, 67,000 and 95,000 were glycosylated since they bound to the lectin, concanavalin A. In 2-dimensional gel electrophoresis, the AQ and DET fraction proteins were found to be acidic in nature. In a series of bioassay experiments, rabbits were first immunised with both DET and AQ fractions and then infested with ticks. The egg batch weights of these ticks were reduced by 50% compared to control ticks. Furthermore, there was a significant reduction in the hatchability of eggs laid by ticks fed on rabbits previously immunised with both DET (14%) and AQ (33%) fractions. Based on the egg hatchability, the reproductive capacity of ticks was reduced by 77 and 48% by DET and AQ fractions, respectively.
OCHIENG, DROLAGODANIEL.  2009.  Garcin, Y., Juninger, A., Melnick, D., Olago, D.O., Strecker, M.R., Trauth, M.H. Late Pleistocene-Holocene rise and collapse of Lake Suguta, northern Kenya Rift. Quaternary Science Reviews, 28 (9-10): 911-925.. Journal of Climatic Change. : Quaternary Science Reviews, 28 (9-10): 911-925. Abstract
Separation of midgut membrane proteins from the tick, Ambylomma variegatum, using a nonionic detergent (Triton X-114), resulted in two protein fractions, namely DET (detergent) and AQ (aqueous). In immunoblotting analysis with polyclonal antibodies against these fractions, 4 proteins (Mr approximately 27,000, 67,000, 86,000 and 95,000,) and 2 proteins (M, approximately 54,000 and 67,000) were detected in the DET and AQ fractions, respectively. Three of the DET fraction proteins Mr approximately 27,000, 67,000 and 95,000 were glycosylated since they bound to the lectin, concanavalin A. In 2-dimensional gel electrophoresis, the AQ and DET fraction proteins were found to be acidic in nature. In a series of bioassay experiments, rabbits were first immunised with both DET and AQ fractions and then infested with ticks. The egg batch weights of these ticks were reduced by 50% compared to control ticks. Furthermore, there was a significant reduction in the hatchability of eggs laid by ticks fed on rabbits previously immunised with both DET (14%) and AQ (33%) fractions. Based on the egg hatchability, the reproductive capacity of ticks was reduced by 77 and 48% by DET and AQ fractions, respectively.
OCHIENG, DROLAGODANIEL.  2009.  Odada, E.O., Ochola, W.O. and Olago, D.O. Drivers of ecosystem change and their impacts on human well-being in Lake Victoria basin. African Journal of Ecology, 47(1): 46-54.. Journal of Climatic Change. : African Journal of Ecology, 47(1): 46-54. Abstract
Separation of midgut membrane proteins from the tick, Ambylomma variegatum, using a nonionic detergent (Triton X-114), resulted in two protein fractions, namely DET (detergent) and AQ (aqueous). In immunoblotting analysis with polyclonal antibodies against these fractions, 4 proteins (Mr approximately 27,000, 67,000, 86,000 and 95,000,) and 2 proteins (M, approximately 54,000 and 67,000) were detected in the DET and AQ fractions, respectively. Three of the DET fraction proteins Mr approximately 27,000, 67,000 and 95,000 were glycosylated since they bound to the lectin, concanavalin A. In 2-dimensional gel electrophoresis, the AQ and DET fraction proteins were found to be acidic in nature. In a series of bioassay experiments, rabbits were first immunised with both DET and AQ fractions and then infested with ticks. The egg batch weights of these ticks were reduced by 50% compared to control ticks. Furthermore, there was a significant reduction in the hatchability of eggs laid by ticks fed on rabbits previously immunised with both DET (14%) and AQ (33%) fractions. Based on the egg hatchability, the reproductive capacity of ticks was reduced by 77 and 48% by DET and AQ fractions, respectively.
OCHIENG, DROLAGODANIEL.  2009.  Bennett, M.R., Harris, J.W.K., Richmond, B.G., Braun, D.R., Mbua, E., Kiura, P., Olago, D., Kibunjia, M., Atieno, C., Behrensmeyer, A.K., Huddart, D. and Gonzalez, S. Early hominin foot morphology based on 1.5 million year-old footprints from Illeret, Ken. Journal of Climatic Change. : Science, 323: 1197-1201. DOI: 10.1126/science.1168132 Abstract
Separation of midgut membrane proteins from the tick, Ambylomma variegatum, using a nonionic detergent (Triton X-114), resulted in two protein fractions, namely DET (detergent) and AQ (aqueous). In immunoblotting analysis with polyclonal antibodies against these fractions, 4 proteins (Mr approximately 27,000, 67,000, 86,000 and 95,000,) and 2 proteins (M, approximately 54,000 and 67,000) were detected in the DET and AQ fractions, respectively. Three of the DET fraction proteins Mr approximately 27,000, 67,000 and 95,000 were glycosylated since they bound to the lectin, concanavalin A. In 2-dimensional gel electrophoresis, the AQ and DET fraction proteins were found to be acidic in nature. In a series of bioassay experiments, rabbits were first immunised with both DET and AQ fractions and then infested with ticks. The egg batch weights of these ticks were reduced by 50% compared to control ticks. Furthermore, there was a significant reduction in the hatchability of eggs laid by ticks fed on rabbits previously immunised with both DET (14%) and AQ (33%) fractions. Based on the egg hatchability, the reproductive capacity of ticks was reduced by 77 and 48% by DET and AQ fractions, respectively.
OCHIENG, DROLAGODANIEL.  2009.  Odada, E.O., Ochola, W.O. and Olago, D.O. Drivers of ecosystem change and their impacts on human well-being in Lake Victoria basin. African Journal of Ecology, 47(1): 46-54.. Journal of Climatic Change. : African Journal of Ecology, 47(1): 46-54. Abstract
Separation of midgut membrane proteins from the tick, Ambylomma variegatum, using a nonionic detergent (Triton X-114), resulted in two protein fractions, namely DET (detergent) and AQ (aqueous). In immunoblotting analysis with polyclonal antibodies against these fractions, 4 proteins (Mr approximately 27,000, 67,000, 86,000 and 95,000,) and 2 proteins (M, approximately 54,000 and 67,000) were detected in the DET and AQ fractions, respectively. Three of the DET fraction proteins Mr approximately 27,000, 67,000 and 95,000 were glycosylated since they bound to the lectin, concanavalin A. In 2-dimensional gel electrophoresis, the AQ and DET fraction proteins were found to be acidic in nature. In a series of bioassay experiments, rabbits were first immunised with both DET and AQ fractions and then infested with ticks. The egg batch weights of these ticks were reduced by 50% compared to control ticks. Furthermore, there was a significant reduction in the hatchability of eggs laid by ticks fed on rabbits previously immunised with both DET (14%) and AQ (33%) fractions. Based on the egg hatchability, the reproductive capacity of ticks was reduced by 77 and 48% by DET and AQ fractions, respectively.
OCHIENG, DROLAGODANIEL.  2009.  Odada, E.O., Ochola, W.O. and Olago, D.O. Understanding future ecosystem changes in Lake Victoria basin using participatory local scenarios. African Journal of Ecology, 47(1): 147-153.. Journal of Climatic Change. : African Journal of Ecology, 47(1): 147-153. Abstract
Separation of midgut membrane proteins from the tick, Ambylomma variegatum, using a nonionic detergent (Triton X-114), resulted in two protein fractions, namely DET (detergent) and AQ (aqueous). In immunoblotting analysis with polyclonal antibodies against these fractions, 4 proteins (Mr approximately 27,000, 67,000, 86,000 and 95,000,) and 2 proteins (M, approximately 54,000 and 67,000) were detected in the DET and AQ fractions, respectively. Three of the DET fraction proteins Mr approximately 27,000, 67,000 and 95,000 were glycosylated since they bound to the lectin, concanavalin A. In 2-dimensional gel electrophoresis, the AQ and DET fraction proteins were found to be acidic in nature. In a series of bioassay experiments, rabbits were first immunised with both DET and AQ fractions and then infested with ticks. The egg batch weights of these ticks were reduced by 50% compared to control ticks. Furthermore, there was a significant reduction in the hatchability of eggs laid by ticks fed on rabbits previously immunised with both DET (14%) and AQ (33%) fractions. Based on the egg hatchability, the reproductive capacity of ticks was reduced by 77 and 48% by DET and AQ fractions, respectively.
OCHIENG, DROLAGODANIEL.  2009.  Odada, E.O., Ochola, W.O. and Olago, D.O. Understanding future ecosystem changes in Lake Victoria basin using participatory local scenarios. African Journal of Ecology, 47(1): 147-153.. Journal of Climatic Change. : African Journal of Ecology, 47(1): 147-153. Abstract
Separation of midgut membrane proteins from the tick, Ambylomma variegatum, using a nonionic detergent (Triton X-114), resulted in two protein fractions, namely DET (detergent) and AQ (aqueous). In immunoblotting analysis with polyclonal antibodies against these fractions, 4 proteins (Mr approximately 27,000, 67,000, 86,000 and 95,000,) and 2 proteins (M, approximately 54,000 and 67,000) were detected in the DET and AQ fractions, respectively. Three of the DET fraction proteins Mr approximately 27,000, 67,000 and 95,000 were glycosylated since they bound to the lectin, concanavalin A. In 2-dimensional gel electrophoresis, the AQ and DET fraction proteins were found to be acidic in nature. In a series of bioassay experiments, rabbits were first immunised with both DET and AQ fractions and then infested with ticks. The egg batch weights of these ticks were reduced by 50% compared to control ticks. Furthermore, there was a significant reduction in the hatchability of eggs laid by ticks fed on rabbits previously immunised with both DET (14%) and AQ (33%) fractions. Based on the egg hatchability, the reproductive capacity of ticks was reduced by 77 and 48% by DET and AQ fractions, respectively.

2008

2007

Olago, DO, Umer, M; Ringrose S, Huntsman-Mapila P, Sow, E. H; Damnati B.  2007.  Palaeoclimate in Africa: An overview since the Last Glacial Maximum.
OCHIENG, DROLAGODANIEL.  2007.  Yanda, P., Wandiga, S., Kangalawe, R., Opondo, M., Olago, D., Githeko, A., Downs, T., Kabumbuli, R., Opere, A., Githui, F., Kathuri, J., Olaka, L., Apindi, E., Marshall, M., Ogallo, L., Mugambi, P., Kirumira, E., Nanyunja, R., Baguma, T., Sigalla, R. and . Aquatic Ecosystem Health and Management, 10(1):23-32.. : Earthscan, London. 978-1-84407-470-9 (*) Abstract
Separation of midgut membrane proteins from the tick, Ambylomma variegatum, using a nonionic detergent (Triton X-114), resulted in two protein fractions, namely DET (detergent) and AQ (aqueous). In immunoblotting analysis with polyclonal antibodies against these fractions, 4 proteins (Mr approximately 27,000, 67,000, 86,000 and 95,000,) and 2 proteins (M, approximately 54,000 and 67,000) were detected in the DET and AQ fractions, respectively. Three of the DET fraction proteins Mr approximately 27,000, 67,000 and 95,000 were glycosylated since they bound to the lectin, concanavalin A. In 2-dimensional gel electrophoresis, the AQ and DET fraction proteins were found to be acidic in nature. In a series of bioassay experiments, rabbits were first immunised with both DET and AQ fractions and then infested with ticks. The egg batch weights of these ticks were reduced by 50% compared to control ticks. Furthermore, there was a significant reduction in the hatchability of eggs laid by ticks fed on rabbits previously immunised with both DET (14%) and AQ (33%) fractions. Based on the egg hatchability, the reproductive capacity of ticks was reduced by 77 and 48% by DET and AQ fractions, respectively.
OCHIENG, DROLAGODANIEL.  2007.  Wandiga, S.O., Opondo, M., Olago, D.O., Githeko, A., Githui, F., Marshall, M., Downs, T., Opere, A., Yanda, P., Kangalawe, R., Kabumbuli, R., Kirumira, E., Kathuri, J., Apindi, A., Olaka, L., Ogallo, L., Mugambi, P., Sigalla, R., Nanyunja, R., Baguma, T. . Aquatic Ecosystem Health and Management, 10(1):23-32.. : Earthscan, London. 978-1-84407-469-3 (*) Abstract
Separation of midgut membrane proteins from the tick, Ambylomma variegatum, using a nonionic detergent (Triton X-114), resulted in two protein fractions, namely DET (detergent) and AQ (aqueous). In immunoblotting analysis with polyclonal antibodies against these fractions, 4 proteins (Mr approximately 27,000, 67,000, 86,000 and 95,000,) and 2 proteins (M, approximately 54,000 and 67,000) were detected in the DET and AQ fractions, respectively. Three of the DET fraction proteins Mr approximately 27,000, 67,000 and 95,000 were glycosylated since they bound to the lectin, concanavalin A. In 2-dimensional gel electrophoresis, the AQ and DET fraction proteins were found to be acidic in nature. In a series of bioassay experiments, rabbits were first immunised with both DET and AQ fractions and then infested with ticks. The egg batch weights of these ticks were reduced by 50% compared to control ticks. Furthermore, there was a significant reduction in the hatchability of eggs laid by ticks fed on rabbits previously immunised with both DET (14%) and AQ (33%) fractions. Based on the egg hatchability, the reproductive capacity of ticks was reduced by 77 and 48% by DET and AQ fractions, respectively.
OCHIENG, DROLAGODANIEL.  2007.  Veldkamp, A., Buis, E., Wijbrans, J.R., Olago, D.O., Boshoven, E.H., Mar. Aquatic Ecosystem Health and Management, 10(1):23-32.. : Quaternary Science Reviews 26: 2897-2912. Abstract
Separation of midgut membrane proteins from the tick, Ambylomma variegatum, using a nonionic detergent (Triton X-114), resulted in two protein fractions, namely DET (detergent) and AQ (aqueous). In immunoblotting analysis with polyclonal antibodies against these fractions, 4 proteins (Mr approximately 27,000, 67,000, 86,000 and 95,000,) and 2 proteins (M, approximately 54,000 and 67,000) were detected in the DET and AQ fractions, respectively. Three of the DET fraction proteins Mr approximately 27,000, 67,000 and 95,000 were glycosylated since they bound to the lectin, concanavalin A. In 2-dimensional gel electrophoresis, the AQ and DET fraction proteins were found to be acidic in nature. In a series of bioassay experiments, rabbits were first immunised with both DET and AQ fractions and then infested with ticks. The egg batch weights of these ticks were reduced by 50% compared to control ticks. Furthermore, there was a significant reduction in the hatchability of eggs laid by ticks fed on rabbits previously immunised with both DET (14%) and AQ (33%) fractions. Based on the egg hatchability, the reproductive capacity of ticks was reduced by 77 and 48% by DET and AQ fractions, respectively.
OCHIENG, DROLAGODANIEL.  2007.  Street-Perrott, F.A., Barker, P., Eglinton, G., Ficken, K., Wooller, M.J., Swain, D.L., Huang, Olago, D.O. and Huang, Y. (2007) Late Quaternary changes in carbon cycling on Mount Kenya, East Africa: a landscape-ecological perspective based on lake-sedimen. Aquatic Ecosystem Health and Management, 10(1):23-32.. : Quaternary Science Reviews, 26: 1838-1860. Abstract
Separation of midgut membrane proteins from the tick, Ambylomma variegatum, using a nonionic detergent (Triton X-114), resulted in two protein fractions, namely DET (detergent) and AQ (aqueous). In immunoblotting analysis with polyclonal antibodies against these fractions, 4 proteins (Mr approximately 27,000, 67,000, 86,000 and 95,000,) and 2 proteins (M, approximately 54,000 and 67,000) were detected in the DET and AQ fractions, respectively. Three of the DET fraction proteins Mr approximately 27,000, 67,000 and 95,000 were glycosylated since they bound to the lectin, concanavalin A. In 2-dimensional gel electrophoresis, the AQ and DET fraction proteins were found to be acidic in nature. In a series of bioassay experiments, rabbits were first immunised with both DET and AQ fractions and then infested with ticks. The egg batch weights of these ticks were reduced by 50% compared to control ticks. Furthermore, there was a significant reduction in the hatchability of eggs laid by ticks fed on rabbits previously immunised with both DET (14%) and AQ (33%) fractions. Based on the egg hatchability, the reproductive capacity of ticks was reduced by 77 and 48% by DET and AQ fractions, respectively.
OCHIENG, DROLAGODANIEL.  2007.  Street-Perrott, F.A., Barker, P., Eglinton, G., Ficken, K., Wooller, M.J., Swain, D.L., Huang, Olago, D.O. and Huang, Y. (2007) Late Quaternary changes in carbon cycling on Mount Kenya, East Africa: a landscape-ecological perspective based on lake-sedimen. Aquatic Ecosystem Health and Management, 10(1):23-32.. : Quaternary Science Reviews, 26: 1838-1860. Abstract
Separation of midgut membrane proteins from the tick, Ambylomma variegatum, using a nonionic detergent (Triton X-114), resulted in two protein fractions, namely DET (detergent) and AQ (aqueous). In immunoblotting analysis with polyclonal antibodies against these fractions, 4 proteins (Mr approximately 27,000, 67,000, 86,000 and 95,000,) and 2 proteins (M, approximately 54,000 and 67,000) were detected in the DET and AQ fractions, respectively. Three of the DET fraction proteins Mr approximately 27,000, 67,000 and 95,000 were glycosylated since they bound to the lectin, concanavalin A. In 2-dimensional gel electrophoresis, the AQ and DET fraction proteins were found to be acidic in nature. In a series of bioassay experiments, rabbits were first immunised with both DET and AQ fractions and then infested with ticks. The egg batch weights of these ticks were reduced by 50% compared to control ticks. Furthermore, there was a significant reduction in the hatchability of eggs laid by ticks fed on rabbits previously immunised with both DET (14%) and AQ (33%) fractions. Based on the egg hatchability, the reproductive capacity of ticks was reduced by 77 and 48% by DET and AQ fractions, respectively.
OCHIENG, DROLAGODANIEL.  2007.  Olago D.O., Umer, M., Ringrose, S., Huntsman-Mapila, P., Sow, E.H. and Damnati, B. (2007) Palaeoclimate of Africa: An overview since the last glacial maximum. Otter, L., Olago, D.O., and Niang, I. (Eds.) (2007). Global Change Processes and Impacts in Afri. Aquatic Ecosystem Health and Management, 10(1):23-32.. : East African Educational Publishers Ltd., Nairobi. (*) Abstract
Separation of midgut membrane proteins from the tick, Ambylomma variegatum, using a nonionic detergent (Triton X-114), resulted in two protein fractions, namely DET (detergent) and AQ (aqueous). In immunoblotting analysis with polyclonal antibodies against these fractions, 4 proteins (Mr approximately 27,000, 67,000, 86,000 and 95,000,) and 2 proteins (M, approximately 54,000 and 67,000) were detected in the DET and AQ fractions, respectively. Three of the DET fraction proteins Mr approximately 27,000, 67,000 and 95,000 were glycosylated since they bound to the lectin, concanavalin A. In 2-dimensional gel electrophoresis, the AQ and DET fraction proteins were found to be acidic in nature. In a series of bioassay experiments, rabbits were first immunised with both DET and AQ fractions and then infested with ticks. The egg batch weights of these ticks were reduced by 50% compared to control ticks. Furthermore, there was a significant reduction in the hatchability of eggs laid by ticks fed on rabbits previously immunised with both DET (14%) and AQ (33%) fractions. Based on the egg hatchability, the reproductive capacity of ticks was reduced by 77 and 48% by DET and AQ fractions, respectively.
OCHIENG, DROLAGODANIEL.  2007.  Olago, D.O., Marshall, M., Wandiga, S.O., Opondo, M., Yanda, P., Kangalawe, R., Githeko, A., Downs, T., Opere, A., Kabumbuli, R., Kirumira, E., Ogallo, L., Mugambi, P., Apindi, E., Githui, F., Kathuri, J., Olaka, L., Sigalla, R., Nanyunja, R., Baguma, T. . Aquatic Ecosystem Health and Management, 10(1):23-32.. : Ambio, 36(4): 350-358. Abstract
Separation of midgut membrane proteins from the tick, Ambylomma variegatum, using a nonionic detergent (Triton X-114), resulted in two protein fractions, namely DET (detergent) and AQ (aqueous). In immunoblotting analysis with polyclonal antibodies against these fractions, 4 proteins (Mr approximately 27,000, 67,000, 86,000 and 95,000,) and 2 proteins (M, approximately 54,000 and 67,000) were detected in the DET and AQ fractions, respectively. Three of the DET fraction proteins Mr approximately 27,000, 67,000 and 95,000 were glycosylated since they bound to the lectin, concanavalin A. In 2-dimensional gel electrophoresis, the AQ and DET fraction proteins were found to be acidic in nature. In a series of bioassay experiments, rabbits were first immunised with both DET and AQ fractions and then infested with ticks. The egg batch weights of these ticks were reduced by 50% compared to control ticks. Furthermore, there was a significant reduction in the hatchability of eggs laid by ticks fed on rabbits previously immunised with both DET (14%) and AQ (33%) fractions. Based on the egg hatchability, the reproductive capacity of ticks was reduced by 77 and 48% by DET and AQ fractions, respectively.
OCHIENG, DROLAGODANIEL.  2007.  Olago, D.O. and Odada, E.O. (2007) Sediment impacts in Africa. Aquatic Ecosystem Health and Management, 10(1):23-32.. : Hydrological Sciences Journal, 54(4): 765-780. Abstract
Separation of midgut membrane proteins from the tick, Ambylomma variegatum, using a nonionic detergent (Triton X-114), resulted in two protein fractions, namely DET (detergent) and AQ (aqueous). In immunoblotting analysis with polyclonal antibodies against these fractions, 4 proteins (Mr approximately 27,000, 67,000, 86,000 and 95,000,) and 2 proteins (M, approximately 54,000 and 67,000) were detected in the DET and AQ fractions, respectively. Three of the DET fraction proteins Mr approximately 27,000, 67,000 and 95,000 were glycosylated since they bound to the lectin, concanavalin A. In 2-dimensional gel electrophoresis, the AQ and DET fraction proteins were found to be acidic in nature. In a series of bioassay experiments, rabbits were first immunised with both DET and AQ fractions and then infested with ticks. The egg batch weights of these ticks were reduced by 50% compared to control ticks. Furthermore, there was a significant reduction in the hatchability of eggs laid by ticks fed on rabbits previously immunised with both DET (14%) and AQ (33%) fractions. Based on the egg hatchability, the reproductive capacity of ticks was reduced by 77 and 48% by DET and AQ fractions, respectively.

2006

OCHIENG, DROLAGODANIEL.  2006.  Geology, University of Nairobi. CV, June 2006. 7for African Diatomite Industry Limited (ADIL), November 2001 (with Mr. P. Okoth and Prof. N. Opiyo-Akech).. African Diatomite Industry Limited (ADIL), November 2001 (with Mr. P. Okoth and Prof. N. Opiyo-Akech).. : Earthscan, London. 978-1-84407-469-3 (*) Abstract
Separation of midgut membrane proteins from the tick, Ambylomma variegatum, using a nonionic detergent (Triton X-114), resulted in two protein fractions, namely DET (detergent) and AQ (aqueous). In immunoblotting analysis with polyclonal antibodies against these fractions, 4 proteins (Mr approximately 27,000, 67,000, 86,000 and 95,000,) and 2 proteins (M, approximately 54,000 and 67,000) were detected in the DET and AQ fractions, respectively. Three of the DET fraction proteins Mr approximately 27,000, 67,000 and 95,000 were glycosylated since they bound to the lectin, concanavalin A. In 2-dimensional gel electrophoresis, the AQ and DET fraction proteins were found to be acidic in nature. In a series of bioassay experiments, rabbits were first immunised with both DET and AQ fractions and then infested with ticks. The egg batch weights of these ticks were reduced by 50% compared to control ticks. Furthermore, there was a significant reduction in the hatchability of eggs laid by ticks fed on rabbits previously immunised with both DET (14%) and AQ (33%) fractions. Based on the egg hatchability, the reproductive capacity of ticks was reduced by 77 and 48% by DET and AQ fractions, respectively.

2005

OCHIENG, DROLAGODANIEL, ONYANGO PROFODADAERIC.  2005.  Odada, E.O. and Olago, D.O. (2005). Holocene Climatic, Hydrological and Environmental Oscillations in the Tropics with Special Reference to Africa. In: Pak Sum Low (Ed.) Climate Change and Africa. Cambridge University Press, Cambridge. January 2005. Cambridge University Press, Cambridge. January 2005. : Earthscan, London. 978-1-84407-469-3 (*) Abstract
Separation of midgut membrane proteins from the tick, Ambylomma variegatum, using a nonionic detergent (Triton X-114), resulted in two protein fractions, namely DET (detergent) and AQ (aqueous). In immunoblotting analysis with polyclonal antibodies against these fractions, 4 proteins (Mr approximately 27,000, 67,000, 86,000 and 95,000,) and 2 proteins (M, approximately 54,000 and 67,000) were detected in the DET and AQ fractions, respectively. Three of the DET fraction proteins Mr approximately 27,000, 67,000 and 95,000 were glycosylated since they bound to the lectin, concanavalin A. In 2-dimensional gel electrophoresis, the AQ and DET fraction proteins were found to be acidic in nature. In a series of bioassay experiments, rabbits were first immunised with both DET and AQ fractions and then infested with ticks. The egg batch weights of these ticks were reduced by 50% compared to control ticks. Furthermore, there was a significant reduction in the hatchability of eggs laid by ticks fed on rabbits previously immunised with both DET (14%) and AQ (33%) fractions. Based on the egg hatchability, the reproductive capacity of ticks was reduced by 77 and 48% by DET and AQ fractions, respectively.

2004

West, K, Bugenyi F, Kulindwa K, Olago DO, Odada EO.  2004.  East African RiĞ alley Lakes. Website
OCHIENG, DROLAGODANIEL, ONYANGO PROFODADAERIC.  2004.  Odada, E.O., Olago, D.O., Kulindwa, K., Ntiba, M. and Wandiga, S. (2004). Mitigation of environmental problems in Lake Victoria, East Africa: Causal chain and policy option analyses. Ambio, 33 (1-2): 13-23. February 2004. Ambio, 33 (1-2): 13-23. February 2004. : Earthscan, London. 978-1-84407-469-3 (*) Abstract
Separation of midgut membrane proteins from the tick, Ambylomma variegatum, using a nonionic detergent (Triton X-114), resulted in two protein fractions, namely DET (detergent) and AQ (aqueous). In immunoblotting analysis with polyclonal antibodies against these fractions, 4 proteins (Mr approximately 27,000, 67,000, 86,000 and 95,000,) and 2 proteins (M, approximately 54,000 and 67,000) were detected in the DET and AQ fractions, respectively. Three of the DET fraction proteins Mr approximately 27,000, 67,000 and 95,000 were glycosylated since they bound to the lectin, concanavalin A. In 2-dimensional gel electrophoresis, the AQ and DET fraction proteins were found to be acidic in nature. In a series of bioassay experiments, rabbits were first immunised with both DET and AQ fractions and then infested with ticks. The egg batch weights of these ticks were reduced by 50% compared to control ticks. Furthermore, there was a significant reduction in the hatchability of eggs laid by ticks fed on rabbits previously immunised with both DET (14%) and AQ (33%) fractions. Based on the egg hatchability, the reproductive capacity of ticks was reduced by 77 and 48% by DET and AQ fractions, respectively.
OCHIENG, DROLAGODANIEL.  2004.  Olago, D.O. and Odada, E.O. (2004). Palaeo-research in Africa: relevance to sustainable environmental management and significance for the future. In: R. Battarbee, F. Gasse and C. Stickley (Eds.) Past Climate Variability through Europe and Africa. Kluwer . Kluwer Academic Publishers, Dordrecht, The Netherlands.September 2004. : Earthscan, London. 978-1-84407-469-3 (*) Abstract
Separation of midgut membrane proteins from the tick, Ambylomma variegatum, using a nonionic detergent (Triton X-114), resulted in two protein fractions, namely DET (detergent) and AQ (aqueous). In immunoblotting analysis with polyclonal antibodies against these fractions, 4 proteins (Mr approximately 27,000, 67,000, 86,000 and 95,000,) and 2 proteins (M, approximately 54,000 and 67,000) were detected in the DET and AQ fractions, respectively. Three of the DET fraction proteins Mr approximately 27,000, 67,000 and 95,000 were glycosylated since they bound to the lectin, concanavalin A. In 2-dimensional gel electrophoresis, the AQ and DET fraction proteins were found to be acidic in nature. In a series of bioassay experiments, rabbits were first immunised with both DET and AQ fractions and then infested with ticks. The egg batch weights of these ticks were reduced by 50% compared to control ticks. Furthermore, there was a significant reduction in the hatchability of eggs laid by ticks fed on rabbits previously immunised with both DET (14%) and AQ (33%) fractions. Based on the egg hatchability, the reproductive capacity of ticks was reduced by 77 and 48% by DET and AQ fractions, respectively.

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